Increases in open quotient are widely assumed to cause changes in the amplitude of the first harmonic relative to the second (H1*–H2*), which in turn correspond to increases in perceived vocal breathiness. Empirical support for these assumptions is rather limited, and reported relationships among these three descriptive levels have been variable. This study examined the empirical relationship among H1*–H2*, the glottal open quotient (OQ), and glottal area waveform skewness, measured synchronously from audio recordings and high-speed video images of the larynges of six phonetically knowledgeable, vocally healthy speakers who varied fundamental frequency and voice qualities quasi-orthogonally. Across speakers and voice qualities, OQ, the asymmetry coefficient, and fundamental frequency accounted for an average of 74% of the variance in H1*–H2*. However, analyses of individual speakers showed large differences in the strategies used to produce the same intended voice qualities. Thus, H1*–H2* can be predicted with good overall accuracy, but its relationship to phonatory characteristics appears to be speaker dependent.