“…Ground beef cooking juices (Hague et al, 1994) and absence of redness in the meat juice are not reliable indicators for doneness (Nusimovich et al, 1979;Lyon, Berry, Soderberg, & Clinch, 2000). While changes in the optical (Xia, Weaver, Gerrard, & Yao, 2008;Pakula & Stamminger, 2012;Wold, 2016), electric (Brunton, Lyng, Zhang, & Jacquier, 2006), and structural (Garcia-Segovia, Andres-Bello, & Martinez-Monzo, 2007) properties of muscle foods can indicate the endpoint cooking temperature, such strategies have limited application in consumer kitchens. Although several studies have proposed that the residual activity of enzymes such as triose phosphate isomerase (Sair, Booren, Berry, & Smith, 1999), acid phosphatase (Lyon, Davis, Windham, & Lyon, 2001), and lactate dehydrogenase (Orta-Ramirez et al, 1996;Collins, Keeton, & Smith, 1991;Stalder, Smith, Keeton, & Smith, 1991 To engineer strategies to minimize PMB, the etiology and factors contributing to the incidence of PMB in beef need to be clearly characterized.…”