Measuring Chromosome Pairing During Homologous Recombination in Yeast

Joseph, Fraulin; Lee, So Jung; Bryant, Eric Edward; Rothstein, Rodney

doi:10.1007/978-1-0716-0644-5_18

Cited by 1 publication

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For synthetic complete (SC) medium lacking the appropriate amino acids, a custom synthetic complete amino acid mixture with 2× L-leucine was used as described in (Sherman, Fink, & Hicks, 1987). For I-SceI DSB induction, cells were grown overnight at 23°C in 7 ml of SC lactate liquid medium (Synthetic complete amino acid mixture; 3% glycerol; 2% lactic acid; 0.05% glucose; 150 mg/L adenine; adjusted to pH 5.7 with sodium hydroxide) in sterile 15 ml culture tubes (Joseph, Lee, Bryant, & Rothstein, 2021). The following morning, the cultures were diluted in 7 ml of fresh SC glycerol lactate medium to an optical density at a 600 nm wavelength (OD 600 ) of approximately 0.3.…”

Section: Media and Galactose Inductionmentioning

confidence: 99%

See 1 more Smart Citation

Temporal coordination between chromosome mobility and homologous recombination

Joseph

Lee

Bryant

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Homologous recombination (HR), a principal cellular pathway for double-strand break (DSB) repair, is linked to changes in chromosome movement. Although increased chromosome mobility in response to a DSB has been observed in a variety of species, its precise role in HR remains controversial. Here, we find that end resection, the recruitment of recombination proteins, increased chromosome mobility, the pairing of homologs and gene conversion are temporally linked in response to a DSB. In mre11Δ mutant cells, which exhibit a delay in the initial processing of a DSB, chromosome mobility and all subsequent recombination events are also delayed. Overexpression of the Dna2 nuclease suppresses the mre11Δ delay in end resection and restores the original timing of chromosome mobility and all subsequent downstream HR events. Thus, changing the timing of chromosome mobility results in a corresponding change in essential downstream HR events, reinforcing its mechanistic role in the DNA repair process.

show abstract

Section: Media and Galactose Inductionmentioning

confidence: 99%

“…Homolog pairing was performed as previously described in Joseph, et al (2021). Briefly, cells were grown overnight in 7 ml of SC lactate liquid medium, re-diluted the next morning to an OD 600 of 0.3 and allowed to grow at 23°C until they reached an OD 600 of approximately 0.5.…”

Section: Homolog Pairingmentioning

confidence: 99%