Measuring estrogens in women, men, and children: Recent advances 2012–2017

Smy, Laura; Straseski, Joely A.

doi:10.1016/j.clinbiochem.2018.05.014

Cited by 24 publications

(22 citation statements)

References 86 publications

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“…52 Analytical sensitivity and specificity at low concentrations of estradiol are needed for accurate measurement of estradiol levels, especially for postmenopausal women and women taking aromatase inhibitors. 52,53 Over the years, methods for measuring estradiol levels from serum have evolved. Early assays involved estradiol extraction, column chromatography, and measurement by RIA and then later, by direct RIAs utilizing buffers that release albumin-and SHBG-bound estradiol.…”

Section: Discussionmentioning

confidence: 99%

“…52 While advantages of immunoassays include eliminating the need for estradiol extraction, reducing costs, and increasing sample throughput, cross-reactivity with immunoassays make them unsuitable for detection of estradiol in patient populations with low estradiol concentrations. 53 In addition, RIAs required labor-intensive extractions, handling of radioactive material, and artifact from nonspecific binding to radioactivity. 54 Analytical methods such as LC or GC coupled with tandem mass spectrometry (MS/MS) are now being used more frequently for clinical samples as they have overcome some of the sensitivity and specificity limitations with RIAs, 52,53 even MS assays have variability between different assays.…”

Section: Discussionmentioning

confidence: 99%

“…53 In addition, RIAs required labor-intensive extractions, handling of radioactive material, and artifact from nonspecific binding to radioactivity. 54 Analytical methods such as LC or GC coupled with tandem mass spectrometry (MS/MS) are now being used more frequently for clinical samples as they have overcome some of the sensitivity and specificity limitations with RIAs, 52,53 even MS assays have variability between different assays. Varying levels of estradiol are reported when using assays with different cross-reactivities and specificities, particularly when comparing samples measured by RIA and LC/ MS or GC/MS.…”

Section: Discussionmentioning

confidence: 99%

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Systemic estradiol levels with low-dose vaginal estrogens

et al. 2019

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Objectives: To critically evaluate published systemic estradiol levels during use of low-dose vaginal estrogens considering detection method and estrogen dose; describe challenges with accurately measuring estradiol; and determine the normal estradiol level range in postmenopausal women. Methods: PubMed was searched for studies reporting systemic estradiol levels with lower-dose vaginal estrogens (25 mg estradiol or 0.3 mg conjugated equine estrogens). Estradiol levels at baseline and during treatment, area under the curve, and maximum estradiol concentrations were summarized by dose within assay type. A proposed range of systemic estradiol in normal, untreated, postmenopausal women was estimated by conservatively pooling means and standard deviations from published studies. Results: Mean basal estradiol levels were 3.1 to 4.9 pg/mL using liquid or gas chromatography/mass spectroscopy (LC or GC/MS/MS) with a range of undetectable to 10.5 pg/mL using radioimmunoassay. Systemic estradiol levels with vaginal estrogens reflected their doses as measured with LC or GC/MS/MS in different studies: 7.1 to 9.1 pg/mL and 16.7 to 22.7 pg/mL with a 25-mg softgel capsule insert and a tablet insert, respectively; 4.6 to 7.4 pg/mL and 6.6 to 14.8 pg/mL with a 10-mg softgel capsule and a tablet insert, respectively; and 3.6 to 3.9 pg/mL with a 4-mg softgel capsule insert. A mean systemic estradiol concentration ranging from undetectable to 10.7 pg/mL is proposed as an estimate for basal estradiol levels in normal, untreated, postmenopausal women. Systemic estradiol absorption may be influenced by the placement of estradiol higher (as with an applicator) versus lower (as without an applicator) in the vagina, as estradiol transport to the uterus would be more likely further away than closer to the introitus. Conclusion: Serum estradiol concentrations were generally lower when measured with more specific and sensitive assays. Estradiol absorption was dose-dependent, and may be influenced by dose, formulation, and positioning in the vagina. Very low systemic estradiol absorption with low/ultralow-dose vaginal estrogens may potentially decrease any adverse events that may be associated with higher doses of vaginal estrogens used for treating moderate to severe VVA due to less estradiol exposure.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Systemic estradiol levels with low-dose vaginal estrogens

et al. 2019

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“…These compounds are responsible for the development of female secondary sex characters and the health benefits of women, including the prevention of heart disease and osteoporosis [186]. They are also used for hormone therapy and oral contraceptives [187][188][189]. Estrogens can offer the risks because they act as endocrine disruptors (EDCs) resulting in fish feminization [190] and influencing plant or human health.…”

Section: Hormonesmentioning

confidence: 99%

Recent Progress on Luminescent Metal-Organic Framework-Involved Hybrid Materials for Rapid Determination of Contaminants in Environment and Food

et al. 2020

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The high speed of contaminants growth needs the burgeoning of new analytical techniques to keep up with the continuous demand for monitoring and legislation on food safety and environmental pollution control. Metal-organic frameworks (MOFs) are a kind of advanced crystal porous materials with controllable apertures, which are self-assembled by organic ligands and inorganic metal nodes. They have the merits of large specific surface areas, high porosity and the diversity of structures and functions. Latterly, the utilization of metal-organic frameworks has attracted much attention in environmental protection and the food industry. MOFs have exhibited great value as sensing materials for many targets. Among many sensing methods, fluorometric sensing is one of the widely studied methods in the detection of harmful substances in food and environmental samples. Fluorometric detection based on MOFs and its functional materials is currently one of the most key research subjects in the food and environmental fields. It has gradually become a hot research direction to construct the highly sensitive rapid sensors to detect harmful substances in the food matrix based on metal-organic frameworks. In this paper, we introduced the synthesis and detection application characteristics (absorption, fluorescence, etc.) of metal-organic frameworks. We summarized their applications in the MOFs-based fluorometric detection of harmful substances in food and water over the past few years. The harmful substances mainly include heavy metals, organic pollutants and other small molecules, etc. On this basis, the future development and possible application of the MOFs have prospected in this review paper.

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“…A gestatio alatt zajló ösztrogénmetabolom-változás megismerése perspektívát nyithat a különböző súlyosságú praeeclampsia, a gestatiós diabetes és az 1. trimeszteri fenyegető vetélés korai diagnózisához [26].…”

Section: Az öSztrogénmetabolom Kapcsolata Terhességi Kórképekkelunclassified

Az ösztrogénmetabolizmus várandósság alatt

et al. 2019

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The extensive metabolism of estrogen hormones, where oxidized forms, structural isomers and conjugated products appear in many tissues locally as well as in systemic circulation, is believed to be associated with a number of diseases. Targeted estrogen metabolomic studies have been largely associated with postmenopausal, malignant advert immune conditions. Although the role of estriol in maintaining pregnancy and the biological activity of estrogen metabolites is known, a relatively small number of publications have addressed the formation and transformation of these compounds during pregnancy. The aim of this study is to present in detail the formation and progression of estrogen metabolites during pregnancy and to summarize the knowledge of their role in undesirable processes occurring during gestation. Orv Hetil. 2019; 160(26): 1007–1014.

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Measuring estrogens in women, men, and children: Recent advances 2012–2017

Cited by 24 publications

References 86 publications

Systemic estradiol levels with low-dose vaginal estrogens

Systemic estradiol levels with low-dose vaginal estrogens

Recent Progress on Luminescent Metal-Organic Framework-Involved Hybrid Materials for Rapid Determination of Contaminants in Environment and Food

Az ösztrogénmetabolizmus várandósság alatt

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