Measuring Lymphocyte Transformation

Cooper, Ed

doi:10.1016/s0140-6736(66)90039-0

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…(3H)-TdR alone may interfere with cycling cells due to the inhibitory effect of radioactive thymidine. For such theoretical reasons Milton et a1 (1965) and Cooper (1966) argued that only a short time incubation can give reliable information on cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Spontaneous (³H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas

Kvaløy

Godal

Marton

et al. 1981

Scandinavian Journal of Haematology

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Spontaneous (3H)‐TdR incorporation was studied in cell suspensions from 64 patients with monoclonal B‐cell neoplasias. Among various incubation periods a long‐term (20 h) assay with (3H)‐TdR had the greatest discriminatory power versus non‐neoplastic lymph node cell suspensions. The (3H)‐TdR uptake correlated positively with cell volume, nuclear volume, and cells in S + G2 + M phase of the cell cycle. A high degree of heterogeneity with regard to (3H)‐TdR incorporation was found within several histological groups of the Kiel classification, especially in low‐grade malignant lymphomas of centroblastic/centrocytic origin and in the lymphoplasmacytoid groups. In highly malignant lymphomas (3H)‐TdR uptake was statistically, significantly higher than in lymphomas of low‐grade malignancy. Patients with localized disease (stages I and II) and those with ‘B’‐symptoms showed increased incorporation of radioactive thymidine as compared to patients with disseminated disease (stage IV). Data suggest that there exists correlation between prognosis as determined by histopathology and spontaneous uptake of (3H)‐TdR.

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Section: Discussionmentioning

confidence: 99%

Spontaneous (³H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas

Kvaløy

Godal

Marton

et al. 1981

Scandinavian Journal of Haematology

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“…(4) As was stated above, the modifications to the original culture technique of Caron et af. (1965) used in Experiment 4 were introduced, at the suggestion of Cooper (1966), to minimize errors which may result from the degradation of thymidine caused by leucocyte suspensions.…”

Section: Discussionmentioning

confidence: 99%

“…In these experiments there was no attempt to remove polyinorphonuclear leucocytes before culture, and the tritiated thymidine was added to the cultures at the onset. Cooper (1966) has pointed out that suspensions of leucocytes, especially polymorphonixclear leucocytes, can degrade thyinidine npidly to thymine and dlhydrothymine and has suggested that errors might be minimized if polymorphonuclear leucocytes were removed and the cultures exposed to the radioactive thymidine for only a short period of time. These suggestions were incorporated in the following technique.…”

Section: Expeririieiitmentioning

confidence: 99%

Spontaneous Lymphocyte Transformation in vitro: Negative Evidence from a Study of Thymidine Uptake

Caron

1967

Br J Haematol

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SUMMARY Studies of the uptake of tritiated thymidine by human peripheral lymphocytes cultured in autologous plasma or, after washing, in foetal calf serum have failed to provide evidence for the occurrence of spontaneous blastoid transformation during 1–7 days in vitro. Repeated washing of peripheral lymphocytes caused an inconstant reduction in their ability to respond to stimulation with PHA. The accuracy and sensitivity of the technique of measuring the degree of in vitro transformation activity of lymphocyte cultures by their uptake of radioactively labelled thymidine is increased by partially removing polymorphonuclear leucocytes from the leucocyte suspensions and by restricting exposure of the cultures to the radioactive thymidine to the final 1 hour of culture.

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“…Autoradiographic techniques do require a pure lymphocyte suspension, as has been stressed by Cooper (1966), since polymorphs in culture disintegrate, releasing enzymes which degrade thymidine, so making less available for uptake.…”

mentioning

confidence: 99%

Technique for lymphocyte transformation

Pentycross¹

1968

J Clin Pathol

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SYNOPSIS Current techniques for lymphocyte transformation are evaluated and criticised. A simple technique, designed to meet these criticisms, is described in detail, with particular reference to lymphocyte separation and scoring methods.Techniques for inducing morphological transformation of lymphocytes to large basophilic blast forms by non-specific mitogens, e.g. phytohaemagglutinin or 'pokeweed', or by specific antigens such as tetanus toxoid, have been described by

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Measuring Lymphocyte Transformation

Cited by 7 publications

References 7 publications

Spontaneous (³H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas

Spontaneous (³H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas

Spontaneous Lymphocyte Transformation in vitro: Negative Evidence from a Study of Thymidine Uptake

Technique for lymphocyte transformation

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Measuring Lymphocyte Transformation

Cited by 7 publications

References 7 publications

Spontaneous (3H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas

Spontaneous (3H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas

Spontaneous Lymphocyte Transformation in vitro: Negative Evidence from a Study of Thymidine Uptake

Technique for lymphocyte transformation

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Product

Resources

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Spontaneous (³H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas

Spontaneous (³H)‐Thymidine Uptake in Histological Subgroups of Human B‐Cell Lymphomas