Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry

Dam, Tarun K.; Talaga, Melanie L.; Fan, Ni; Brewer, C. Fred

doi:10.1016/bs.mie.2015.08.013

Cited by 18 publications

(10 citation statements)

References 22 publications

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“…In fact, attempts to detect the thermal effect in the demixing of both LCST and upper critical solution temperature behavior polymer solutions were made in the early development of the DSC method and showed prospective results . In addition, several works have shown that the measured macroscopic calorimetric values can be well correlated with the sum of microscopic molecular thermodynamic parameters . In our recent work, the demixing enthalpy recovery behavior is observed in a PNIPAM aqueous solution and interpreted as the recovering of swelling after the polymer coil collapses in the phase separation.…”

Section: Introductionmentioning

confidence: 90%

Entropic effect implication for change in polymer coils swelling state in the demixing enthalpy recovery of aqueous poly(vinyl methyl ether) solutions

Song

Lin

et al. 2018

J Polym Sci B Polym Phys

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Time-dependent demixing enthalpy recovery behavior of aqueous poly(vinyl methyl ether) (PVME) solutions exhibits distinct recovery characteristics in three concentration regions. The absence of recovery behavior below a water concentration of 38.3 wt % indicates that the PVME coil is in a globular state. The typically sigmoidal recovery behavior of demixing enthalpy above 38.3 wt % is ascribed to the reswelling of the collapsed polymer coils induced by the entropic effect. The increase in difference between the upper and lower limits indicates the continued swelling of the PVME coils. Above 65 wt %, a dominant diluting effect can be observed, and a much longer phase separation time is needed to reach the expected lower limit. In contrast, the recovery of demixing enthalpy in a wide range of water concentration (from 38.3 to 90 wt %) exhibits the same feature. The infrared spectroscopy results are in agreement with the above macroscopic differential scanning calorimetry results.

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Section: Introductionmentioning

confidence: 90%

Entropic effect implication for change in polymer coils swelling state in the demixing enthalpy recovery of aqueous poly(vinyl methyl ether) solutions

Song

Lin

et al. 2018

J Polym Sci B Polym Phys

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“…K d , dissociation constant; N, stoichiometry. subsequent binding of GbpC molecules is not affected in reverse analysis (21)(22)(23). Furthermore, the noncooperative adherence and saturation of the dextran lattice binding sites is confirmed by the decreasing slope values in the Hill plots ( Fig.…”

Section: Figmentioning

confidence: 63%

Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence

et al. 2018

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The high-resolution structure of glucan binding protein C (GbpC) at 1.14 Å, a sucrose-dependent virulence factor of the dental caries pathogen , has been determined. GbpC shares not only structural similarities with the V regions of AgI/II and SspB but also functional adherence to salivary agglutinin (SAG) and its scavenger receptor cysteine-rich domains (SRCRs). This is not only a newly identified function for GbpC but also an additional fail-safe binding mechanism for Despite the structural similarities with antigen I/II (AgI/II) and SspB of, GbpC remains unique among these surface proteins in its propensity to adhere to dextran/glucans. The complex crystal structure of GbpC with dextrose (β-d-glucose; Protein Data Bank ligand BGC) highlights exclusive structural features that facilitate this interaction with dextran. Targeted deletion mutant studies on GbpC's divergent loop region in the vicinity of a highly conserved calcium binding site confirm its role in biofilm formation. Finally, we present a model for adherence to dextran. The structure of GbpC highlights how artfully microbes have engineered the lectin-like folds to broaden their functional adherence repertoire.

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“…Examples include screening for lectins binding to a glycan microarray (Rillahan & Paulson, 2011; Smith, Song, & Cummings, 2010) or glycans binding to a lectin microarray (Pilobello & Mahal, 2007); NMR-based titrations where full-length carbohydrates or fragments thereof are titrated into lectin solutions and detected by NMR (Bewley & Shahzad-ul-Hussan, 2013). Similarly, as discussed later, carbohydrate binding can be detected by surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) (Dam, Talaga, Fan, & Brewer, 2016; Giannetti, 2011). …”

Section: Selection Of the Glycan Targetmentioning

confidence: 99%

Chemical and Biophysical Approaches for Complete Characterization of Lectin–Carbohydrate Interactions

Lusvarghi

Ghirlando

Davison

et al. 2018

Chemical Glycobiology Part B. Monitoring Glycans and Their Interactions

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Lectins are carbohydrate-binding proteins unrelated to antibodies or enzymes. While carbohydrates are present on all cells and pathogens, lectins are also ubiquitous in nature and their interactions with glycans mediate countless biological and physical interactions. Due to the multivalency found in both lectins and their glycan-binding partners, complete characterization of these interactions can be complex and typically requires the use of multiple complimentary techniques. In this chapter, we provide a general strategy and protocols for chemical and biophysical approaches that can be used to characterize carbohydrate-mediated interactions in the context of individual oligosaccharides, as part of a glycoprotein, and ending with visualization of interactions with whole virions.

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Measuring Multivalent Binding Interactions by Isothermal Titration Calorimetry

Cited by 18 publications

References 22 publications

Entropic effect implication for change in polymer coils swelling state in the demixing enthalpy recovery of aqueous poly(vinyl methyl ether) solutions

Entropic effect implication for change in polymer coils swelling state in the demixing enthalpy recovery of aqueous poly(vinyl methyl ether) solutions

Glucan Binding Protein C of Streptococcus mutans Mediates both Sucrose-Independent and Sucrose-Dependent Adherence

Chemical and Biophysical Approaches for Complete Characterization of Lectin–Carbohydrate Interactions

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