Measuring Novel Protein-Protein Binding with Surface Plasmon Resonance in the Physical Chemistry Lab

McFarland, BJ

doi:10.1021/bk-2018-1279.ch002

ACS Symposium Series

2018

DOI: 10.1021/bk-2018-1279.ch002

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Measuring Novel Protein-Protein Binding with Surface Plasmon Resonance in the Physical Chemistry Lab

BJ McFarland

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2022

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Surface plasmon resonance sensing in the advanced physics laboratory

Abdelhamid¹,

Kerrigan²,

Koopman³

et al. 2022

American Journal of Physics

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We present a set of experiments and computations suitable for introducing upper-level undergraduate physics and engineering students to the interdisciplinary field of nanoplasmonics for periods ranging from a week-long advanced laboratory session to a summer research project. The end product is a tunable optofluidic device capable of detecting changes in a fluid medium as low as 0.002 refractive index units. The sensing element—a thin gold film on a glass prism coupled to a microfluidic cell—owes its sensitivity to the bound nature of the surface plasmon–polariton waves that are resonantly excited by evanescently coupled light at the gold–fluid interface. Pedagogically, surface plasmon resonance (SPR) sensing immerses students in the rich physics of nanoscale optics and evanescent waves in constructing and operating a precision apparatus and in developing theoretical, analytical, and numerical models to aid both in the physical understanding and engineering optimization of the SPR sensor.

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Surface plasmon resonance sensing in the advanced physics laboratory

Abdelhamid¹,

Kerrigan²,

Koopman³

et al. 2022

American Journal of Physics

View full text Add to dashboard Cite

show abstract

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Measuring Novel Protein-Protein Binding with Surface Plasmon Resonance in the Physical Chemistry Lab

Cited by 1 publication

References 49 publications

Surface plasmon resonance sensing in the advanced physics laboratory

Surface plasmon resonance sensing in the advanced physics laboratory

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