Background:The Theoretical Domains Framework is an integrative framework that investigates the behavioural determinants and it's not well diffused in Brazil. Considering that the evidence-based practice implementation depends basically on behaviours related to adherence and to adhere or not to a practice, there are several barriers and enablers that needs to be overcome. The behaviour and its change are complex and influenced by several factorscognitive, affective, social, and environmental. Understanding these factors and how they act as barriers and/or facilitators to quality and ethical practices its crucial to change behaviours considered barriers to an implementation intervention. Therefore, the present methodological study aims to culturally adapt the Theoretical Domains Framework to the Brazilian Portuguese. Method: The adaptation was developed following six stages: translation, expert committee analysis, backtranslation, analysis by the original authors, translation, and expert committee analysis. To analyse the expert agreement was used the first-order agreement coefficient (AC1) with a significance level of α= 0.05. To address the reliability of the expert agreement, the AC1 ≥ 0,8 or the percentual agreement of ≥ 80% was adopted. Results: The final agreement between experts was achieved (≤0,005) and the framework was adapted to the Brazilian Portuguese. Conclusions: With the availability of the framework to Brazilian researchers and professionals, we aim contribute to the investigation of factors that act as barriers and enablers on the evidence-based practice implementation in several areas, improving the quality of the healthcare in Brazil.