Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Watts, Christina A.; Haupt, Alexandra; Smith, Jordan; Welch, Emily; Malik, Aalia; Giacomino, Roman; Walter, Dinah; Mayunddza, Nhlalala; Shemery, Ashley; Caldwell, Heather K.; Novak, Colleen M.

doi:10.3791/64264

Cited by 4 publications

(7 citation statements)

References 39 publications

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“…Furthermore, the degree of inflammation seems to be linked directly to oxytocin in a dose-dependent manner in rats ( Juif and Poisbeau, 2013 ; Bhumbra et al, 2009 ; Erbas et al, 2014 ; Indrayan et al, 2023 ). Exogenous oxytocin may have the ability to lower muscle temperature in mice and rats ( Watts et al, 2022 ), inevitably highlighting the importance of temperature and other variables. Based on this data, it might be possible that oxytocin later could serve as a complementary therapy for sports injuries.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the time animal studies control for auditory input in relation to oxytocin. At the time of writing this review, there is not enough information about the changes due to said input considering this neuropeptide, but it seems that the relationship between oxytocin and auditory input is species-dependent, namely, SD rats, wolves (a strong hierarchical order), cats (somewhat owner-dependent response), and zebra finch (mesotocin-spatial location of birds among each other) behaved differently ( Watts et al, 2022 ; Baran et al, 2016 ; Fitzpatrick and Morrow, 2020 ; Hosgorler et al, 2020 ; Nagasawa et al, 2022 ; Wirobski et al, 2023 ). Humans commonly use singing in many rituals, and it is part of every culture throughout the world.…”

Section: Discussionmentioning

confidence: 99%

“…Frontiers in Physiology frontiersin.org muscle temperature in mice and rats (Watts et al, 2022), inevitably highlighting the importance of temperature and other variables.…”

Section: Pain and Analgesiamentioning

confidence: 99%

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Systematic review: pain, cognition, and cardioprotection—unpacking oxytocin’s contributions in a sport context

Szabó,

Bonet,

Hetényi

et al. 2024

Front. Physiol.

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IntroductionThis systematic review investigates the interplay between oxytocin and exercise; in terms of analgesic, anti-inflammatory, pro-regenerative, and cardioprotective effects. Furthermore, by analyzing measurement methods, we aim to improve measurement validity and reliability.MethodsUtilizing PRISMA, GRADE, and MECIR protocols, we examined five databases with a modified SPIDER search. Including studies on healthy participants, published within the last 20 years, based on keywords “oxytocin,” “exercise” and “measurement,” 690 studies were retrieved initially (455 unique records). After excluding studies of clinically identifiable diseases, and unpublished and reproduction-focused studies, 175 studies qualified for the narrative cross-thematic and structural analysis.ResultsThe analysis resulted in five categories showing the reciprocal impact of oxytocin and exercise: Exercise (50), Physiology (63), Environment (27), Social Context (65), and Stress (49). Exercise-induced oxytocin could promote tissue regeneration, with 32 studies showing its analgesic and anti-inflammatory effects, while 14 studies discussed memory and cognition. Furthermore, empathy-associated OXTR rs53576 polymorphism might influence team sports performance. Since dietary habits and substance abuse can impact oxytocin secretion too, combining self-report tests and repeated salivary measurements may help achieve precision.DiscussionOxytocin’s effect on fear extinction and social cognition might generate strategies for mental training, and technical, and tactical development in sports. Exercise-induced oxytocin can affect the amount of stress experienced by athletes, and their response to it. However, oxytocin levels could depend on the type of sport in means of contact level, exercise intensity, and duration. The influence of oxytocin on athletes’ performance and recovery could have been exploited due to its short half-life. Examining oxytocin’s complex interactions with exercise paves the way for future research and application in sports science, psychology, and medical disciplines.Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=512184, identifier CRD42024512184

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Systematic review: pain, cognition, and cardioprotection—unpacking oxytocin’s contributions in a sport context

Szabó,

Bonet,

Hetényi

et al. 2024

Front. Physiol.

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“…After at least 2 wk of recovery from surgery, rats were habituated to the experimental procedure (i.e. moved to the testing room, exposed to towel stimulus with no odor, and transponder temperatures measured using the reader) with no experimental stimuli [ 10 , 34 ]. For measurement of the predator odor-induced thermogenic response, rats were exposed to a small fragment (~2X2 inches) of a towel that had been housed with ferrets for 2–3 wk (Marshall Bioresources, North Rose, NY), or a “blank” odorless, control towel.…”

Section: Methodsmentioning

confidence: 99%

“…Though time of day, recent activity, ambient temperature [ 36 ], habituation to the environment, and potentially genetic background can all add variance to muscle physiology and thermoregulation [ 10 , 30 , 34 ], differences in baseline muscle temperature likely stemmed from differences in transponder placement between investigators. This was mitigated by calculation of baseline-corrected temperature area under the curve (AUC), computed using the trapezoidal method correcting for baseline temperature in each rat, as described previously [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

Reduced contextually induced muscle thermogenesis in rats with calorie restriction and lower aerobic fitness but not monogenic obesity

et al. 2023

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We have previously identified predator odor as a potent stimulus activating thermogenesis in skeletal muscle in rats. As this may prove relevant for energy balance and weight loss, the current study investigated whether skeletal muscle thermogenesis was altered with negative energy balance, obesity propensity seen in association with low intrinsic aerobic fitness, and monogenic obesity. First, weight loss subsequent to 3 wk of 50% calorie restriction suppressed the muscle thermogenic response to predator odor. Next, we compared rats bred based on artificial selection for intrinsic aerobic fitness – high- and low-capacity runners (HCR, LCR) – that display robust leanness and obesity propensity, respectively. Aerobically fit HCR showed enhanced predator odor-induced muscle thermogenesis relative to the less-fit LCR. This contrasted with the profound monogenic obesity displayed by rats homozygous for a loss of function mutation in Melanocortin 4 receptor ( Mc4r K3a,4X/K314X rats), which showed no discernable deficit in thermogenesis. Taken together, these data imply that body size or obesity per se are not associated with deficient muscle thermogenesis. Rather, the physiological phenotype associated with polygenic obesity propensity may encompass pleiotropic mechanisms in the thermogenic pathway. Adaptive thermogenesis associated with weight loss also likely alters muscle thermogenic mechanisms.

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Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Cited by 4 publications

References 39 publications

Systematic review: pain, cognition, and cardioprotection—unpacking oxytocin’s contributions in a sport context

Systematic review: pain, cognition, and cardioprotection—unpacking oxytocin’s contributions in a sport context

Reduced contextually induced muscle thermogenesis in rats with calorie restriction and lower aerobic fitness but not monogenic obesity

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