Measuring Soluble Ion Concentrations (Na+, K+, Cl−) in Salt-Treated Plants

Munns, Rana; Wallace, Patricia A.; Teakle, Natasha L.; Colmer, Timothy D.

doi:10.1007/978-1-60761-702-0_23

Cited by 171 publications

(90 citation statements)

References 12 publications

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“…Leaves were digested in 10 mL of 1% (w/w) nitric acid (70% [w/w] Nitric Acid; Chem-Supply NA001-500M, Gillman) at 70°C for 8 h. Samples were diluted in milliQ water at a 1:5 or 1:10 ratio, and Na + and K + content was determined against the appropriate Na + and K + standard (100:1,000-500:1,000 mM NaCl:KCl) using flame photometry (Model 420 Flame Photometer, Sherwood Scientific). Ion content was calculated per gram of dry mass as described by Munns et al (2010).…”

Section: Determination Of Sodium and Potassium Contentmentioning

confidence: 99%

Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice

et al. 2015

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Salinity affects a significant portion of arable land and is particularly detrimental for irrigated agriculture, which provides onethird of the global food supply. Rice (Oryza sativa), the most important food crop, is salt sensitive. The genetic resources for salt tolerance in rice germplasm exist but are underutilized due to the difficulty in capturing the dynamic nature of physiological responses to salt stress. The genetic basis of these physiological responses is predicted to be polygenic. In an effort to address this challenge, we generated temporal imaging data from 378 diverse rice genotypes across 14 d of 90 mM NaCl stress and developed a statistical model to assess the genetic architecture of dynamic salinity-induced growth responses in rice germplasm. A genomic region on chromosome 3 was strongly associated with the early growth response and was captured using visible range imaging. Fluorescence imaging identified four genomic regions linked to salinity-induced fluorescence responses. A region on chromosome 1 regulates both the fluorescence shift indicative of the longer term ionic stress and the early growth rate decline during salinity stress. We present, to our knowledge, a new approach to capture the dynamic plant responses to its environment and elucidate the genetic basis of these responses using a longitudinal genome-wide association model.

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Section: Determination Of Sodium and Potassium Contentmentioning

confidence: 99%

Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice

et al. 2015

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“…Around 100 mg of leaf, leaf sheath and root samples were taken and dried in oven and digested in 0.5 ml of 0.5N HNO 3 for 2 h at 80 °C as per the method of Munns et al 2010). The digested samples were centrifuged and 100 µl of the supernatant was diluted 100 times by water.…”

Section: Measurement Of Ionsmentioning

confidence: 99%

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Rana

Ram

Nehra

et al. 2016

Cereal Research Communications

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In the present investigation, expression of genes related to Na + exclusion such as salt overly sensitive (TaSOS1) and Na + /H + antiporter (TaNHX1) and proline accumulation such as pyrroline-5-carboxylate reductase (P5CR) and glutamate synthase (GOGAT) was studied in seedlings of Kharchia 65 (Kh 65, salt tolerant) and HD 2009 (sensitive) under salt stress (ECe, 12 dSm -1 ) and controlled conditions. As compared to HD 2009, Kh 65 showed significantly lower accumulation of Na + (p < 0.01) and higher accumulation of proline (p < 0.05) in leaf blade under salt stress. The relative expression of TaSOS1 increased significantly (p < 0.001) in roots of Kh 65 (4.31-fold) while it decreased in HD 2009. There was significantly higher (p < 0.01) relative expression of TaNHX1 (27.57-fold) in leaf and 3.07-fold in roots of Kh 65 as compared to 3.65-and 0.87-fold increase in leaf and roots of HD 2009, respectively, under salt stress. There was significantly (p < 0.05) higher accumulation of proline as compared to HD 2009 in leaf tissues. There was significantly higher (p < 0.01) expression of P5CR (5.23-fold in leaf and 8.77-fold in the root) and glutamate synthase (6.0-fold in roots) in Kh 65 as compared to HD 2009. The study demonstrated that upregulation of genes for Na + exclusion in root and compartmentation in leaf and increased proline concentration are associated with tolerance to salinity stress in wheat. The information will be useful for improving wheat genotypes for salt tolerance.

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“…The leaves were rinsed with distilled water and oven-dried at 70 C, and the dried tissue samples were ground. Na + and K + were extracted from the samples by shaking 30 mg of ground leaf material in 5 ml of 0.5 M HNO 3 for 2 d according to Munns et al (2010). Na + and K + were measured using a flame photometer (BWB, UK) and verified by analysis of standards.…”

Section: Hydroponic Experimentsmentioning

confidence: 99%

Genome Structure and Salt Stress Response of Some Segregated Lines from Wheat and Tritipyrum Crosses

2013

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SummaryWe evaluated the genome structure and salt stress response of BC 1 F 6 lines derived from a cross between an Iranian salt tolerant bread wheat cultivar Roushan (2n=6x=42, AABBDD) and a synthetic allohexaploid Tritipyrum (2n=6x=42, AABBE b E b ). Sixteen lines (L1 to L16) and their parents were studied for their chromosome composition, K + : Na + ratio, and relative dry mass (RDM) by growing them in nutrient solution containing 0 (control) or 100 mM NaCl (treatment). Among the studied lines, eight wheat Thinopyrum bessarabicum (E b genome) disomic substitution lines were identified using PCR-based Landmark Unique Gene (PLUG) markers and cytogenetic analysis. Ten (37%) of 27 PLUG markers used were informative for E b chromosomes 1, 2, 3, 4, and 6 in a wheat background. PLUG markers also revealed that one line, L9, was a disomic substitution line DS2E b (2B), and that seven lines, L3, L4, L7, L8, L14, L15, and L16, were DS6E b (6D). There were large differences in morphological traits and salinity tolerance even among DS6E b (6D) substitutions or untranslocated lines, due perhaps to unidentified rearrangements or small introgressions.Key words Disomic substitution line, EST-based marker, Thinopyrum bessarabicum, Wheat. Domestication and modern plant breeding have narrowed the genetic base of bread wheat (Triticum aestivum; 2n=6x=42, AABBDD), which could restrict future crop improvement (Reif et al. 2005). Plants are constantly exposed to environmental stresses that limit their growth and productivity. Seven percent of the land in Iran contain salt either in the irrigation water or in soil (Zohary 1973). Wheat (Triticum aestivum L.) is the most important and widely adapted cereal in this country, and breeding for salt tolerance is one of the most efficient ways to increase its yield.To increase the salt tolerance of wheat, most of the attention has been focused on identifying genetic sources with low rates of transport of Na + to leaves, high selectivity for K + over Na + (i.e. Na + exclusion ), and higher performance . Wide hybridization of some halophytic wild species in the Triticeae with wheat has been proposed as a strategy to increase salt tolerance beyond the existing cultivated wheat gene pool (Colmer et al. 2006). Amongst the wild relatives, tall wheatgrass species, e.g. Thinopyrum bessarabicum (Save ex Rayss) A Löve (2n=2x=14,and Lophopyrum elongatum (Host) A. Löve (2n=2x=14, EE), are important sources of genes for agronomically desirable traits, particularly salt tolerance (Dvorak and Ross 1986). Wheat Th. bessarabicum amphiploids (Tritipyrums) have been produced and one such amphiploid was shown to survive in 250 mM NaCl, equivalent to halfstrength sea water (Forster et al. 1988). The salt tolerance potential of Tritipyrum and some of the disomic addition and substitution lines derived from a cross between wheat and Tritipyrum have been evaluated, and the positive contribution of 5E b addition lines has been reported (King et al. 1997a).Cytogenetic methods and molecular markers have been used in s...

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Measuring Soluble Ion Concentrations (Na+, K+, Cl−) in Salt-Treated Plants

Cited by 171 publications

References 12 publications

Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice

Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Genome Structure and Salt Stress Response of Some Segregated Lines from Wheat and Tritipyrum Crosses

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Measuring Soluble Ion Concentrations (Na+, K+, Cl−) in Salt-Treated Plants

Cited by 171 publications

References 12 publications

Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice

Integrating Image-Based Phenomics and Association Analysis to Dissect the Genetic Architecture of Temporal Salinity Responses in Rice

Expression of genes related to Na+exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Genome Structure and Salt Stress Response of Some Segregated Lines from Wheat and Tritipyrum Crosses

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Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress