Measuring the antioxidant activity of olive oil mill wastewater using chemiluminescence

Atanassova, D.; Kefalas, Panagiotis; Psillakis, Elefteria

doi:10.1016/j.envint.2004.10.003

Cited by 42 publications

(24 citation statements)

References 27 publications

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“…These include Folin-Ciocalteu (F-C) method, Folin-Denis (F-D) method [2], colorimetry (using iron salts), ultraviolet absorbance and permanganate titration as shown in Figure 1. Mostly, the F-C method is more preferred to other quantification methods due to its accuracy [2,12,13,14]. [2,8].…”

Section: Discussionmentioning

confidence: 99%

An Overview of Natural Plant Antioxidants: Analysis and Evaluation

Mohammed¹,

Tijani²,

Abubakar³

et al. 2013

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Antioxidative compounds play a vital role to inhibit the oxidation of oxidizable products and influences the body defense system against Reactive Oxygen Species (ROS), which are harmful byproducts engendered during normal aerobic cellular respiration. ROS are specialized class of highly reactive molecules that originates during oxygen metabolism, whereas extensive evidences indicate the involvement of ROS in the development of degenerative diseases in humans. Despite the fact that synthetic antioxidants such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are threat to human's health; they are widely employed as chemical preservatives to avert potential deterioration of food products. Several toxicological findings reveal that natural antioxidants are capable of providing protection against free radical-related diseases, cancer and other cardiovascular infections. In cognizance with these facts, natural antioxidant attracts a great deal of research interest; therefore, this review provides a comprehensive and an up-to-date overview of the three most essential natural plant antioxidants; their antioxidative properties, methods of analysis and evaluation. These Polyphenols comprise: Phenolic acid, an essential polyphenol that are broadly dispersed in plants to exert positive influence on oxidative stress; Flavonoid, an ubiquitous antioxidant that functions in free radical scavenging, metal ion chelation and enzymes inhibition to suppress the synthesis of free radicals; and Tannic acid, a water soluble anti-nutrient that acts to precipitate alkaloids & proteins, reduce mineral & vitamin utilization and restrict the activities of digestive enzymes. In conclusion, a variety of plant species were characterized to indicate in vitro antioxidative properties.

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Section: Discussionmentioning

confidence: 99%

An Overview of Natural Plant Antioxidants: Analysis and Evaluation

Mohammed¹,

Tijani²,

Abubakar³

et al. 2013

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“…Therefore, fast, simple screening of the antioxidant capacity of OMW residues is required, not only to assess their environmental toxicity and the possibility of treating or disposing the waste produced, but also to determine the amount of natural antioxidants present because the olive oil residues can be a cheap source of natural antioxidants. Atanassova et al [18] have investigated a rapid, simple and sensitive procedure for determining the total phenolic/antioxidant levels of olive oil mill wastewater using Co(II)/ethylenediaminetetracetic acid-induced luminol chemiluminescence. They tested the simple phenolic compound content of olive oil wastewater samples as a function of the extraction system (two-and three-phase centrifugation system).…”

Section: Luminol As a Chemiluminescent Reagentmentioning

confidence: 99%

“…The literature contains numerous publications [17,18] that show that luminol chemiluminescence is widely used as a sensitive assay to monitor free radicals and reactive metabolites. Some of the reported sources of free radicals are hydrogen peroxide and organic peroxides and it has been demonstrated that the presence of many transition metal cations [Co(II), Cu(I), Cu(II), Fe(II) and Fe(III)] catalyze the decomposition of hydrogen peroxide into hydroxyl radicals:…”

Section: Luminol As a Chemiluminescent Reagentmentioning

confidence: 99%

“…Characterization of thermooxidative stability [24] Luminol and lucigenin Antioxidant effect of hydroxytyrosol [25] Luminol Determination of total antioxidant levels of olive mill wastewater [18] Luminol Evaluation how ultrasonic irradiation can be used to reduce antioxidant activity of olive oil mill wastewater [26] Luminol Effects of an olive oil-based lipid emulsion [27] TCPO Determination of peroxide value [28] Rat liver microsomes Effect of polyphenolic compounds on the non-enzymatic lipid peroxidation [29] Potassium superoxide in dimethylsulfoxide Potential adulteration [30] 18-crown-6/dimethylsulfoxide/KO 2 Antioxidant activity of genistein and oleuropein [31] These studies add the biochemical properties of olive oil components by showing that the phenolic components selectively inhibit the 5-LO (leukotriene) pathway of arachidonate metabolism in activated leukocytes but do not affect the cyclo-oxygenase pathway. As far as free radical scavenging is concerned, de la Puerta et al have shown that oleuropein, hydroxytyrosol, tyrosol and caffeic acid can quench the superoxide chemiluminescence in phorbol myristate acetate-stimulated cells and scavenge hydrogen peroxide.…”

Section: Lucigenin As a Chemiluminescent Reagentmentioning

confidence: 99%

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Chemiluminescent Methods in Olive Oil Analysis

Navas

Jiménez

2007

J Americ Oil Chem Soc

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The present review describes chemiluminescence applications to olive oil analysis and olive mill wastewater and covers the literature published in recent years. The article has been divided into several sections that discuss specific applications for determining phenolic compounds, antioxidant activity, radical scavenging, oxidative stability and the detection of possible adulteration by seed oils. The advantages and disadvantages of the chemiluminescence detection systems for these purposes are evaluated.

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“…For the phenol measurements 15 ml of wastewater samples were taken at 0, 0.5, 1,2, 4,8, and 24h intervals, while COD was measured at 0,4, 12 and 24 h. The sorption capacity (q) was defined as: q= (C sorbate in the wastewater (mg/L) at zero and interval time, while C o a , is the mass per volume ratio of adsorbent versus wastewater (g/L). Total phenol content was determined colorimetrically using the Folin-Ciocalteau reagent (Atanassova et al, 2005;Folin and Ciocalteau, 1927).…”

Section: Methodsmentioning

confidence: 99%