Measuring the damage of heavy metal cadmium in rice seedlings by SRAP analysis combined with physiological and biochemical parameters

Abstract

The SRAP profile and physiological and biochemical parameters could act as appropriate biomarkers for the measurement of Cd contamination during rice production.

Physiological, Biochemical and Molecular Responses of Barley Seedlings to Aluminum Stress

“…The differential band patterns in response to Al stress were likely due to genome rearrangements, point mutations, double chain fractures, DNA insertions/deletions, DNA-protein crosslinking, methylation, and other forms of DNA damage. In comparison with the molecular level of plants affected by heavy metals as reported in previous studies [9,10,19], the polymorphic changes were therefore more significant and reliable, and DNA polymorphisms therefore act as biomarkers for the detection of Al pollution in plants.…”

“…The normal metabolism of oxygen in chloroplasts and mitochondria generates ROS, which is accelerated by the heavy metal content in the soil, and other environmental stresses [19,26]. ROS accumulation leads to lipid peroxidation and increased MDA levels [19,23].…”

“…Homogenates were centrifuged (10000 × g) at 4 o C for 20 min and supernatants were collected for enzymatic analysis. SOD, POD, CAT and Malondialdehyde (MDA) content were measured based on previous studies [19].…”

“…Polymorphisms in the SRAP profiles included the disappearance and appearance of new PCR bands, in comparison to control SRAP profiles. Averages were calculated for each test group exposed to different Al treatments [19]. For the comparison of the sensitivity of each parameter (SOD, CAT, MDA, POD and GTS), changes were calculated as a percentage of their control value (100%).…”

“…Due to the variability of introns, promoters, and intervening sequences across species and individuals, SRAP has utility for comparative genomics, genetic diversity and genetic map construction [18]. Our recent studies have demonstrated the molecular basis of rice DNA damage using SRAP under Cadmium stress [19]. The effects of Al stress in barley plants are less well investigated.…”