Measuring the diffusion coefficient of ganglioside on cell membrane by fluorescence correlation spectroscopy

Dong, Shiqing; You, Minghai; Chen, Jianling; Zhou, Jie; Xie, Shusen; Yang, Hongqin

doi:10.1088/1742-6596/844/1/012047

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Receptor Lipid Diffusion In the Plasma Membrane1

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2020

2023

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Cited by 2 publications

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“…Analysis of the fluorescence signal reveals a diffusion constant of D µkiss = 0.32 ± 0.13 µm 2 s −1 for GM1-CTxB-AF488. FRAP measurements performed on the same sample report D FRAP = 0.35 ± 0.14 µm 2 s −1 , which is in line with previously published values [43][44][45]. The detailed structures in Fig.…”

Section: Receptor Lipid Diffusion In the Plasma Membranesupporting

confidence: 90%

A paintbrush for delivery of nanoparticles and small molecules to live cells with micrometer spatial and millisecond temporal control

Sandoghdar

Holler

Taylor

et al. 2023

Preprint

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Delivery of very small amounts of reagents to cells with micrometer spatial precision and millisecond time resolution is currently out of reach, but would strongly facilitate quantitative studies in cell biology. Here, we present a micropipette-based scheme for brushing a layer of small molecules and nanoparticles onto the live cell membrane from a sub-femtoliter confined volume of a perfusion flow. We characterize our system through both experiment and model and find excellent agreement. We demonstrate several applications that benefit from a controlled brush delivery, such as a direct means to quantify local and long-range membrane mobility and organization as well as dynamical probing of intercellular force signaling.

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Section: Receptor Lipid Diffusion In the Plasma Membranesupporting

confidence: 90%