Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of &lt;em&gt;Caenorhabditis elegans&lt;/em&gt;

Le, Tram Anh Ngoc; Selvaraj, Baskar; Lee, Jae Woo; Kang, Kyungsu

doi:10.3791/60419

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C. elegans, a self-reproducing nematode with a short life span and a transparent body, is a preferred animal model in many APDT studies because of its easy visualization under a microscope and lack of ethical concerns [ 36 , 56 , 57 , 58 , 59 , 60 ]. Our group was the first to document the APDT effects of natural PSs, hypericin, and plant extracts on C. elegans infected with various pathogenic bacteria [ 36 , 56 ].…”

Section: Preclinical and Clinical Apdt Studiesmentioning

confidence: 99%

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

2021

Self Cite

View full text Add to dashboard Cite

Health problems and reduced treatment effectiveness due to antimicrobial resistance have become important global problems and are important factors that negatively affect life expectancy. Antimicrobial photodynamic therapy (APDT) is constantly evolving and can minimize this antimicrobial resistance problem. Reactive oxygen species produced when nontoxic photosensitizers are exposed to light are the main functional components of APDT responsible for microbial destruction; therefore, APDT has a broad spectrum of target pathogens, such as bacteria, fungi, and viruses. Various photosensitizers, including natural extracts, compounds, and their synthetic derivatives, are being investigated. The main limitations, such as weak antimicrobial activity against Gram-negative bacteria, solubility, specificity, and cost, encourage the exploration of new photosensitizer candidates. Many additional methods, such as cell surface engineering, cotreatment with membrane-damaging agents, nanotechnology, computational simulation, and sonodynamic therapy, are also being investigated to develop novel APDT methods with improved properties. In this review, we summarize APDT research, focusing on natural photosensitizers used in in vitro and in vivo experimental models. In addition, we describe the limitations observed for natural photosensitizers and the methods developed to counter those limitations with emerging technologies.

show abstract

Section: Preclinical and Clinical Apdt Studiesmentioning

confidence: 99%

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The reduction in gut leakiness was assessed thought the "smurf assay" which, briefly, is carried out by feeding C. elegans with a dye and observing it though a fluorescent microscope if it passes though the intestinal epithelial barrier. This assay, together with similar ones based on the same principles [61], has also permitted Le et al to characterize the response of C. elegans to different bacteria, including known pathogens and others with probiotic properties like Enterococcus faecalis, which did not affect the gut permeability when compared to pathogens.…”

Section: Probiotics With Anti-inflammatory Properties In C Elegansmentioning

confidence: 99%

Caenorhabditis elegans as a Screening Model for Probiotics with Properties against Metabolic Syndrome

Goyache,

Yavorov-Dayliev,

Milagro

et al. 2024

IJMS

View full text Add to dashboard Cite

There is a growing need to develop new approaches to prevent and treat diseases related to metabolic syndromes, including obesity or type 2 diabetes, that focus on the different factors involved in the pathogenesis of these diseases. Due to the role of gut microbiota in the regulation of glucose and insulin homeostasis, probiotics with beneficial properties have emerged as an alternative therapeutic tool to ameliorate metabolic diseases-related disturbances, including fat excess or inflammation. In the last few years, different strains of bacteria, mainly lactic acid bacteria (LAB) and species from the genus Bifidobacterium, have emerged as potential probiotics due to their anti-obesogenic and/or anti-diabetic properties. However, in vivo studies are needed to demonstrate the mechanisms involved in these probiotic features. In this context, Caenorhabditis elegans has emerged as a very powerful simple in vivo model to study the physiological and molecular effects of probiotics with potential applications regarding the different pathologies of metabolic syndrome. This review aims to summarize the main studies describing anti-obesogenic, anti-diabetic, or anti-inflammatory properties of probiotics using C. elegans as an in vivo research model, as well as providing a description of the molecular mechanisms involved in these activities.

show abstract

Antimicrobial photodynamic therapy with Ligularia fischeri against methicillin-resistant Staphylococcus aureus infection in Caenorhabditis elegans model

Hwang

Alam

et al. 2023

Appl Biol Chem

View full text Add to dashboard Cite

The high prevalence of methicillin-resistant Staphylococcus aureus (MRSA) infection threatens the effectiveness of current clinical settings. Antimicrobial photodynamic therapy (APDT) is a promising alternative to antibiotics for treating infections due to its low resistance. This study aimed to evaluate the antibacterial properties of APDT with L. fischeri extract (LFE) against MRSA and various skin and oral pathogens in vitro and its photopharmaceutical actions in Caenorhabditis elegans. The antimicrobial activities of APDT with LFE against pathogens were evaluated using plate counting method. The chemical profile was characterized using high-performance liquid chromatography and spectrophotometry. The growth rate assay, lifespan assay, and bacterial attachment on worms were performed to assess the therapeutics effects in C. elegans. The swab method was used for the detection of pathogens on the micropig skin surface. The APDT treatment with L. fischeri extract (LFE, 20 µg/mL) and red light (intensity of 120 W/m2) reduced 4.3–4.9 log (colony forming unit/mL) of Staphylococcus aureus, MRSA, Cutibacterium acnes, Streptococcus mutans; and 2.4 log (CFU/mL) of Candida albicans. Chemical analysis revealed that LFE enriched three active photosensitizers. APDT reduced bacterial populations on worms, recovered growth retardation, and improved lifespan in MRSA-infected C. elegans without causing severe side effects. The surface eradication of MRSA after exposure to LFE with red light was demonstrated on micropig skin. These findings highlight the significance of L. fischeri as a natural resource for the safe phototreatment of MRSA infection in the biomedical and cosmeceutical industries.

show abstract

Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of <em>Caenorhabditis elegans</em>

Cited by 3 publications

References 0 publications

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

Natural Photosensitizers in Antimicrobial Photodynamic Therapy

Caenorhabditis elegans as a Screening Model for Probiotics with Properties against Metabolic Syndrome

Antimicrobial photodynamic therapy with Ligularia fischeri against methicillin-resistant Staphylococcus aureus infection in Caenorhabditis elegans model

Contact Info

Product

Resources

About