Measuring the primary production rates of individual phytoplankton species in natural mixed populations

Watt, W. D.

doi:10.1016/0011-7471(71)90038-6

Cited by 28 publications

(28 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relative grain counts of phytoplankton species as a measure of their metabolic activity have been used previously (Brock and Brock, 1968;Fuhs and Canelli, 1970;Maguire and Neill, 1971;Watt, 1971). However, quantitative measurements from autoradiograms are difficult to obtain.…”

Section: Discussionmentioning

confidence: 99%

“…Our pre-1972;Jones, 1972;Faust and Correll, vious results have also indicated that 1976). Bacteria are also opportunists, utilizing photosynthetic products of phytoplankton efficiently (Watt, 1971). Also, algal heterotrophy may exist in nature (Ketchum, 1939;Faust and Gantt, 1973;Pollinger and Berman, 1976).…”

Section: Discussionmentioning

confidence: 99%

“…After exposure, the emulsion was developed in Dektol developer at 15~ for 2 min, followed by a 10 sec distilled water rinse, 5 min in acid fixer and 5 to 10 min washing in running tap water. After drying, the emulsion was cleared for I h in methylsalicylate and mounted in Permount with a cover slip (Watt, 1971). Some slides were dipped, after development but before washing, in 0.05% aqueous safranin for 30 sec.…”

Section: Water Chemistrymentioning

confidence: 99%

See 2 more Smart Citations

Autoradiographic study to detect metabolically active phytoplankton and bacteria in the Rhode river estuary

Faust

Correll

1977

Mar. Biol.

View full text Add to dashboard Cite

The rate of utilization of inorganic carbon (C) and phosphate (P) by phytoplankton and bacteria in the Rhode River estuary has been estimated using liquid-emulsion autoradiography during four times of the year. Metabolic activity of phytoplankton was estimated by calculating silver grains above individual species. From these counts, the relative C and P uptake rates of individual species per unit of biomass and per volume of water were estimated. Examination of the autoradiograms showed that the metabolically most active algae were smaller than 10 ~m. Both C and P were taken up by these smaller species at a higher rate than their proportion of the total biomass per volume of water would indicate. Uptake of C and P per unit of cell volume varied within a species and among the various phytoplankton at the different seasons of the year. Metabolic activity of planktonic bacteria in safranin-stained autoradiograms was also estimated by counting bacteria with associated grains and cells without grains. The ratio of 33p-labeled to unlabeled bacteria was highest in November. This high metabolic activity of bacteria in November corresponded with high P uptake rates of the phytoplankton at that time. Throughout this study, only 28 to 42% of the total phytoplankton biomass was metabolically active and 63 to 85% of the bacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Water Chemistrymentioning

confidence: 99%

See 1 more Smart Citation

Autoradiographic study to detect metabolically active phytoplankton and bacteria in the Rhode river estuary

Faust

Correll

1977

Mar. Biol.

View full text Add to dashboard Cite

show abstract

“…Fixed slides were gently washed in tap water for 5 min, followed by a 30 s dip in deionised water, and air drying overnight. Stull (unpublished 1971), Watt (1971), andPaerl (unpublished 1973) can be consulted for exact equipment requirements or processing. Properly exposed and developed autoradiographs should reveal small (<0.1 /an diameter) silver grains deposited directly over and within a 5-10 ^m radius of radioactive organisms.…”

Section: Autoradiographymentioning

confidence: 99%

Effectiveness of various counting methods in detecting viable phytoplankton

Paerl¹

1978

New Zealand Journal of Marine and Freshwater Research

View full text Add to dashboard Cite

Comparisons of counts from 14 C microautoradiographs of photosynthetically active natural phytoplankton populations with counts from Utermöhl or membrane-filter methods often reveal significant discrepancies in biomass estimates; the viability of armoured species such as diatoms cannot be determined effectively by the latter two methods. Furthermore, nanoplankton < 5 μm in diameter are difficult to distinguish from detrital material, especially when Utermöhl methods are used at routine magnifications. Considering that nanoplankton commonly account for between 20% and 50% of total phytoplankton biomass in various lakes, current methods tend to overlook a major segment of the primary producers. In overestimating viable diatoms, dinoflagellates, and armoured green algae by as much as 50% and underestimating nanoplankton by a similar magnitude, conventional counting methods may be inadequate to describe true phytoplankton biomass. These discrepancies can be discovered and corrected by applying microautoradiography.

show abstract

“…Packard and Dortch (1975) The validity of such exptrapolations is dependent on the constancy of the relative biochemical composition of living organic matter (e.g. Steele and Baird, 1962;Donagbay et al, 1978;Karl, 1980;Goldman, 1980), and independence of the behaviour of different organic reservoirs over time (Banse 1977 Watt, 1971;Maguire and Neill, 1971;Gutel'makher. 1973;Davenport and Maguire, 1984).…”

Section: List Of Tables (Continued)mentioning

confidence: 99%

Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter

Lohrenz¹

1985

View full text Add to dashboard Cite

Measuring the primary production rates of individual phytoplankton species in natural mixed populations

Cited by 28 publications

References 12 publications

Autoradiographic study to detect metabolically active phytoplankton and bacteria in the Rhode river estuary

Autoradiographic study to detect metabolically active phytoplankton and bacteria in the Rhode river estuary

Effectiveness of various counting methods in detecting viable phytoplankton

Primary production of particulate protein amino acids : algal protein metabolism and its relationship to the composition of particulate organic matter

Contact Info

Product

Resources

About