Measuring the Sensitivity of an Indirect Predator Gut Content ELISA: Detectability of Prey Remains in Relation to Predator Species, Temperature, Time, and Meal Size

Hagler, James R.; Naranjo, Steven E.

doi:10.1006/bcon.1997.0521

Cited by 73 publications

(60 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, long detection periods could obscure the frequency of predatory events in the field, since recent feeding events are not distinguishable from older ones, and could lead to overestimation of predation rates. Relatively short detection periods, like the ones found for the other predator-prey combinations in this study, facilitate a clearer interpretation of field data (Hagler & Naranjo, 1997;Sheppard & Harwood, 2005).…”

Section: Discussionsupporting

confidence: 67%

Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids

Ingels¹,

Aebi²,

Hautier³

et al. 2013

Eur. J. Entomol.

View full text Add to dashboard Cite

Abstract. Several studies have demonstrated that the invasive ladybird Harmonia axyridis is a strong intra-guild predator of native species of ladybird. Laboratory studies have shown that H. axyridis can be an intra-guild predator of aphid predators other than coccinellids, including the hoverfly Episyrphus balteatus and lacewing Chrysoperla carnea. However, little is known about the effect of intra-guild predation (IGP) by H. axyridis on hoverfly and lacewing populations in the field. In the present study molecular analyses were used to detect the DNA of E. balteatus and C. carnea in the gut contents of H. axyridis. Primers for the syrphid and chrysopid prey were designed and feeding experiments performed to determine how long prey DNA remains detectable in the guts of this ladybird. DNA detection was influenced by the life stage of the predator and species of prey. Meal size did not affect detection time, except when fourth instar individuals of H. axyridis were fed 10 eggs or one second instar of C. carnea. Predator weight, sex and morpho-type (melanic/non-melanic) did not influence DNA detection. The half-life of the time for which the DNA of the prey remained detectable was calculated for each predator-prey combination, and ranged from 8.9 to 52.4 h. This method can be used to study the ecological importance of IGP by H. axyridis on aphidophagous predators other than coccinellids in the field.

show abstract

Section: Discussionsupporting

confidence: 67%

Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids

Ingels¹,

Aebi²,

Hautier³

et al. 2013

Eur. J. Entomol.

View full text Add to dashboard Cite

show abstract

“…The temporal window of opportunity for detecting the ingested egg protein was no longer than 10 h. The indirect ELISA is extremely sensitive, but the sensitivity depends on the size of the prey (available epitopes for reaction), the rate of digestion of the protein (epitope degradation) by the predator, the temperature at which the predators are held, and ratio of nonreactive proteins that compete for space on the microplate well (Hagler and Naranjo 1997). The temporal window for detection of the prey speciÞc protein may be shorter with sucking predators that partially digest their food before ingesting it (Kiritani andDempster 1973, Cohen 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Different prey species can affect the rate of degradation (Lö vei et al 1990). Other studies indicate that the rate of degradation can change depending on predator species, temperature, time, and meal size (Fichter and Stephen 1981, Hagler and Naranjo 1997, Agustṍ et al 1999. The time that the antigen could be detected in Orius spp.…”

Section: Discussionmentioning

confidence: 99%

Identifying Predation ofHelicoverpa zea(Lepidoptera: Noctuidae) Eggs byOriusspp. (Hemiptera: Anthocoridae) in Cotton by Using Elisa

Sansone

Smith

2001

Environ Entomol

View full text Add to dashboard Cite

“…Predators serving as negative controls were placed live into Petri dishes containing only water for 3 days. This time frame ensures that any whitefly prey remains present at the time of collection were excreted prior to their analyses by the ELISA (Hagler & Naranjo 1997). Negative control predators (n 0/8 Á/16 per ELISA microplate) were assayed by ELISA for each predator species alongside their field-collected counterparts.…”

Section: Methodsmentioning

confidence: 99%

Use of a gut content ELISA to detect whitefly predator feeding activity after field exposure to different insecticide treatments

Hagler

Naranjo

2005

Biocontrol Science and Technology

Self Cite

View full text Add to dashboard Cite

A 2-year commercial-scale study was conducted to qualitatively evaluate the effect of different insecticide treatment regimes on the predator complex attacking Bemisia tabaci (Gennadius) in cotton. In 1996 three insecticide regimes were compared: a rotation of conventional broadspectrum insecticides or one of two different regimes based on the initial use of the insect growth regulators (IGRs), buprofezin and pyriproxyfen. In 1997 the same three regimes plus an untreated control were compared; split-plots were sprayed once for Lygus hesperus Knight control using a broad-spectrum insecticide. Relative feeding activity for each predator species was compared between treatment regimes by analyzing the gut contents of predators for the presence of whitefly remains using a whitefly-specific enzyme-linked immunosorbent assay (ELISA). The ELISA results were combined with predator density data to obtain a qualitative pesticide impact index for each predator group. In total, we analyzed the gut contents of 32 262 field-collected predators, representing nine different taxa. Of these, Pseudatomocelis seriatus (Reuter), Spanagonicus albofasciatus (Reuter), and spiders consisting primarily of Misumenops celer (Hentz) are shown to be whitefly predators for the first time. Predator populations were usually reduced in plots that received applications of broad-spectrum insecticides for B. tabaci and L. hesperus control, but there were few treatment differences in the proportions of predators containing whitefly remains in their guts. However, the feeding activity of certain predator species in fields sprayed with broad spectrum insecticides was significantly reduced compared with those in IGR-based and control treatments. Overall, insecticide regimes using IGRs were less lethal to the whitefly predator complex than regimes consisting of only conventional, broadspectrum insecticides, but differences in predator feeding activity on whitefly between the various insecticide treatment regimes were minimal.

show abstract

Measuring the Sensitivity of an Indirect Predator Gut Content ELISA: Detectability of Prey Remains in Relation to Predator Species, Temperature, Time, and Meal Size

Cited by 73 publications

References 34 publications

Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids

Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids

Identifying Predation ofHelicoverpa zea(Lepidoptera: Noctuidae) Eggs byOriusspp. (Hemiptera: Anthocoridae) in Cotton by Using Elisa

Use of a gut content ELISA to detect whitefly predator feeding activity after field exposure to different insecticide treatments

Contact Info

Product

Resources

About