Research works on the improvement of mare pregnancy diagnostics and evaluation of biological activity of gonadotropic preparation based on the use of enzyme-linked immunosorbent assay were carried out. As the result of diagnostics and evaluation, effective producers as donors for obtaining gonadotropic preparation among the Kazakh population of mares with type "zhabe", the animal units of 60 days with their 5-6 year pregnancy containing FSH -10,380,65 mIU/1 mL and 7-8-year-old with FSH content -10,500,32 mIU/1 mL; 70 days of their 3-4-year-old pregnancy with FSH content -10,520,21 mIU/1 mL, 5-6-year-old with FSH content -11,340,45 mIU/1mL, 7-8-year-old with FSH content -11,340,32 mIU/1 mL, 9-10-year-old with FSH content -11,060,29 mIU/1 mL are identified. A new method for the selection of a drug by biological activity based on the content of follicle-stimulating hormone level for a given herd was developed, which allows to determination of the activity in international units, without the traditional, labor-consuming, outdated method for determination using laboratory white mice, addressing ethical issues in research.