Meat quality is associated with muscle metabolic status but not contractile myofiber type composition in premature pigs

Li, X.; Yang, Xia; Shan, Baosen; Shi, Jincheng; Xia, Dong; Wegner, J.; Zhao, Ruqian

doi:10.1016/j.meatsci.2008.07.022

Cited by 25 publications

(18 citation statements)

References 39 publications

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“…Previous studies demonstrate striking breed differences in plasma cortisol concentration and GR mRNA expression in pigs [16], [17], Chinese indigenous Erhualian (EHL) pigs demonstrate 2-fold higher plasma cortisol concentration than Pietrain pigs [18]. Moreover, GR expression in hippocampus [17], liver and muscle [16] also differs between Chinese and Western pig breeds, which is associated with the breed-specific characteristics in stress responses, hepatic gluconeogenesis, and intramuscular fat deposition, respectively. Accumulating evidence suggests that dysfunction of hepatic GR is involved in the development of metabolic diseases including obesity, diabetes and fatty liver [19], [20].…”

Section: Introductionmentioning

confidence: 98%

“…Pigs serve as excellent model for human metabolic research because they share high similarity to humans in anatomy, physiology, development, metabolism, and omnivorous habits [15]. Previous studies demonstrate striking breed differences in plasma cortisol concentration and GR mRNA expression in pigs [16], [17], Chinese indigenous Erhualian (EHL) pigs demonstrate 2-fold higher plasma cortisol concentration than Pietrain pigs [18]. Moreover, GR expression in hippocampus [17], liver and muscle [16] also differs between Chinese and Western pig breeds, which is associated with the breed-specific characteristics in stress responses, hepatic gluconeogenesis, and intramuscular fat deposition, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Breed-Dependent Transcriptional Regulation of 5′-Untranslated GR (NR3C1) Exon 1 mRNA Variants in the Liver of Newborn Piglets

Zou

Jia

et al. 2012

PLoS ONE

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Glucocorticoids are vital for life and regulate an array of physiological functions by binding to the ubiquitously expressed glucocorticoid receptor (GR, also known as NR3C1). Previous studies demonstrate striking breed differences in plasma cortisol levels in pigs. However, investigation into the breed-dependent GR transcriptional regulation is hampered by lacking porcine GR promoter information. In this study, we sequenced 5.3 kb upstream of the translation start codon of the porcine GR gene, and identified seven alternative 5′-untranslated exons 1–4, 1–5, 1–6, 1–7, 1–8, 1–9,10 and 1–11. Among all these mRNA variants, exons 1–4 and 1–5, as well as the total GR were expressed significantly (P<0.05) higher in the liver of newborn piglets of Large White (LW) compared with Erhualian, a Chinese indigenous breed. Overall level of CpG methylation in the region flanking exons 1–4 and 1–5 did not show breed difference. However, nuclear content of Sp1, p-CREB and GR in the liver was significantly (P<0.05) higher in LW piglets, associated with enhanced binding of p-CREB, and higher level of histone H3 acetylation in 1–4 and 1–5 promoters. In contrast, GR binding to promoters of exons 1–4 and 1–5 was significantly diminished in LW piglets, implicating the presence of negative GREs. These results indicate that the difference in the hepatic expression of GR transcript variants between two breeds of pigs is determined, at least partly, by the disparity in the binding of transcription factors and the enrichment of histone H3 acetylation to the promoters.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Breed-Dependent Transcriptional Regulation of 5′-Untranslated GR (NR3C1) Exon 1 mRNA Variants in the Liver of Newborn Piglets

Zou

Jia

et al. 2012

PLoS ONE

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“…From this expression level, type IIb was assumed to be 1, and the relative ratios of types I, IIa and IIx to IIb were calculated as the corresponding expression of type I, IIa and IIx fibres. The muscle fibre percentage was then calculated from these proportions for each muscle type (Li et al, 2009). In total, 30 MC pigs were used for muscle fibre typing.…”

Section: Methodsmentioning

confidence: 99%

Analysis of troponin I gene polymorphisms and meat quality in Mongcai pigs

Ngu¹,

Nhan²

2012

SA J. An. Sci.

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Troponin I is one of myofibrillar proteins required for the calcium regulation of skeletal muscle contraction. The expression of both genes, TNNI1 and TNNI2, in troponin is muscle fibre specific and may affect meat quality traits. In this study, the PCR-RFLP method was applied to genotype 120 Mongcai pigs at three single-nucleotide polymorphisms (SNPs), namely T5174C, C8350A for TNNI1 (EU743939) and C1167T for TNNI2 (EU696779). The two SNPs, T5174C and C1167T, were significantly associated with drip loss 48 (measured after 48 h) and compression force, while the second TNNI1 SNP (C8350A) did not show any association with meat quality traits. The results also revealed a relationship between the proportion of IIx muscle fibre and TNNI2. Additional analysis showed a significant association of the TNNI2 SNP (C1167T) with blood glucose. Animals carrying the CT genotype had the highest glucose concentration (93.7 mg/dL). The results demonstrate that TNNI1 and TNNI2 are likely to play a relatively important role in the development of pork quality in Mongcai pigs.

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“…Different breeds of pigs demonstrate distinct phenotypes in growth, metabolism, as well as stress-coping characteristics (Cassady, 2007), which is associated with dramatic difference in serum cortisol concentrations (Desautes et al, 1999). Previous studies described the breed-dependent pattern of total GR expression in hippocampus (Wei et al, 2010), liver and muscle (Li et al, 2009) in pigs showing different stress coping style, hepatic gluconeogenesis, and intramuscular fat deposition, respectively. Recently, we cloned a 5.3 kb proximal promoter sequence of porcine GR gene, and identified seven un-translated alternative first exons each preceded by a distinct promoter (Zou et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Breed-specific expression of GR exon 1 mRNA variants and profile of GR promoter CpG methylation in the hippocampus of newborn piglets

Sun

Jia

et al. 2014

Animal

Self Cite

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Glucocorticoid receptor (GR) transcription is driven by alternative promoters to produce different exon 1 mRNA variants. CpG methylation on GR promoters profoundly affects GR transcription. GR in hippocampus is critical for energy homeostasis and stress responses, yet it remains unclear whether hippocampal expression of GR exon 1 mRNA variants and the methylation status of GR promoters differ between Large White (LW) and Erhualian (EHL) pigs showing distinct metabolic and stress-coping characteristics. EHL pigs had higher hippocampus weight relative to BW ( P < 0.01), which was associated with higher serum cortisol level compared with LW pigs. Hippocampal expression of brain-derived neurotrophic factor ( P < 0.05) was significantly higher, while Bax, a pro-apoptotic gene, was significantly lower in EHL pigs ( P < 0.05). Hippocampal expression of total GR did not differ between breeds, yet GR exon 1 to 11 mRNA was significantly higher ( P < 0.01) in EHL pigs, which was associated with a trend of increase ( P = 0.057) in GR protein content. No significant breed difference was detected for the methylation status across the whole region of the proximal GR promoter, while CpG334 and CpG266.267 were differentially methylated, in a reversed manner, between breeds. The methylation status of CpGs 248, 259, 260, 268 and 271 was negatively correlated ( P < 0.05) with GR exon 1 to 11 mRNA abundance. Our results provide fundamental information on the breed-specific characteristics of GR and its mRNA variants expression and the status of DNA methylation on the proximal GR promoter in the pig hippocampus.

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Meat quality is associated with muscle metabolic status but not contractile myofiber type composition in premature pigs

Cited by 25 publications

References 39 publications

Breed-Dependent Transcriptional Regulation of 5′-Untranslated GR (NR3C1) Exon 1 mRNA Variants in the Liver of Newborn Piglets

Breed-Dependent Transcriptional Regulation of 5′-Untranslated GR (NR3C1) Exon 1 mRNA Variants in the Liver of Newborn Piglets

Analysis of troponin I gene polymorphisms and meat quality in Mongcai pigs

Breed-specific expression of GR exon 1 mRNA variants and profile of GR promoter CpG methylation in the hippocampus of newborn piglets

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