mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia

Ghaznavi‐Rad, Ehsanollah; Goering, Richard V.; Shamsudin, Mariana Nor; Weng, Poh Leng; Tavakol, Mehri; Belkum, Alex van; Neela, Vasanthakumari

doi:10.1007/s10096-011-1230-1

Cited by 11 publications

(6 citation statements)

References 13 publications

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“…These findings may be due to the fact that the dru types dt8 h and dt8i might have been entered to the study setting in the last trimester or might have been emerged as a result of the polymorphism of dt10ao dru type. Unlike our results, a study in Malaysia reported nine dru types in SCC mec type III, the most prevalent of which was the dt13d dru type (41%) [17]. Moreover, a study in Scotland detected 25 dru types and 33 dru repeats, with the dt10a and dt7c as the most prevalent dru types, respectively [16].…”

Section: Discussioncontrasting

confidence: 97%

“…The sequencing of this region can be used for detecting and subtyping methicillin-resistant S. aureus (MRSA) [15, 16]. A study reported that the dru typing of ST239 MRSA isolates provided the clearest distinction between SCC mec IIIA and III isolates [17]. Dru types are stable enough and hence, can be used in epidemiological analyses [16, 18].…”

Section: Introductionmentioning

confidence: 99%

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High prevalence of direct repeat unit types of 10di, 8 h and 8i among methicillin resistant Staphylococcus aureus strains with staphylococcal cassette chromosome mec type IIIA isolated in Tehran, Iran

Motallebi

Jabalameli

Beigverdi

et al. 2019

Antimicrob Resist Infect Control

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Background The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is a main concern in burn care centers worldwide. The some reports of MRSA in Iran suggested that MRSA with type SCC mec III is common among burn patients. The aim of this study was to determine the prevalence, virulence genes, and antimicrobial susceptibility of the direct repeat units ( dru ) types of MRSA with SCC mec IIIA isolated from burn wounds in a burn care center in Tehran, Iran. Methods In total, 165 S. aureus isolates were collected from clinical samples. In order to detect MRSA isolates, the mec A gene was amplified through the polymerase chain reaction (PCR) method. Antimicrobial susceptibility was tested using the disc agar diffusion test. Moreover, the PCR method was applied to determine SCC mec types, virulence genes, and antimicrobial resistance genes. The dru region was sequenced and thereby, dru types and dru repeats were identified. A similarity matrix was used to create minimum spanning tree (MST). Results The prevalence of MRSA was 69% (114 out of 165 isolates). Most of MRSA isolates (61 out of 114, 53.5%) were SCC mec type IIIA. All MRSA isolates were vancomycin-susceptible and more than 68% of MRSA isolates with SCC mec type IIIA were mupirocin resistant. The successful dru typing of isolates with SCC mec type IIIA revealed fourteen different dru types. There were two new dru types, namely dt10di and dt7aj. MST analysis indicated the presence of the three clusters of dt10di (cluster I), dt8i-dt8 h (cluster II), and dt11c-dt10ao-dt11dd-dt11a-dt10a (cluster III). There were significant differences between clusters I and II respecting antimicrobial resistance pattern and virulence genes. Conclusion Three main dru clusters are prevalent in the study setting. The main dru types in the setting are dt10di, dt8i, and dt8 h. Dru typing can be used to differentiate MRSA strains with SCC mec IIIA.

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Section: Discussioncontrasting

confidence: 97%

Section: Introductionmentioning

confidence: 99%

High prevalence of direct repeat unit types of 10di, 8 h and 8i among methicillin resistant Staphylococcus aureus strains with staphylococcal cassette chromosome mec type IIIA isolated in Tehran, Iran

Motallebi

Jabalameli

Beigverdi

et al. 2019

Antimicrob Resist Infect Control

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“…The remaining 7 dru types (dt13d, dt13g, dt14d, dt14i, dt10h, dt11a, and dt11c) were found in SCCmec type III strains. Among the 7 dru types, 3 (dt13g, dt13d, and dt14d) were also detected in MRSA strains isolated from 2 tertiary hospitals in Kuala Lumpur (18,38). Three dru types (dt14i, dt10h, and dt11c), including one novel dru type (dt11c), have never been reported in Malaysia.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

et al. 2012

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SUMMARY: Staphylococcus aureus is a persistent human pathogen responsible for a variety of infections ranging from soft-tissue infections to bacteremia. The objective of this study was to determine genetic relatedness between methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. We isolated 35 MRSA and 21 MSSA strains from sporadic cases at the main tertiary hospital in Terengganu, Malaysia, screening them for the presence of virulence genes. Their genetic relatedness was determined by accessory gene regulator (agr) types, PCR-restriction fragment length polymorphism (RFLP) of the coa gene, pulsed-field gel electrophoresis (PFGE), S. aureus protein A (spa), and multilocus-sequence typing (MLST). We found that 57z of MRSA and 43z of MSSA strains harbored enterotoxin genes. The majority (87.5z) of the strains were agr type I. PCR-RFLP and PFGE genotyping of the coa gene revealed that MRSA strains were genetically related, whereas MSSA strains had higher heterogeneity. The combined genotype, MLST-spa type ST239-t037, was shared among MRSA and MSSA strains, indicating that MRSA strains could have evolved from MSSA strains. Two combined MLST-spa types were present in MRSA strains, whereas 7 different MLST-spa types were detected in MSSA strains, including 2 combined types (ST779-t878 and ST1179-t267) that have not been reported in Malaysia. In conclusion, enterotoxin genes were more prevalent in MRSA than in MSSA strains in the Terengganu hospital. The MSSA strains were genetically more diverse than the MRSA strains.

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“…Several molecular biology techniques like multi-locus sequence typing (MLST), pulse-field gel electrophoresis (PFGE), SCC mec typing, and spa -typing have been used to study epidemiology and clonal diversity of S. aureus (Maslow et al, 1993; Norazah et al, 2001; Ghaznavi-Rad et al, 2011). However, not a single technique alone could discriminate the bacteria because of differences in the degree of typeability, reproducibility, and discriminatory power (Tenover et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Susceptibility, Virulence Pattern, and Typing of Staphylococcus aureus Strains Isolated From Variety of Infections in India

et al. 2019

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Staphylococcus aureus is one of the major causes of nosocomial infections. This organism produces powerful toxins and cause superficial lesions, systemic infections, and several toxemic syndromes. A total of 109 S. aureus strains isolated from a variety of infections like ocular diseases, wound infection, and sputum were included in the study. Minimum inhibitory concentration (MIC) was determined against 8 antimicrobials. PCR determined the presence of 16S rRNA, nuc, mecA, czrC, qacA/B, pvl, and toxin genes in S. aureus isolates. Pulse-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), SCCmec, spa-, and agr-typing and serotyping determined the diversity among them. All isolates of S. aureus were resistant to two or more than two antibiotics and generated 32 resistance patterns. These isolates were positive for 16S rRNA and S. aureus-specific nuc gene, but showed variable results for mecA, czrC, and qacA/B and pvl genes. Of the 32 methicillin-resistant S. aureus (MRSA), 13 strains carried SCCmec type V, seven type IV, two type III, and nine carried unreported type UT6. Of the 109 strains, 98.2% were positive for hlg, 94.5% for hla, 86.2% for sei, 73.3% for efb, 70.6% for cna, 30.2% for sea, and 12.8% for sec genes. Serotypes VII and VI were prevalent among S. aureus strains. PFGE analysis grouped the 109 strains into 77 clusters. MLST classified the strains into 33 sequence types (ST) and eight clonal complexes (CCs) of which 12 were singletons, and two belong to new allelic profiles. Isolates showed 46 spa-types that included two new spa-types designated as t14911 and t14912. MRSA and methicillin-susceptible S. aureus (MSSA) isolates were diverse in terms of antibiotic resistance pattern, toxin genotypes, SCCmec types, serotypes and PFGE, MLST, and spa-types. However, few isolates from eye infection and wound infection belong to CC239, ST239, and spa-type t037/t657. The study thus suggests that S. aureus strains are multidrug resistant, virulent, and diverse irrespective of sources and place of isolation. These findings necessitate the continuous surveillance of multidrug-resistant and virulent S. aureus and monitoring of the transmission of infection.

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mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia

Cited by 11 publications

References 13 publications

High prevalence of direct repeat unit types of 10di, 8 h and 8i among methicillin resistant Staphylococcus aureus strains with staphylococcal cassette chromosome mec type IIIA isolated in Tehran, Iran

High prevalence of direct repeat unit types of 10di, 8 h and 8i among methicillin resistant Staphylococcus aureus strains with staphylococcal cassette chromosome mec type IIIA isolated in Tehran, Iran

Comparison of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains Isolated from a Tertiary Hospital in Terengganu, Malaysia

Antibiotic Susceptibility, Virulence Pattern, and Typing of Staphylococcus aureus Strains Isolated From Variety of Infections in India

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