mecA positive Staphylococcus spp. in bovine mastitis, milkers, milking environment, and the circulation of different MRSA clones at dairy cows farms in the Northeast region of Brazil

Abstract

This study detected the presence and distribution of mecA in Staphylococcus spp. in the dairy production environment at farm level in Brazil. We analyzed 335 samples of mastitis cow milk, 15 samples of nostrils and hand swabs from milkers, 14 teat cup swabs, and 9 milking buckets swabs. Initially, the samples were subjected to microbiological analysis to detect Staphylococcus spp. and then S. aureus and mecA positive isolates were identified by PCR. All S. aureus isolates carrying the mecA genes were subjected to DNA macro-restriction analysis by Pulsed-Field Gel Electrophoresis (PFGE). The mecA gene was detected in 6/335 (1.78%) of mastitis cow milk, 5/15 (33.3%), and 5/15 (33.3%) of nostrils and hand swab, and 4/14 (28.5%) of the teat cup isolates. MRSA genotyping was performed by PFGE, a total of seven pulsotypes were grouped in two clusters. This study identified the occurrence and spread of MRSA at dairy environment of farms, and also the existence of distinct genetic profiles between isolates.

Effect of Sonication Associated with Pasteurization on the Inactivation of Methicillin-Resistant Staphylococcus aureus in Milk Cream

“…Five strains of mecA-positive β-lactam-resistant Staphylococcus aureus (MRSA) associated with livestock activities in Brazilian territory were used in this study. Specifically, strains 30PD.1 and 32AD.1 were isolated from cow milk, strain 3N originated from a milker's nasal cavity, and strains 1T.1 and SFT.1 were isolated from cow teats [12].…”

Prevalence and Antibiogram of Escherichia coli and Staphylococcus spp. Isolated from Cattle Milk Products Sold in Juja Sub-County, Kenya

“…Bacteria, such as Escherichia coli and Staphylococcus spp., can colonize the udder of cattle leading to the occurrence of mastitis [ 1 , 2 ]. These bacteria are transmitted to the dairy animals from the environment, during the milking process when the milker's hands or milking equipment lack adequate hygiene or along the milk product production chain by contaminated water or utensils used by vendors [ 3 ]. Humans can subsequently consume these bacteria leading to disease.…”