Mechanical alterations of the bone-cartilage unit in a rabbit model of early osteoarthrosis

Pragnere, Sarah; Boulocher, C.; Pollet, Ophélie; Bosser, C; Levillain, Aurélie; Cruel, Magali; Hoc, Thierry

doi:10.1016/j.jmbbm.2018.03.033

Cited by 14 publications

(7 citation statements)

References 62 publications

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“…22,36,37 Second, our study design allows for assessment of contracture duration after remobilization without comorbidities that are associated with destabilized knees, including post-traumatic OA. 22,36,37 The possibility for adding intra-articular injury (i.e. femoral condyle violation) allowed us to investigate the influence of intra-articular bleeding on the fibrotic pathway, which mimic the conventional process after orthopaedic surgery.…”

Section: Discussionmentioning

confidence: 99%

“…We choose to not destabilize the knee joint using ligament disruption, as such techniques commonly induce OA-related synovial fibrosis. 22,36,37 Second, our study design allows for assessment of contracture duration after remobilization without comorbidities that are associated with destabilized knees, including post-traumatic OA. 22,36,37 The possibility for adding intra-articular injury (i.e.…”

Section: Discussionmentioning

confidence: 99%

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Knee immobilization reproduces key arthrofibrotic phenotypes in mice

Dagneaux

Limberg

Owen

et al. 2023

Bone & Joint Research

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Aims As has been shown in larger animal models, knee immobilization can lead to arthrofibrotic phenotypes. Our study included 168 C57BL/6J female mice, with 24 serving as controls, and 144 undergoing a knee procedure to induce a contracture without osteoarthritis (OA). Methods Experimental knees were immobilized for either four weeks (72 mice) or eight weeks (72 mice), followed by a remobilization period of zero weeks (24 mice), two weeks (24 mice), or four weeks (24 mice) after suture removal. Half of the experimental knees also received an intra-articular injury. Biomechanical data were collected to measure passive extension angle (PEA). Histological data measuring area and thickness of posterior and anterior knee capsules were collected from knee sections. Results Experimental knees immobilized for four weeks demonstrated mean PEAs of 141°, 72°, and 79° after zero, two, and four weeks of remobilization (n = 6 per group), respectively. Experimental knees demonstrated reduced PEAs after two weeks (p < 0.001) and four weeks (p < 0.0001) of remobilization compared to controls. Following eight weeks of immobilization, experimental knees exhibited mean PEAs of 82°, 73°, and 72° after zero, two, and four weeks of remobilization, respectively. Histological analysis demonstrated no cartilage degeneration. Similar trends in biomechanical and histological properties were observed when intra-articular violation was introduced. Conclusion This study established a novel mouse model of robust knee contracture without evidence of OA. This was appreciated consistently after eight weeks of immobilization and was irrespective of length of remobilization. As such, this arthrofibrotic model provides opportunities to investigate molecular pathways and therapeutic strategies. Cite this article: Bone Joint Res 2023;12(1):58–71.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Knee immobilization reproduces key arthrofibrotic phenotypes in mice

Dagneaux

Limberg

Owen

et al. 2023

Bone & Joint Research

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“…1 g–i). Altered cartilage moduli indicate OA progression and cartilage erosion in PT-OA [ [43] , [44] , [45] ]. Doyran et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of knee joint loading on chondrocyte mechano-vulnerability and severity of post-traumatic osteoarthritis induced by ACL-injury in mice

Kotelsky

Elahi

Yigit

et al. 2022

Osteoarthritis and Cartilage Open

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“…Subchondral bone undergoes different pathological changes during the development of OA, in which subchondral bone wear occurs in the early stage, while the typical pathological changes in the late stage are subchondral bone sclerosis [91]. A recent study has suggested that the alteration of subchondral bone structure is the main cause of OA in animal models [92]. Fang et al also found that early damage to subchondral bone (reduction of bone volume) occurs prior to cartilage degeneration and osteophyte formation, whereas in the late-stage of OA, this damage presents as subchondral bone sclerosis in mouse models, which is consistent with the typical pathology of patients with OA [91].…”

Section: Subchondral Bonementioning

confidence: 99%

Senescent skeletal cells cross-talk with synovial cells plays a key role in the pathogenesis of osteoarthritis

Liu

Huan

et al. 2022

Arthritis Res Ther

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Osteoarthritis (OA) has been recognized as an age-related degenerative disease commonly seen in the elderly that affects the whole “organ” including cartilage, subchondral bone, synovium, and muscles. An increasing number of studies have suggested that the accumulation of senescent cells triggering by various stresses in the local joint contributes to the pathogenesis of age-related diseases including OA. In this review, we mainly focus on the role of the senescent skeletal cells (chondrocytes, osteoblasts, osteoclasts, osteocyte, and muscle cells) in initiating the development and progression of OA alone or through cross-talk with the macrophages/synovial cells. Accordingly, we summarize the current OA-targeted therapies based on the abovementioned theory, e.g., by eliminating senescent skeletal cells and/or inhibiting the senescence-associated secretory phenotype (SASP) that drives senescence. Furthermore, the existing animal models for the study of OA from the perspective of senescence are highlighted to fill the gap between basic research and clinical applications. Overall, in this review, we systematically assess the current understanding of cellular senescence in OA, which in turn might shed light on the stratified OA treatments.

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Mechanical alterations of the bone-cartilage unit in a rabbit model of early osteoarthrosis

Cited by 14 publications

References 62 publications

Knee immobilization reproduces key arthrofibrotic phenotypes in mice

Knee immobilization reproduces key arthrofibrotic phenotypes in mice

Effect of knee joint loading on chondrocyte mechano-vulnerability and severity of post-traumatic osteoarthritis induced by ACL-injury in mice

Senescent skeletal cells cross-talk with synovial cells plays a key role in the pathogenesis of osteoarthritis

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