Mechanical and failure properties of single attached cells under compression

Peeters, Eag Emiel; Oomens, Cwj Cees; Bouten, Cvc Carlijn; Bader, DL Dan; Baaijens, Fpt Frank

doi:10.1016/j.jbiomech.2004.07.018

Cited by 101 publications

(78 citation statements)

References 35 publications

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“…In our simulations of cell indentation, the endothelial cell is modeled as a nearly incompressible (14,27) hyperelastic neo-Hookean (13,28,29) material, with a Poisson's ratio of 0.49 and a Young's modulus of 1 kPa. The microindenter's spherical tip is considered infinitely rigid compared to the cell.…”

Section: Simulations Of Cell Indentationmentioning

confidence: 99%

“…For strong indentations, the indentation depth reaches a maximum value, Dz ¼ Dz max , corresponding to the cell becoming infinitely rigid when compared to the microindenter. Indeed, the cell will appear progressively stiffer as the indentation increases relative to the sample thickness (14), saturating at indentations of~60% of the cell height, where the cell becomes nearly infinitely rigid (13,38). Our experimental results indicate that the moderate indentation regime is valid up to Dz cutoff zDd cutoff z0:3 mm, corresponding to the initial region of the indentation curve that is well described by Hertz's contact theory (see Supporting Materials and Methods for details), whereas the maximum indentation is estimated to be on the order of Dz max z0:6 mm (see below).…”

Section: Tilted Microindentation Allows Application Of a Controlled Fmentioning

confidence: 99%

“…However, under high stresses, such as those arising during the deployment of a stent, endothelial cells can be wounded beyond repair (10). Plasma membrane rupture under compressive stress has previously been studied in red blood cells using atomic force microscopy (AFM) (11,12) and in mouse myoblasts using a whole-cell compression apparatus (13). However, in those studies, the shape and size of the compressive apparatus was maintained constant, yielding a single critical force value.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mechanical Criterion for the Rupture of a Cell Membrane under Compression

Gonzalez‐Rodriguez

Guillou

Cornat

et al. 2016

Biophysical Journal

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We investigate the mechanical conditions leading to the rupture of the plasma membrane of an endothelial cell subjected to a local, compressive force. Membrane rupture is induced by tilted microindentation, a technique used to perform mechanical measurements on adherent cells. In this technique, the applied force can be deduced from the measured horizontal displacement of a microindenter's tip, as imaged with an inverted microscope and without the need for optical sensors to measure the microindenter's deflection. We show that plasma membrane rupture of endothelial cells occurs at a well-defined value of the applied compressive stress. As a point of reference, we use numerical simulations to estimate the magnitude of the compressive stresses exerted on endothelial cells during the deployment of a stent.

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Section: Simulations Of Cell Indentationmentioning

confidence: 99%

Section: Tilted Microindentation Allows Application Of a Controlled Fmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mechanical Criterion for the Rupture of a Cell Membrane under Compression

Gonzalez‐Rodriguez

Guillou

Cornat

et al. 2016

Biophysical Journal

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“…Peak reaction forces at 70 % compression of 2500 nN are reported for highly contractile myoblasts [34], while lower forces are reported for less contractile cells: 500 nN for endothelial cells [8] and 360 nN for fibroblasts [35]. This trend suggests that highly contractile cells provide a greater resistance to applied compression.…”

Section: Discussionmentioning

confidence: 95%

Experimental and Computational Investigation of the Role of Stress Fiber Contractility in the Resistance of Osteoblasts to Compression

et al. 2013

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Provided by the author(s) and NUI Galway in accordance with publisher policies. Please cite the published version when available. Downloaded 2018-05-09T21:17:08Z Some rights reserved. For more information, please see the item record link above. TitleExperimental and computational investigation of the role of stress fiber contractility in the resistance of osteoblasts to compression AbstractThe mechanical behavior of the actin cytoskeleton has previously been investigated using both experimental and computational techniques. However, these investigations have not elucidated the role the cytoskeleton plays in the compression resistance of cells. The present study combines experimental compression techniques with active modeling of the cell's actin cytoskeleton. A modified atomic force microscope is used to perform whole cell compression of osteoblasts. Compression tests are also performed on cells following the inhibition of the cell actin cytoskeleton using cytochalasin-D. An active bio-chemo-mechanical model is employed to predict the active remodeling of the actin cytoskeleton. The model incorporates the myosin driven contractility of stress fibers via a muscle-like constitutive law. The passive mechanical properties, in parallel with active stress fiber contractility parameters, are determined for osteoblasts. Simulations reveal that the computational framework is capable of predicting changes in cell morphology and increased resistance to cell compression due to the contractility of the actin cytoskeleton. It is demonstrated that osteoblasts are highly contractile and that significant changes to the cell and nucleus geometries occur when stress fiber contractility is removed.

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“…In this case, suitable constitutive equations might be obtained by assuming that the cell is hyperelastic, in which case the stress components can be derived from an appropriate strain energy function. While there are many types of strain energy function that might be chosen for modelling, the neoHookean material model has been used successfully to describe nonlinear elastic behaviour of cells in compression experiments on endothelial cells [18] and eukaryotic cells [19]. However, biological cells were found to be viscoelastic at large deformations, and showed significant force relaxation after compression [11,20].…”

Section: Computational Modelling For Characterisation Of Mechanimentioning

confidence: 99%