Mechanical and morphological response of confluent epithelial cell layers to reinforcement and dissolution of the F-actin cytoskeleton

Brückner, Bastian Rouven; Nöding, Helen; Skamrahl, Mark; Janshoff, Andreas

doi:10.1016/j.pbiomolbio.2018.08.010

Cited by 26 publications

(28 citation statements)

References 69 publications

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“…This crosstalk could be at the origin of some inconsistency in the existing literature. In fact, while an overall stiffening of the cell has been reported [70], consistent with the stabilization effect of the drug, other authors observed the opposite behavior in the past [71]. One of the main advantages of the proposed ES approach is that these intertwined components of cell mechanics can be evaluated separately.…”

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelmentioning

confidence: 55%

Elasticity spectra as a tool to investigate actin cortex mechanics

et al. 2020

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Background The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young’s modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young’s modulus. Conclusions The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

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Section: Characterizing the Actin Cortex With The Bilayer Decay Modelmentioning

confidence: 55%

Elasticity spectra as a tool to investigate actin cortex mechanics

et al. 2020

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“…Based on both observations, we suggest that probably V. parahaemolyticus PMC53.7-Zot contributes to cause redistribution of actin cytoskeleton also described for the V. cholerae -Zot. The effect observed in Caco-2 cells could be explained by differences in the actin distribution, since it is the F-actin cytoskeleton, as well as its connection to the plasma membrane, that is responsible for providing the structure and shape of epithelial cells (Brückner et al, 2019 ). Regrettably, despite all efforts to obtain a PMC53.7-ΔZot strain, it was not possible, even using diverse methodologies.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7

Pérez-Reytor

Pavón

López-Joven

et al. 2020

Front. Cell. Infect. Microbiol.

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Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated.

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“…This crosstalk could be at the origin of some inconsistency in the existing literature. In fact, while an overall stiffening of the cell has been reported [67], consistent with the stabilization effect of the drug, other authors observed the opposite behavior in the past [68]. One of the main advantages of the proposed ES approach is that these intertwined components of cell mechanics can be evaluated separately.…”

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelmentioning

confidence: 55%

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

Preprint

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Background The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young's modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young's modulus. Conclusions The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

show abstract

Mechanical and morphological response of confluent epithelial cell layers to reinforcement and dissolution of the F-actin cytoskeleton

Cited by 26 publications

References 69 publications

Elasticity spectra as a tool to investigate actin cortex mechanics

Elasticity spectra as a tool to investigate actin cortex mechanics

Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

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