Mechanical behavior of a cellulose-reinforced scaffold in vascular tissue engineering

Pooyan, Parisa; Tannenbaum, Rina; Garmestani, Hamid

doi:10.1016/j.jmbbm.2011.09.009

Cited by 98 publications

(54 citation statements)

References 35 publications

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“…CA (M w =30,000, Sigma Aldrich) was dissolved by mixing with a 1:1 mixture of acetone and acetic acid at 15% (w/v) for 24 h. The solution was then loaded into a plastic syringe connected to an 18 gauge needle mounted on a syringe pump (KD Scientific) and electrospun onto a drum collector 17 cm from the needle tip under 17 kV at 1 ml h −1 . Thermal treatment was then conducted as previously described (Wells 2008;Pooyan et al 2012). Briefly, the CA nanofibrous mat was thermally treated in a drying oven at 207°C for 0, 1, and 2 h so that cellular behavior could be observed according to changes in the stiffness of the mat.…”

Section: Methodsmentioning

confidence: 99%

“…For example, cellulose acetate is actively used for bone and cartilage tissue engineering because of its biocompatibility and mechanical strength (Katoh and Urist 1993;Mayer-Wagner et al 2011). In addition, CA has been investigated as a material for vascular tissue and peripheral nervous system recovery (Han and Cheung 2011;Pooyan et al 2012). However, few studies have employed 3D cultures of astrocytes on CA nanofiber.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Min

Jung

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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Astrocytes are involved in neuron protection following central nervous system (CNS) injury; accordingly, engineered astrocytes have been investigated for their usefulness in cell therapy for CNS injury. Nanofibers have attracted a great deal of attention in neural tissue engineering, but their mechanical properties greatly influence physiology. Cellulose acetate (CA) has been studied for use in scaffolds owing to its biocompatibility, biodegradability, and good thermal stability. In this study, stiffness of CA nanofibers controlled by heat treatment was shown to regulate astrocyte activity. Adhesion and viability increased in culture as substrate became stiffer but showed saturation at greater than 2 MPa of tensile strength. Astrocytes became more active in terms of increasing intermediate filament glial fibrillary acidic protein (GFAP). The results of this study demonstrate the effects of stiffness alone on cellular behaviors in a three-dimensional culture and highlight the efficacy of heat-treated CA for astrocyte culture in that the simple treatment enables control of astrocyte activity.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Min

Jung

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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“…An ideal scaffold must be able to provide the essential properties and function to satisfy simultaneously the biological and mechanical requirements for optimal tissue regeneration [13]. To reach these requirements, several studies have been developed based on (i) 3D porous scaffolds with arbitrary architecture (uncontrolled pore size and spatial distribution); (ii) 3D porous scaffolds with hybrid architecture (pore size and spatial distribution partially controlled); and (iii) 3D porous scaffolds with controlled architecture (pore size and spatial distribution) [14].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Morouço

Biscaia

Viana

et al. 2016

BioMed Research International

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Biomaterial properties and controlled architecture of scaffolds are essential features to provide an adequate biological and mechanical support for tissue regeneration, mimicking the ingrowth tissues. In this study, a bioextrusion system was used to produce 3D biodegradable scaffolds with controlled architecture, comprising three types of constructs: (i) poly(ε-caprolactone) (PCL) matrix as reference; (ii) PCL-based matrix reinforced with cellulose nanofibers (CNF); and (iii) PCL-based matrix reinforced with CNF and hydroxyapatite nanoparticles (HANP). The effect of the addition and/or combination of CNF and HANP into the polymeric matrix of PCL was investigated, with the effects of the biomaterial composition on the constructs (morphological, thermal, and mechanical performances) being analysed. Scaffolds were produced using a single lay-down pattern of 0/90°, with the same processing parameters among all constructs being assured. The performed morphological analyses showed a satisfactory distribution of CNF within the polymer matrix and high reliability was obtained among the produced scaffolds. Significant effects on surface wettability and thermal properties were observed, among scaffolds. Regarding the mechanical properties, higher scaffold stiffness in the reinforced scaffolds was obtained. Results from the cytotoxicity assay suggest that all the composite scaffolds presented good biocompatibility. The results of this first study on cellulose and hydroxyapatite reinforced constructs with controlled architecture clearly demonstrate the potential of these 3D composite constructs for cell cultivation with enhanced mechanical properties.

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“…CNCs have recently attracted the interest of researchers in the fields of tissue engineering and regenerative medicine for use as scaffolds or drug-delivery systems. CNCs dispersed within a cellulose acetate propionate (CAP) matrix formed a three-dimensional rigid percolating network, which was contemplated for use as a scaffold in small-diameter vascular grafts [25]. Huang et al [26] prepared electrospun silk fibroin nanofiber mats reinforced with CNCs; the tensile strength and Young's modulus of these mats were almost twice those of unreinforced silk fibroin mats when the CNC content was 2 wt.%.…”

Section: Introductionmentioning

confidence: 99%

Preparation and properties of PLGA nanofiber membranes reinforced with cellulose nanocrystals

Guo

Liu

et al. 2015

Colloids and Surfaces B: Biointerfaces

105

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Mechanical behavior of a cellulose-reinforced scaffold in vascular tissue engineering

Cited by 98 publications

References 35 publications

Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Regulation of astrocyte activity via control over stiffness of cellulose acetate electrospun nanofiber

Fabrication of Poly(ε-caprolactone) Scaffolds Reinforced with Cellulose Nanofibers, with and without the Addition of Hydroxyapatite Nanoparticles

Preparation and properties of PLGA nanofiber membranes reinforced with cellulose nanocrystals

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