The primary purpose of the structural study on the 4.2-meter-long Jaloe Kayoh boat is to analyze the FEA of E-glass and ramie fiber composites, as well as the polyester resin. The hand lay-up method was used in this study to manufacture specimens following the ASTM D638-02 standard, while the tensile test was performed under the ASTM D790-02 standard. Based on the simulation results, the data analysis method was used to visualize the relationship between the stress and strain strength parameters on the structure, as well as a reaction force and displacement, which were related to the centralized loading on the walls and hull of the Jaloe Kayoh boat with a minimum loading variation of 539.3 N and a maximum loading variation of 2157.4 N. The maximum loading of the stress strength on the hull wall of Jaloe Kayoh was obtained at a load of 220 kg or 2157.4 N. The stress values obtained were 4.212e+09 N/mm2 for E-glass fiber and 3.998e+09 N/mm2 for ramie fiber. The highest Reaction Force values obtained were 4.369e+03 N for E-glass and 4.952e+03 N for ramie fiber. The highest displacement value was obtained, which was 2.620e-02 m for E-glass fiber and 2.662e-02 m for ramie fiber. Based on the simulation results, E-glass and ramie fibers are still safe and feasible to use as a base or initial layer, or one of the supporting layers, for lamination on one of the materials for the Jaloe Kayoh boat's walls and hull.