Mechanical, biochemical, and morphological topography of ovine knee cartilage

Risch, Makayla; Easley, Jeremiah T.; McCready, Erin; Troyer, Kevin L.; Johnson, James Weldon; Gadomski, Benjamin C.; McGilvray, Kirk C.; Kisiday, John D.; Nelson, Brad B.

doi:10.1002/jor.24835

Cited by 10 publications

(18 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Testing was performed under force control using a 3-mm hemispherical indenter attached to a 100 N load cell and servohydraulic material testing system (MTS 858, Mini Bionix II, Scotia, NY). 19 Hydration of articular cartilage was maintained during testing using a 400 mOsm preservative saline solution that contained nonspecific protease inhibitors, antibiotics, and antimycotics (5 mM Benzamidine HCl, 5 mM EDTA, Sigma-Aldrich, St. Louis, MO; 1x Antibiotic-Antimycotic, Life Tech, Carlsbad, CA) to minimize autolysis during testing. Each test was performed by placing the actuator perpendicular to the ROI (approximately 2 mm from the surface) and then ramped at a rate of .025 mm/s until a preload of .05 N was achieved.…”

Section: Methodsmentioning

confidence: 99%

“…The preload was held for 100 seconds, after which the load was increased at a rate of .1 N/s to .8 N and allowed to creep for 100 seconds. 19 The recovery curve data (displacement vs. time) were used to calculate percent creep at each ROI. Creep was normalized to the measured displacement during testing at the end of preload to account for variation between samples.…”

Section: Methodsmentioning

confidence: 99%

“…Creep was normalized to the measured displacement during testing at the end of preload to account for variation between samples. 19 Following mechanical testing, an oscillating saw was used to score samples along the template lines into the subchondral bone plate thus physically delineating and enabling accuracy of each ROI for each subsequent analysis.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Biomechanical, Morphological, and Biochemical Characteristics of Articular Cartilage of the Ovine Humeral Head

et al. 2022

Self Cite

View full text Add to dashboard Cite

Objective. Shoulder pain is commonly attributed to rotator cuff injury or osteoarthritis. Ovine translational models are used to investigate novel treatments aimed at remedying these conditions to prevent articular cartilage degeneration and subsequent joint degradation. However, topographical properties of articular cartilage in the ovine shoulder are undefined. This study investigates the biomechanical, morphological, and biochemical attributes of healthy ovine humeral head articular cartilage and characterizes topographical variations between surface locations. Design. Ten humeral heads were collected from healthy skeletally mature sheep and each was segregated into 4 quadrants using 16 regions of interest (ROIs) across the articular surface. Articular cartilage of each ROI was analyzed for creep indentation, thickness, and sulfated glycosaminoglycan (sGAG) and collagen quantity. Comparisons of each variable were made between quadrants and between ROIs within each quadrant. Results. Percent creep, thickness, and sGAG content, but not collagen content, were significantly different between humeral head quadrants. Subregion analysis of the ROIs within each surface quadrant revealed differences in all measured variables within at least one quadrant. Percent creep was correlated with sGAG (r = −0.32, P = 0.0001). Collagen content was correlated with percent creep (r = 0.32, P = 0.0009), sGAG (r = −0.19, P = 0.049), and thickness (r = −0.19, P = 0.04). Conclusions. Topographical variations exist in mechanical, morphologic, and biochemical properties across the articular surface of the ovine humeral head. Recognizing this variability in ovine humeral head cartilage will provide researchers and clinicians with accurate information that could impact study outcomes.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Biomechanical, Morphological, and Biochemical Characteristics of Articular Cartilage of the Ovine Humeral Head

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…We further assessed the capability of Raman biomarkers to predict properties of cartilage from different anatomical sites of the ovine knee joint, which exhibit biochemical and mechanical variability 26 . Raman probe measurements and mechanical testing were performed on osteochondral explants (6.0mm) from the medial and lateral femoral condyles, and tibial plateaus (1-2 explants per surface) of a skeletally mature ovine knee joint.…”

Section: Inter-anatomical Variability As Revealed By Raman Spectroscopy Ex Vivomentioning

confidence: 99%

Raman Needle Arthroscopy for In Vivo Molecular Assessment of Cartilage

Kroupa

Zhang

et al. 2021

Preprint

View full text Add to dashboard Cite

The development of treatments for osteoarthritis (OA) is burdened by the lack of standardized biomarkers of cartilage health that can be applied in clinical trials. We present a novel arthroscopic Raman probe that can optically biopsy cartilage and quantify key ECM biomarkers for determining cartilage composition, structure, and material properties in health and disease. Technological and analytical innovations to optimize Raman analysis include: 1) multivariate decomposition of cartilage Raman spectra into ECM-constituent-specific biomarkers (glycosaminoglycan [GAG], collagen [COL], water [H2O] scores), and 2) multiplexed polarized Raman spectroscopy to quantify superficial zone collagen anisotropy via a PLS-DA-derived Raman collagen alignment factor (RCAF). Raman measurements were performed on a series of ex vivo cartilage models: 1) chemically GAG-depleted bovine cartilage explants (n=40), 2) mechanically abraded bovine cartilage explants (n=30), 3) aging human cartilage explants (n=14), and 4) anatomical-site-varied ovine osteochondral explants (n=6). Derived Raman GAG score biomarkers predicted 95%, 66%, and 96% of the variation in GAG content of GAG-depleted bovine explants, human explants, and ovine explants, respectively (p<0.001). RCAF values were significantly different for explants with abrasion-induced superficial zone collagen loss (p<0.001). The multivariate linear regression of Raman-derived ECM biomarkers (GAG and H2O scores) predicted 94% of the variation in elastic modulus of ovine explants (p<0.001). Finally, we demonstrated the first in vivo Raman arthroscopy assessment of an ovine femoral condyle through intraarticular entry into the synovial capsule. This work advances Raman arthroscopy towards a transformative low cost, minimally invasive diagnostic platform for objective monitoring of treatment outcomes from emerging OA therapies.

show abstract

“…Mechanical indentation (including AFM techniques), performed using either creep or stress-relaxation protocols, is widely employed for assessing the biomechanical behavior of cartilage and OA-related changes in many species [16][17][18] , and considered the gold standard for small animal joints 19 . Unlike confined and unconfined compression tests, no sectioning or subsampling of tissue (i.e., cylindrical explants) is required 20 .…”

Section: Introductionmentioning

confidence: 99%

High spatial resolution analysis using indentation mapping differentiates biomechanical properties of normal vs. degenerated mouse articular cartilage

Masson

Besler

Edwards

et al. 2021

Preprint

View full text Add to dashboard Cite

Characterizing the biomechanical properties of articular cartilage is crucial to understanding processes of tissue homeostasis vs. degeneration. In mouse models, however, limitations are imposed by their small joint size and thin cartilage surfaces. Here we present a 3D automated surface mapping system and methodology that allows for mechanical characterization of mouse cartilage with high spatial resolution. We performed repeated indentation mappings, followed by cartilage thickness measurement via needle probing, at 31 predefined positions distributed over the medial and lateral femoral condyles of healthy mice. High-resolution 3D x-ray microscopy (XRM) imaging was used to validate tissue thickness measurements. The automated indentation mapping was reproducible, and needle probing yielded cartilage thicknesses comparable to XRM imaging. When comparing healthy vs. degenerated cartilage, topographical variations in biomechanics were identified, with altered thickness and stiffness (instantaneous modulus) across condyles and within anteroposterior sub-regions. This quantitative technique comprehensively characterized cartilage function in mice femoral condyle cartilage. Hence, it has the potential to improve our understanding of tissue structure-function interplay in mouse models of repair and disease.

show abstract

Mechanical, biochemical, and morphological topography of ovine knee cartilage

Cited by 10 publications

References 48 publications

Biomechanical, Morphological, and Biochemical Characteristics of Articular Cartilage of the Ovine Humeral Head

Biomechanical, Morphological, and Biochemical Characteristics of Articular Cartilage of the Ovine Humeral Head

Raman Needle Arthroscopy for In Vivo Molecular Assessment of Cartilage

High spatial resolution analysis using indentation mapping differentiates biomechanical properties of normal vs. degenerated mouse articular cartilage

Contact Info

Product

Resources

About