Mechanical Characterization for Cellular Mechanobiology: Current Trends and Future Prospects

Narasimhan, Badri Narayanan; Ting, Matthew S.; Kollmetz, Tarek; Horrocks, Matthew S; Chalard, Anaïs; Malmström, Jenny

doi:10.3389/fbioe.2020.595978

Cited by 34 publications

(26 citation statements)

References 125 publications

(111 reference statements)

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“…These can reveal mechanical properties across a wide spatial scale, from isolated cells, through muscle strands, sections of myocardium, to the whole heart. Recent work highlights developments for the assessment of mechanical properties at the cell level (Narasimhan et al 2020) even at high throughput (Romanov et al 2021).…”

Section: In Vitro Techniquesmentioning

confidence: 99%

Passive myocardial mechanical properties: meaning, measurement, models

et al. 2021

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Passive mechanical tissue properties are major determinants of myocardial contraction and relaxation and, thus, shape cardiac function. Tightly regulated, dynamically adapting throughout life, and affecting a host of cellular functions, passive tissue mechanics also contribute to cardiac dysfunction. Development of treatments and early identification of diseases requires better spatio-temporal characterisation of tissue mechanical properties and their underlying mechanisms. With this understanding, key regulators may be identified, providing pathways with potential to control and limit pathological development. Methodologies and models used to assess and mimic tissue mechanical properties are diverse, and available data are in part mutually contradictory. In this review, we define important concepts useful for characterising passive mechanical tissue properties, and compare a variety of in vitro and in vivo techniques that allow one to assess tissue mechanics. We give definitions of key terms, and summarise insight into determinants of myocardial stiffness in situ. We then provide an overview of common experimental models utilised to assess the role of environmental stiffness and composition, and its effects on cardiac cell and tissue function. Finally, promising future directions are outlined.

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Section: In Vitro Techniquesmentioning

confidence: 99%

Passive myocardial mechanical properties: meaning, measurement, models

et al. 2021

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“…[ 134 ] A detailed discussion on mechanical properties measured using different techniques and factors to consider in such measurements is reviewed elsewhere. [ 135,136 ]…”

Section: Physicochemical Considerationsmentioning

confidence: 99%

“…[134] A detailed discussion on mechanical properties measured using different techniques and factors to consider in such measurements is reviewed elsewhere. [135,136] For cell attachment to hydrogels, the gels should either contain adhesion domains or need to be covalently or noncovalently functionalized with adhesion proteins. For synthetic gels containing amide group such as PAAm, adhesion proteins are usually presented using Sulfo-SANPAH chemistry.…”

Section: Physicochemical Considerationsmentioning

confidence: 99%

Hydrogels with Tunable Physical Cues and Their Emerging Roles in Studies of Cellular Mechanotransduction

Narasimhan

Horrocks

Malmström

2021

Advanced NanoBiomed Research

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The extracellular matrix provides complex biophysical cues to cells which respond to these signals with signaling cascades that determine various cellular processes including fate. Many material systems have been explored to mimic the mechanical properties of the extracellular matrix to determine the cell responses to mechanical cues. While stiffness has emerged as an important regulator of cell behavior, recently, other mechanical properties such as strain stiffening and viscoelasticity have also emerged as potent regulators. This review explores the substrates used for studying mechanotransduction and strategies adopted to impart more complex mechanical cues including spatiotemporal control of mechanical properties. In addition, practical considerations for designing hydrogels for cell culture are discussed and the response of cells to viscoelastic cues in particular is discussed in depth. Recent mechanotransduction studies of combinations of mechanical and other cues are finally reviewed. It is anticipated that such multiphysical cues will further the understanding of mechanotransduction involved in complex processes such as migration and mechanical memory and provide a framework in controlling cell behavior.

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“…Mechanobiology describes how cell mechanics and mechanical forces influence cell behavior, cell morphogenesis, and diseases such as cancer [23,73]. In the context of mechanobiology, the mechanical properties of cells play significant roles in sensing and responding to their external surrounding [25,[73][74][75]. With the help of the cytoskeleton, cells can resist the deformation induced by their microenvironment and alter their shapes during movement by polymerizing or fluidizing the polymeric structure of the cytoskeleton [76][77][78].…”

Section: Nano-bio-interactions: Cell Mechanics and Mechanobiologymentioning

confidence: 99%

Cancer-Nano-Interaction: From Cellular Uptake to Mechanobiological Responses

Kashani

Packirisamy

2021

IJMS

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With the advancement of nanotechnology, the nano-bio-interaction field has emerged. It is essential to enhance our understanding of nano-bio-interaction in different aspects to design nanomedicines and improve their efficacy for therapeutic and diagnostic applications. Many researchers have extensively studied the toxicological responses of cancer cells to nano-bio-interaction, while their mechanobiological responses have been less investigated. The mechanobiological properties of cells such as elasticity and adhesion play vital roles in cellular functions and cancer progression. Many studies have noticed the impacts of cellular uptake on the structural organization of cells and, in return, the mechanobiology of human cells. Mechanobiological changes induced by the interactions of nanomaterials and cells could alter cellular functions and influence cancer progression. Hence, in addition to biological responses, the possible mechanobiological responses of treated cells should be monitored as a standard methodology to evaluate the efficiency of nanomedicines. Studying the cancer-nano-interaction in the context of cell mechanics takes our knowledge one step closer to designing safe and intelligent nanomedicines. In this review, we briefly discuss how the characteristic properties of nanoparticles influence cellular uptake. Then, we provide insight into the mechanobiological responses that may occur during the nano-bio-interactions, and finally, the important measurement techniques for the mechanobiological characterizations of cells are summarized and compared. Understanding the unknown mechanobiological responses to nano-bio-interaction will help with developing the application of nanoparticles to modulate cell mechanics for controlling cancer progression.

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Mechanical Characterization for Cellular Mechanobiology: Current Trends and Future Prospects

Cited by 34 publications

References 125 publications

Passive myocardial mechanical properties: meaning, measurement, models

Passive myocardial mechanical properties: meaning, measurement, models

Hydrogels with Tunable Physical Cues and Their Emerging Roles in Studies of Cellular Mechanotransduction

Cancer-Nano-Interaction: From Cellular Uptake to Mechanobiological Responses

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