Introduction: The development of hair care cosmetic formulations with ingredients obtained from natural sources is a challenge since it can compromise the stability and performance of formulations. The search for innovative ingredients obtained from natural sources is important to overcome this challenge. In addition, the evaluation of the physicomechanical and sensorial proprieties, stability and effectiveness of hair formulations are fundamental to obtain formulations with good acceptance by consumers. In this context, the aim of this study was to develop and evaluate the stability, physical-mechanical, and sensorial properties, as well as the efficacy of hair care formulations based on natural ingredients. Methods: Shampoo and conditioner formulations containing natural ingredients such as hydrolyzed rice peptides and polysaccharides obtained from plants and biotechnological processes were developed. Stability, spreadability, hardness, texture profile, rheological behaviour and sensory properties of these formulations were evaluated. In addition, the efficacy of hair care formulations was assessed by break force, combability and brightness of hair samples. Results: Shampoo formulation containing hydrolyzed rice peptides had better results in the texture, hardness and spreadability parameters. Conditioner formulations with polysaccharides showed better performance in the tests. Regarding the hair effectiveness tests, all treatments increased the mechanical strength of the hair fiber as well as the strength in the combing test, but only shampoo formulation containing hydrolysed rice peptides increased the brightness of the hair. Moreover, the sensory analysis showed a correlation with the efficacy test by instrumental measurements. Conclusion: This study has a significant contribution since it showed that it is possible to develop stable and efficacy formulations with ingredients obtained from a natural source and the importance of the correlation between instrumental measurement data and sensory analysis.