BACKGROUND: Regenerative powertrain systems can extend the operating durations of robotic lower-limb prostheses and exoskeletons. These energy-efficient biomechatronic devices have been exclusively designed and evaluated for level-ground walking. Building on previous investigations, this research assessed the lower-limb joint biomechanical powers during stand-tosit movements using inverse dynamics modelling to estimate the biomechanical energy available for electrical regeneration. METHODS: Nine participants performed 20 sitting and standing movements while lower-limb kinematics and ground reaction forces were measured. Following the biomechanical model design, dynamic parameter identification computed the subject-specific body segment inertial parameters that minimized the differences in ground reaction forces and moments between the experimental measurements and inverse dynamic simulations. Joint biomechanical powers were calculated from net joint torques and rotational velocities and numerically integrated over time to determine biomechanical energy. RESULTS: The hip joint generated the largest maximum negative biomechanical power (1.8 ± 0.5 W/kg), followed by the knee joint (0.8 ± 0.3 W/kg) and ankle joint (0.2 ± 0.1 W/kg). Negative biomechanical energy from the hip, knee, and ankle joints per stand-to-sit movement were 0.36 ± 0.06 J/kg, 0.16 ± 0.08 J/kg, and 0.03 ± 0.01 J/kg, respectively. CONCLUSIONS: Using previously published maximum energy regeneration efficiencies (i.e., approximately 37 %), robotic knee prostheses and exoskeletons could theoretically regenerate 0.06 ± 0.03 J/kg of electrical energy while sitting down. These findings have implications on design optimization of regenerative powertrains for energy-efficient lower-limb biomechatronic devices.