Mechanical induction of lateral root initiation in
            <i>Arabidopsis thaliana</i>

Ditengou, Franck Anicet; Teale, William; Kochersperger, Philip; Flittner, Karl Andreas; Kneuper, Irina; Graaff, Eric van der; Nziengui, Hugues; Pinosa, Francesco; Li, Xugang; Nitschke, Roland; Laux, Thomas; Palme, Klaus

doi:10.1073/pnas.0807814105

Cited by 263 publications

(258 citation statements)

References 32 publications

Supporting

Mentioning

240

Contrasting

Unclassified

Order By: Relevance

“…Molecular studies showed that auxin and its polar transport are the central regulators of lateral root formation. It has been reported that auxin-dependent nuclear signaling is necessary for lateral root formation (Ditengou et al, 2008). Optimum rooting response using IBA and NAA has been reported for several other medicinal plants including Syzygium francissi (Shatnawi et al, 2004), pear (Shatnawi et al, 2007), Achillea millefolium (Shatnawi, 2013), and Stevia rebaudiana (Zayova et al, 2013).…”

Section: In Vitro Root Formation and Acclimatizationmentioning

confidence: 99%

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

Sharmin¹,

Alam²,

Sheikh³

et al. 2013

Turk J Biol

View full text Add to dashboard Cite

IntroductionCurcuma aromatica Salisb. (Zingiberaceae), indigenous to South Asia, is a robust zingiber with stout underground rhizomes. This wild, aromatic, and attractive turmeric is probably the most useful among the turmeric members for its unique medicinal values. C. aromatica rhizome is a rich source of volatile oil, which consists of several major anti-tumor ingredients including demethoxycurcumin, β-elemene, curcumol, curdione, etc. (Zhou et al., 1997;Dulak, 2005). C. aromatica could promote blood circulation to remove blood stasis and treat cancers and angiogenesis (Kim et al., 2002). The oil infused via the hepatic artery has proven to exert ideal therapeutic effects in humans with primary liver cancer and rats with transplanted hepatoma (Cheng et al., 1999). Curcumin, the most prevalent active secondary ingredient in C. aromatica, acts as a promising agent in the treatment and/or prevention of Alzheimer's disease (Ringman et al., 2005). In addition, the rhizome is Abstract: A rapid and improved micropropagation protocol was developed for Curcuma aromatica, a threatened aromatic medicinal plant, using rhizome sprout as the explant. Stepwise optimization of different plant growth regulators, carbon sources, and basal media was adopted to establish an efficient micropropagation protocol. When cytokinins, such as benzyl amino purine (BAP) or 6-(α,αdimethylallylamino)-purine (2iP), were used either singly or in combination with naphthalene acetic acid (NAA) for shoot induction and multiplication, a single use of BAP was the most effective. As a carbon source, 3% (w/v) sucrose exhibits the greatest promotive effect on shoot initiation and proliferation compared with other carbon sources used. Among the basal media, full strength Murashige and Skoog (MS) media produced the best results, compared to other media studied. By using the most effective treatment from each category, an average of 13.2 shoots/per explant were produced after 6 weeks of culture. Moreover, 85% survival was achieved when rooted explants acclimatized ex vitro using a mixture of sterile sand, soil, and farmyard manure (1:1:1). In addition, antimicrobial activities of rhizome extracts were evaluated. Petroleum ether and chloroform extracts of field-grown rhizome showed potential antimicrobial properties against several human pathogenic bacteria including Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella sonnei, and Shigella dysenteriae, with a minimum inhibitory concentration (MIC) ranging from 0.03 to 0.5 mg/mL. Thus, the optimized micropropagation protocol may offer large-scale production of plantlets to meet industrial demand for the rhizome. Moreover, our results suggest the rhizome extract of C. aromatica is a promising antimicrobial agent.

show abstract

Section: In Vitro Root Formation and Acclimatizationmentioning

confidence: 99%

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

Sharmin¹,

Alam²,

Sheikh³

et al. 2013

Turk J Biol

View full text Add to dashboard Cite

show abstract

“…The seedlings were then incubated in 1 mM 5-bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, 100 mM potasium phosphate buffer (pH 7.0), 1 mM EDTA, 1 mM potassium hexacyanoferrate, 1 mM potassium hexacyanoferrate, and 0.3% Triton X-100 overnight at 37°C. For visualization of blue staining the chlorophyll was removed by incubation in 70% ethanol (34).…”

Section: Methodsmentioning

confidence: 99%

Thioredoxin-insensitive plastid ATP synthase that performs moonlighting functions

Kohzuma

Bosco

Kanazawa

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The chloroplast ATP synthase catalyzes the light-driven synthesis of ATP and acts as a key feedback regulatory component of photosynthesis. Arabidopsis possesses two homologues of the regulatory γ subunit of the ATP synthase, encoded by the ATPC1 and ATPC2 genes. Using a series of mutants, we show that both these subunits can support photosynthetic ATP synthesis in vivo with similar specific activities, but that in wild-type plants, only γ 1 is involved in ATP synthesis in photosynthesis. The γ 1 -containing ATP synthase shows classical light-induced redox regulation, whereas the mutant expressing only γ 2 -ATP synthase (gamma exchangerevised ATP synthase, gamera) shows equally high ATP synthase activity in the light and dark. In situ redox titrations demonstrate that the regulatory thiol groups on γ 2 -ATP synthase remain reduced under physiological conditions but can be oxidized by the strong oxidant diamide, implying that the redox potential for the thiol/ disulphide transition in γ 2 is substantially higher than that for γ 1 . This regulatory difference may be attributed to alterations in the residues near the redox-active thiols. We propose that γ 2 -ATP synthase functions to catalyze ATP hydrolysis-driven proton translocation in nonphotosynthetic plastids, maintaining a sufficient transthylakoid proton gradient to drive protein translocation or other processes. Consistent with this interpretation, ATPC2 is predominantly expressed in the root, whereas modifying its expression results in alteration of root hair development. Phylogenetic analysis suggests that γ 2 originated from ancient gene duplication, resulting in divergent evolution of functionally distinct ATP synthase complexes in dicots and mosses. plant development | bioenergetics L ight-driven linear electron flow (LEF) in photosynthesis stores energy in reduced NADPH. It is also coupled to the translocation of protons into the thylakoid lumen, generating an electrochemical gradient of protons, the proton motive force (pmf), across the thylakoid. The pmf in turn drives the synthesis of ATP from ATP and P i via the CF O -CF 1 ATP synthase (ATP synthase), storing energy in the form of the photophosphorylation potential. The plastid ATP synthase complex consists of nine different sub-

show abstract

“…Immunolocalization of PIN1 was performed using an affinity-purified rabbit anti-PIN1 antibody as described by Gälweiler et al (1998). Dilutions of primary anti-PIN2 guinea pig antibody (Ditengou et al, 2008) was 1:600 and for the anti-PM H + -ATPase rabbit antibody (Langhans et al, 2001; Agrisera AS07 260) 1:1000. As secondary antibodies, ALEXA Fluor 488 goat anti-guinea pig (Invitrogen) and ALEXA Fluor 555 goat anti-rabbit (Invitrogen) diluted to 1:600 were used.…”

Section: Histochemical Gus and Starch Staining And Immunolocalizationmentioning

confidence: 99%

Inactivation of Plasma Membrane–Localized CDPK-RELATED KINASE5 Decelerates PIN2 Exocytosis and Root Gravitropic Response inArabidopsis

et al. 2013

Self Cite

View full text Add to dashboard Cite

CRK5 is a member of the Arabidopsis thaliana Ca 2+ /calmodulin-dependent kinase-related kinase family. Here, we show that inactivation of CRK5 inhibits primary root elongation and delays gravitropic bending of shoots and roots. Reduced activity of the auxin-induced DR5-green fluorescent protein reporter suggests that auxin is depleted from crk5 root tips. However, no tip collapse is observed and the transcription of genes for auxin biosynthesis, AUXIN TRANSPORTER/AUXIN TRANSPORTER-LIKE PROTEIN (AUX/LAX) auxin influx, and PIN-FORMED (PIN) efflux carriers is unaffected by the crk5 mutation. Whereas AUX1, PIN1, PIN3, PIN4, and PIN7 display normal localization, PIN2 is depleted from apical membranes of epidermal cells and shows basal to apical relocalization in the cortex of the crk5 root transition zone. This, together with an increase in the number of crk5 lateral root primordia, suggests facilitated auxin efflux through the cortex toward the elongation zone. CRK5 is a plasma membrane-associated kinase that forms U-shaped patterns facing outer lateral walls of epidermis and cortex cells. Brefeldin inhibition of exocytosis stimulates CRK5 internalization into brefeldin bodies. CRK5 phosphorylates the hydrophilic loop of PIN2 in vitro, and PIN2 shows accelerated accumulation in brefeldin bodies in the crk5 mutant. Delayed gravitropic response of the crk5 mutant thus likely reflects defective phosphorylation of PIN2 and deceleration of its brefeldin-sensitive membrane recycling.

show abstract

Mechanical induction of lateral root initiation in Arabidopsis thaliana

Cited by 263 publications

References 32 publications

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant

Thioredoxin-insensitive plastid ATP synthase that performs moonlighting functions

Inactivation of Plasma Membrane–Localized CDPK-RELATED KINASE5 Decelerates PIN2 Exocytosis and Root Gravitropic Response inArabidopsis

Contact Info

Product

Resources

About