Mechanical model of Golgi apparatus

Jelerčič, Urška

doi:10.1088/1478-3975/ab3766

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…Light & electron microscopy [52][53][54][55][56] , proteomics [57][58][59] and mechanistic models 37,46,[60][61][62] have all contributed in crucial ways to the current knowledge base of Golgi architecture. One common drawback, however, of all these approaches is that they all provide single readouts (Golgi phenotype, interaction pairs, single gene expression).…”

Section: Introductionmentioning

confidence: 99%

Phenotype-based single-cell transcriptomics reveal compensatory pathways involved in Golgi organization and associated transport

Singh

Zukowska

Halavatyi

et al. 2022

Preprint

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The Golgi is a dynamic organelle with a unique morphology that has implications on its function. How the structural integrity of the Golgi is maintained despite its dynamic nature has been a long-standing question. Several siRNA-based screens have addressed this question and have identified a number of key players required for Golgi integrity. Interestingly, they also reported heterogeneity of phenotypic responses with regards to Golgi morphology. Although never systematically investigated, this variability has generally been attributed to poor transfection efficiency or cell cycle specific responses. Here we show that this heterogeneity is the result of differential response to the siRNA knockdown in different Golgi phenotypes, independent of transfection efficiency or cell cycle phases. To characterize the observed Golgi phenotype-specific responses at the molecular level we have developed an automated assay which enables microscopy-based phenotype classification followed by phenotype-specific single-cell transcriptome analysis. Application of this novel approach to the siRNA mediated knockdown of USO1, a key trafficking protein at the ER to Golgi boundary, surprisingly suggests a key involvement of the late endosomal/endocytic pathways in the regulation of Golgi organization. Our pipeline is the first of its kind developed to study Golgi organization, but can be applied to any biological problem that stands to gain from correlating morphology with single-cell readouts. Moreover, its automated and modular nature allows for uncomplicated scaling up, both in throughput and in complexity, helping the user achieve a systems level understanding of cellular processes.

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Section: Introductionmentioning

confidence: 99%

Phenotype-based single-cell transcriptomics reveal compensatory pathways involved in Golgi organization and associated transport

Singh

Zukowska

Halavatyi

et al. 2022

Preprint

View full text Add to dashboard Cite

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Involvement of Intestinal Goblet Cells and Changes in Sodium Glucose Transporters Expression: Possible Therapeutic Targets in Autistic BTBR T+Itpr3tf/J Mice

Franco

Bonomini

Borsani

et al. 2021

IJERPH

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Autism spectrum disorder is a neurodevelopmental syndrome with a complicated etiology and could be responsible for disrupted gastrointestinal tract microbiota. The aim of this work was to study intestinal samples from an autistic animal model (BTBR mouse strain) to better describe gastrointestinal alterations. We performed a morphological and biological evaluation of small intestine samples. In terms of morphology, we studied the goblet cells, cells of intestinal mucosal responsible for the production and maintenance of the protective mucous blanket. Alterations in their secretion may indicate an altered rate of mucus synthesis and this is one of the possible causes of gastrointestinal problems. In terms of biological evaluation, impaired regulation of glucose homeostasis regulated by sodium-glucose transporters has been suggested as an important component of obesity and associated comorbidities; therefore, this study analyzed the expression of sodium/glucose transporter-1 and -3 in BTBR mice to better define their role. We demonstrated that, in BTBR mice as compared to C57BL/6J (B6) strain animals: (1) The goblet cells had different protein content in their vesicles and apparently a larger number of Golgi cisternae; (2) the expression and level of sodium/glucose transporters were higher. These findings could suggest new possible targets in autism spectrum disorder to maintain mucus barrier function.

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Mechanical model of Golgi apparatus

Cited by 2 publications

References 40 publications

Phenotype-based single-cell transcriptomics reveal compensatory pathways involved in Golgi organization and associated transport

Phenotype-based single-cell transcriptomics reveal compensatory pathways involved in Golgi organization and associated transport

Involvement of Intestinal Goblet Cells and Changes in Sodium Glucose Transporters Expression: Possible Therapeutic Targets in Autistic BTBR T+Itpr3tf/J Mice

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