Mechanical phenotyping of K562 cells by the Micropipette Aspiration Technique allows identifying mechanical changes induced by drugs

Cerbo, Alessandro Di; Rubino, Valentina; Morelli, Francesca; Ruggiero, Giuseppina; Landi, Rosaria; Guidetti, Gianandrea; Canello, Sergio; Terrazzano, Giuseppe; Alessandrini, Andrea

doi:10.1038/s41598-018-19563-z

Cited by 30 publications

(22 citation statements)

References 64 publications

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“…[131], disease prevention, morbidity and mortality reduction and growth promotion [87,88] DNA synthesis reduction [112], polymorphonuclear leukocyte oxygen consumption reduction [114], environmental, manure, soil, surface waters, groundwater and wastewater pollution [119, 123 -125] Achromycin malaria [19,132] Oxytetracycline Entamoeba histolytica infection (Amebiasis) [133,134], Trichomonas vaginalis infection (Trichomoniasis) and Leishmania major infection (Leishmaniasis) [135], Giardia lamblia infection (Giardiasis) [133,135], food intolerances induction [115] Giardia lamblia infection (Giardiasis) [133], disease prevention, morbidity and mortality reduction and growth promotion [87,88,127], adverse food reactions induction [15,101] pro-apoptotic effect [100,106], proinflammatory effect [105,107,109] [35 -37, 40 -42], suppurative hidradenitis [52], chronic pityriasis lichenoides [56], metastatic prostate cancer [66] filarial nematodes [21], Giardia lamblia infection (Giardiasis) [133], Leishmania major infection (Leishmaniasis) [131], digital dermatitis [93] environmental, manure, soil, and surface waters, groundwater and wastewater pollution [120, 123-124-125] Thiatetracycline [58 -60], scleroderma [61,62], acute ischemic stroke [76], fragile X syndrome [82], acute encephalitis syndrome [83], schizophrenia [86] filarial nematodes [137] groundwater and surface water pollution [124] COL-3…”

Section: Resultsmentioning

confidence: 99%

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Tetracyclines: Insights and Updates of their Use in Human and Animal Pathology and their Potential Toxicity

Cerbo¹,

Pezzuto²,

Guidetti³

et al. 2019

Open Biochem J.

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Tetracycline antibiotics (TCs) have been widely employed to treat bacterial infections and other pathologic conditions in humans and pets. Although most of TCs have been almost ruled out from the human clinical practice they are still used as growth promoters and to treat promiscuity and overcrowding pathologies in the intensive animal farming. As a consequence, TCs are commonly found in all ecological compartments with potential direct or indirect toxicological effects on animals and, generally, on all living organisms. Moreover, clinical and in vitro observations raised the hypothesis that the widespread of some adverse food reactions and, to a less extent, antibiotic resistance phenomena could be ascribed to the presence of TCs residues in edible and non-edible tissues of intensive animal farming intended for animal and human consumption. Such residues may pose serious health threat, depending on the type of food and the amount of residue present.The aim of this review is to provide new insights about the clinical uses of TCs in humans and animals and their potential toxic effects as residues in the environment or as food components.

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Section: Resultsmentioning

confidence: 99%

“…However, further experiments on K562 cells challenged with OTC for different incubation times, showed apoptotic features (i.e. blebs formation) already after 12h of incubation by means of Micropipette Aspiration technique [106].…”

Section: Potential Toxic Effects Of Tcsmentioning

confidence: 93%

Tetracyclines: Insights and Updates of their Use in Human and Animal Pathology and their Potential Toxicity

Cerbo¹,

Pezzuto²,

Guidetti³

et al. 2019

Open Biochem J.

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“…5). Although there is evidence that cell mechanical properties are linked with immune cell functions (16,17,51), mechanistic knowledge of how physiologic cues elicit such physical changes is still developing. Our results discriminate between the roles of formin-nucleated and Arp2/ 3-nucleated actin filaments in stress hormone regulation of macrophage deformability: our findings suggest that b-AR activation shifts the balance between actin filament populations to favor more highly branched, Arp2/ 3-mediated structures rather than linear unbranched filaments ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Stress hormone signaling through β‐adrenergic receptors regulates macrophage mechanotype and function

Kim

Christodoulides

et al. 2018

FASEB j.

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Critical functions of immune cells require them to rapidly change their shape and generate forces in response to cues from their surrounding environment. However, little is known about how soluble factors that may be present in the microenvironment modulate key aspects of cellular mechanobiology—such as immune cell deformability and force generation—to impact functions such as phagocytosis and migration. Here we show that signaling by soluble stress hormones through β‐adrenoceptors (β‐AR) reduces the deformability of macrophages; this is dependent on changes in the organization of the actin cytoskeleton and is associated with functional changes in phagocytosis and migration. Pharmacologic interventions reveal that the impact of β‐AR signaling on macrophage deformability is dependent on actin‐related proteins 2/3, indicating that stress hormone signaling through β‐AR shifts actin organization to favor branched structures rather than linear unbranched actin filaments. These findings show that through remodeling of the actin cytoskeleton, β‐AR‐mediated stress hormone signaling modulates macrophage mechanotype to impact functions that play a critical role in immune response.—Kim, T.‐H., Ly, C., Christodoulides, A., Nowell, C. J., Gunning, P. W., Sloan, E. K., Rowat, A. C. Stress hormone signaling through β‐adrenergic receptors regulates macrophage mechanotype and function. FASEB J. 33, 3997–4006 (2019). http://www.fasebj.org

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“…Furthermore, the deformability of the cells also affects their morphological and morphometrical properties (relating to cell diameter and height) as well as elastic properties (Young's modulus) [4,5]. These changes might be due to the altered cytoskeletal filament network (e.g., actin, microtubules, or lamins) organization of the cells [8,9]. By understanding these properties, essential information may be obtained for further cell rheology studies crucial to understanding disease and improving health.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic device for rapid investigation of the deformability of leukocytes in whole blood samples

2020

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The mechanical properties of cells, such as leukocytes, in a diseased state differ from those of healthy cells, typically due to their microstructure. The deformability of the cells through a constrictive area is analyzed by the applied stress to the cell. This study investigates the relationship between the sample flow speed and distribution of captured leukocytes based on the cell deformability using a microfluidic device. The device comprises of microfilters that serve as the filtration mechanism. The microfilter gap size gradually decreases from 15 to 3 µm to facilitate the deformabilitybased separation. Leukocytes have various sizes; hence, they can be separated by microfilters directly from whole blood samples without any cell clogging, and they do not require sample pre-processing such as centrifugation or red blood cell lysis. The distribution of leukocytes captured by the microfilters with respect to the sample flow speed can be analyzed; at higher sample flow speeds of 6 µL/min, small leukocytes with a size of 7 µm could not be captured and they passed through the smallest microfilter gap size of 3 µm. For smaller leukocytes, such as lymphocytes, the distributions are mainly at gap sizes of 4 µm to 8 µm, with most of the lymphocytes captured at the 6 µm microfilter gap size. We conclude that the distribution of the cells captured during the filtration varies depending on the microfilter gap sizes, applied sample flow speed, cell sizes, and the ability of the cells to deform. The deformability imaging profiles of the sample could be developed from the images of the cell distribution, which might be useful for preliminary screening in the clinical applications. This work presents the development of a simple device for the study of cell deformability as the results provide a biophysical marker in high throughput and bulk sample analyses.

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Mechanical phenotyping of K562 cells by the Micropipette Aspiration Technique allows identifying mechanical changes induced by drugs

Cited by 30 publications

References 64 publications

Tetracyclines: Insights and Updates of their Use in Human and Animal Pathology and their Potential Toxicity

Tetracyclines: Insights and Updates of their Use in Human and Animal Pathology and their Potential Toxicity

Stress hormone signaling through β‐adrenergic receptors regulates macrophage mechanotype and function

Microfluidic device for rapid investigation of the deformability of leukocytes in whole blood samples

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