Mechanical properties and failure analysis of visible light crosslinked alginate-based tissue sealants

Charron, Patrick N.; Fenn, Spencer L.; Poniz, Alex; Oldinski, Rachael A.

doi:10.1016/j.jmbbm.2016.02.003

Cited by 35 publications

(72 citation statements)

References 30 publications

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“…A 1% (w/v) polymer solution in D 2 O was analyzed at room temperature, spinning at 20 Hz for 16 scans. 28, 30 The DOM was quantified by peak integration and calculation of the ratio between of the methyl protons at 2.0 ppm and the newly formed methylene protons of methacrylate at 5.75 ppm and 6.25 ppm. 30-31 …”

Section: Methodsmentioning

confidence: 99%

Dual-Cross-Linked Methacrylated Alginate Sub-Microspheres for Intracellular Chemotherapeutic Delivery

Fenn

Miao

Scherrer

et al. 2016

ACS Appl. Mater. Interfaces

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Intracellular delivery vehicles comprised of methacrylated alginate (Alg-MA) were developed for the internalization and release of doxorubicin hydrochloride (DOX). Alg-MA was synthesized via an anhydrous reaction, and a mixture of Alg-MA and DOX was formed into sub-microspheres using a water/oil emulsion. Covalently crosslinked sub-microspheres were formed via exposure to green light, in order to investigate effects of crosslinking on drug release and cell internalization, compared to traditional techniques such as ultra violet (UV) light. Crosslinking was performed using light exposure alone, or in combination with ionic crosslinking using calcium chloride (CaCl2). Alg-MA sub-microsphere diameters were between 88 – 617 nm, and zeta-potentials were between −20 and −37 mV. Using human lung epithelial carcinoma cells (A549s) as a model, cellular internalization was confirmed using flow cytometry; different sub-microsphere formulations varied the efficiency of internalization, with UV-crosslinked sub-microspheres achieving the highest internalization percentages. While blank (non-loaded) Alg-MA sub-microspheres were non-cytotoxic to A549s, DOX-loaded sub-microspheres significantly reduced mitochondrial activity after five days of culture. Photo-crosslinked Alg-MA sub-microspheres may be a potential chemotherapeutic delivery system for cancer treatment.

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Section: Methodsmentioning

confidence: 99%

Dual-Cross-Linked Methacrylated Alginate Sub-Microspheres for Intracellular Chemotherapeutic Delivery

Fenn

Miao

Scherrer

et al. 2016

ACS Appl. Mater. Interfaces

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“…24, 56 Briefly, a 2% (w/v) solution of Manugel® GMB (MW≈ 170–240 kDa, FMC Biopolymer) in deionized (DI) water was mixed with a 20-fold molar excess of methacrylic anhydride (Sigma-Aldrich). The solution was maintained at pH 8 using 5 N sodium hydroxide (Fisher) for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

“…56, 62 Briefly, a polyether ether ketone device was designed to securely fasten a membrane/substrate across an open chamber, using a fluoroelastomer o-ring and clamp, which can be pressurized using an air-filled syringe and syringe pump (PHD 2000 Infuser, Harvard Apparatus). Collagen-rich substrates (Collagen Casings, The Sausage Maker Inc.) were used as an in vitro test membrane per the standard.…”

Section: Methodsmentioning

confidence: 99%

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Anticancer Therapeutic Alginate-Based Tissue Sealants for Lung Repair

Fenn

Charron

Oldinski

2017

ACS Appl. Mater. Interfaces

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Injury to the connective tissue that lines the lung, the pleura, or to the lung itself can occur from many causes including trauma or surgery, as well as lung diseases or cancers. To address current limitations for patching lung injuries, to stop air or fluid leaks, an adherent hydrogel sealant patch system was developed, based on methacrylated alginate (AMA) and AMA di-aldehyde (AMA-DA) blends, which is capable of sealing damaged tissues and sustaining physiological pressures. Methacrylation of alginate hydroxyl groups rendered the polysaccharide capable of photo-crosslinking when mixed with an eosin Y-based photo-initiator system, and exposed to visible green light. Oxidation of alginate yields functional aldehyde groups capable of imine bond formation with proteins found in many tissues. The alginate-based patch system was rigorously tested on a custom burst pressure testing device. Blending of non-oxidized material with oxidized (aldehyde modified) alginates yielded patches with improved burst pressure performance, and decreased delamination as compared with pure AMA. Human mesothelial cell (MeT-5A) viability and cytotoxicity were retained when cultured with the hydrogel patches. The release and bioactivity of doxorubicin-encapsulated sub-microspheres enabled the fabrication of drug-eluting adhesive patches, and were effective in decreasing human lung cancer cell (A549) viability.

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“…Common photocurable polymers employ UV radiation for curing, which often results in the denaturation of biomolecules and a subsequent loss of bioactivity [168,169]. To overcome this drawback, Seo et al switched to the use of visible light by modifying chitosan with photocurable furfuryl moieties and visible light initiator systems [136,170,171].…”

Section: Medical Applicationsmentioning

confidence: 99%