Mechanical properties of arteries

Dobrin, Philip B.

doi:10.1152/physrev.1978.58.2.397

Cited by 673 publications

(452 citation statements)

References 55 publications

(68 reference statements)

Supporting

Mentioning

407

Contrasting

Unclassified

Order By: Relevance

“…Breakdown or structural damage to capillary walls resulting from disease may provide one situation in which vascular cells experience altered loading (33); exercise may also provide this type of stimulus, and previous studies have noted alterations in capillary densities with exercise (34). Previously calculated strain levels in capillary walls during systolic contraction and stable diastolic arrest range between 0 to 22% (30,35), supporting the physiologic relevance of the 7% strain amplitude used in of our studies (29). Other investigations have used similar strain levels to study the effects of mechanical cue effects on ECs in vitro, specifically in the context of angiogenesis (36)(37)(38).…”

Section: Discussionsupporting

confidence: 83%

“…In vitro sprouting was used as a model for the initial stages of angiogenesis, as previously described (25)(26)(27)(28). Vascular cell cultures were exposed to cyclic tensile strain with an amplitude of 7%, representative of that experienced by vascular cells in large vessels, and also in the same range as previous calculations of capillary wall strain (29,30). Cyclic tensile strain was demonstrated to alter vascular cell phenotype and the secretion of Ang-2 and PDGF by EC.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Cyclic tensile strain triggers a sequence of autocrine and paracrine signaling to regulate angiogenic sprouting in human vascular cells

Yung

Chae

Buehler

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Mechanical signals regulate blood vessel development in vivo, and have been demonstrated to regulate signal transduction of endothelial cell (EC) and smooth muscle cell (SMC) phenotype in vitro. However, it is unclear how the complex process of angiogenesis, which involves multiple cell types and growth factors that act in a spatiotemporally regulated manner, is triggered by a mechanical input. Here, we describe a mechanism for modulating vascular cells during sequential stages of an in vitro model of early angiogenesis by applying cyclic tensile strain. Cyclic strain of human umbilical vein (HUV)ECs up-regulated the secretion of angiopoietin (Ang)-2 and PDGF-␤␤, and enhanced endothelial migration and sprout formation, whereas effects were eliminated with shRNA knockdown of endogenous Ang-2. Applying strain to colonies of HUVEC, cocultured on the same micropatterned substrate with nonstrained human aortic (HA)SMCs, led to a directed migration of the HASMC toward migrating HUVECs, with diminished recruitment when PDGF receptors were neutralized. These results demonstrate that a singular mechanical cue (cyclic tensile strain) can trigger a cascade of autocrine and paracrine signaling events between ECs and SMCs critical to the angiogenic process.angiogenesis ͉ angiopoietin-2 ͉ endothelial cells ͉ shRNA ͉ strain gradient

show abstract

Section: Discussionsupporting

confidence: 83%

mentioning

confidence: 99%

Cyclic tensile strain triggers a sequence of autocrine and paracrine signaling to regulate angiogenic sprouting in human vascular cells

Yung

Chae

Buehler

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…The common occurrence of respiratory insufficiency leads to elevated partial pressures of carbon dioxide, causing vasodilation. Glycogen-filled vacuoles in the wall of resistance vessels (Kretzschmar et al 1990) could disturb the production of extracellular matrix proteins like collagen and elastin (Dobrin 1978), of matrix metalloproteinases (Loftus and Thompson 2002), or of vasoactive substances like nitric oxide (McCarron et al 2006). We suppose that confounding cardiovascular risk factors leading to cerebral microangiopathy diminish the effect of dilated resistance vessels on RI.…”

Section: Discussionmentioning

confidence: 99%

Morphology and Function of Cerebral Arteries in Adults with Pompe Disease

et al. 2014

View full text Add to dashboard Cite

Objective: Cerebrovascular abnormalities have been reported in adult patients with Pompe disease. The objective was to study these abnormalities by (1) determining the diameter and mean flow velocity (MFV) of large cerebral arteries and (2) estimating cerebral blood flow (CBF), resistance index (RI) and cerebrovascular reactivity (CVR) as functions of resistance vessels.Methods: In ten adults with Pompe disease and twenty controls, the diameter, peak systolic (PSV) and enddiastolic velocities (EDV) of arteries supplying the brain were quantified by MR angiography and sonography. MFV, RI and CBF were calculated. CVR in the middle cerebral artery (MCA) was determined by hyperventilation and acetazolamide injection.Results: MR angiography revealed dilation of cerebral arteries predominantly in the posterior circulation. Dilative arteriopathy was found in three patients; two of them showed vertebrobasilar dolichoectasia. Despite of the dilative arteriopathy, the MFV was normal, indicating increased CBF and dilated resistance vessels. RI of all examined arteries and CVR of MCA were normal.Conclusion: The data suggest that dilation of small and large cerebral arteries is a common feature in adults with Pompe disease. Increased CBF might be the consequence of dilated resistance vessels. However, dysfunction of resistance vessels was rarely found.Synopsis: In adults with Pompe disease, dilation of small and large cerebral arteries is a common feature and might be associated with increased cerebral blood flow.

show abstract

“…Due to the noniinearity of the stress-strain curves, the incremental modulus (E^ was calculated according to the methods of McDonald 29 and Dobrin, 32 using stress increments (Aa) of 1.5 x 10 s Nm~2, according to the formula:…”

Section: Dynamometrymentioning

confidence: 99%

Age-related changes in composition and mechanical properties of the tunica media of the upper thoracic human aorta.

Spina¹,

Garbisa²,

Hinnie³

et al. 1983

Arteriosclerosis

102

View full text Add to dashboard Cite

A cylindrical segment, free of complex atherosclerotic lesions, was resected at autopsy from each of 59 descending human thoracic aortas by cutting just below the level of the first pair of intercostal arteries and 35 mm distal to this incision. Each isolated tunica media was defatted and subjected to successive treatment with EDTATris, 5 M guanidine hydrochloride-Tris, 5 M guanidine hydrochloride-Tris-DTE, collagenase and either trypsin or hot alkali. After each extraction or digestion, the dimensions and weight of the segments were measured and the extracted materials were analyzed and quantltated. This allowed the total content of the various components of the tunica media to be assessed by both gravimetric and analytical means. An agerelated rise was observed in the total content of the following components: proteins and glycoproteins soluble in chaotropic solvents (ranging from 24 mg/cm in the youngest samples to 46 mg/cm in the oldest) and collagen (38 mg/cm to 69 mg/cm).In contrast, the total content of elastin remained constant at 70 mg/cm at all ages, but its concentration decreased due to the rise in the concentration of the other tissue components as the tunica media thickened with age. It was also noted that with increasing age there was an accumulation of protein(s) which could not be solubilized by extraction with chaotropic agents or with collagenase, but which could be removed by treatment with either trypsin or hot alkali. Mechanical measurements conducted before and after trypsin digestion on samples previously subjected to purification with the first four agents used suggest that this accumulated protein(s) influenced the elastic response of the tissue to the applied stress by increasing the incremental modulus, the breaking stress, and the hysteresis. After the removal of this additional protein(s), the mechanical behavior of the elastin component was found to be Identical in all samples, irrespective of age. It is therefore proposed that the morphological changes and the stiffening observed in the aging aortic wall are not due to degradation of its elastin network but to variations in the supramolecular organization of connective tissue components. (Arteriosclerosis 3:64-76, January/February 1983) W ith advancing age, the human aorta dilates and becomes stiffer at physiological pressures. To date, investigations aimed at the identification of compositional or structural changes of the wall components responsible for these alterations have produced rather conflicting results. 6 " 9 This probably reflects differences in sampling or in the methodologies adopted for the isolation and quantitation of ma- trix components, complicated by the use of relative, rather than absolute, concentrations in the expression of the results. 10 In addition, it is not yet known whether the mechanical properties of the aortic collagen fibers change with aging, while data relating to the dynamics of the elastin component, which is responsible for the long-range reversible extensibility of the vessel, are largely u...

show abstract

Mechanical properties of arteries

Cited by 673 publications

References 55 publications

Cyclic tensile strain triggers a sequence of autocrine and paracrine signaling to regulate angiogenic sprouting in human vascular cells

Cyclic tensile strain triggers a sequence of autocrine and paracrine signaling to regulate angiogenic sprouting in human vascular cells

Morphology and Function of Cerebral Arteries in Adults with Pompe Disease

Age-related changes in composition and mechanical properties of the tunica media of the upper thoracic human aorta.

Contact Info

Product

Resources

About