Fruits and vegetable (F&V) account for a large proportion of the market output, and reducing mechanical damage can improve economic benefits. During harvesting and storage of F&V, visible mechanical damage and unseen bruises can result from external loads. However, there are relatively few studies on the micro‐damage of F&V, and the micro‐damage changes are reflected in the macro. Therefore, it is necessary to establish macroscopic, mesoscopic, and microscopic mechanical structure models. In this paper, the factors influencing the mechanics of F&V are analyzed, macroscopic, mesoscopic, and microscopic mechanical analysis methods of F&V are reviewed, multi‐scale mechanical relationships are explored, and future research directions of multi‐scale biomechanics of F&V are prospected. The results show that future research direction should mainly focus on the force analysis of microscopic single cell, as it is the basis of mechanical analysis of F&V.