Mechanical properties of films and three-dimensional scaffolds made of fibroin and gelatin

Bagrov, D. V.; Жуйков, В. А.; Chudinova, Yu. V.; Yarisheva, A.; Kotlyarova, M. S.; Arkhipova, A. Yu.; Khaydapova, D. D.; Moisenovich, Mikhail M.; Шайтан, К. В.

doi:10.1134/s0006350917010031

Cited by 6 publications

(8 citation statements)

References 42 publications

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“…There is a statistically significant difference on day 7 in the treated samples compared to the untreated ones, with a p value <0.05. Srivastava et al (2015) , Bagrov et al (2017) , and Bie et al (2015) reported absorption in samples treated with 15% MeOH and untreated between 36 and 46%, respectively. These values are similar to those obtained in this study.…”

Section: Resultsmentioning

confidence: 99%

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Effects of Chemical Post-treatments on Structural and Physicochemical Properties of Silk Fibroin Films Obtained From Silk Fibrous Waste

Puerta

Peresin

Restrepo‐Osorio

2020

Front. Bioeng. Biotechnol.

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Silk fibroin (SF) is a protein polymer claimed to have outstanding potential for medical applications. However, because of the manufacturing process, materials from regenerated SF exhibit a higher percentage of amorphous structures. The amorphous structures cause the material to be water soluble and can significantly limit its applications in wet biological environments. In order to increase the amount of crystalline structures and decrease the water solubility of SF materials, post-treatment with alcohols is usually employed. SF can be obtained from silk fibrous wastes (SFW), usually discarded in silk textile processes. This represents an opportunity to produce materials with high added value from low-cost natural sources. In this study, SF was obtained from SFW, and films were made thereof followed by a post-treatment by immersion or in a saturated atmosphere of methanol (MeOH) or ethanol (EtOH), using different exposure times. The resulting films were analyzed according to crystallinity, the percentage of crystalline and amorphous structures, and thermal stability. Also, water absorption and weight loss in aqueous media were determined. The results showed a significant increase in crystalline structures in all treated samples, varying according to the type and time of exposure to post-treatment conducted. The highest increase was shown in the case of the post-treatment by immersion in MeOH for 1 h, with a 23% increase over the untreated sample. This increase in crystallinity was reflected in an increase in the degradation temperature and a degradation rate of 5.3% on day 7. The possibility of tuning the degree of crystallinity, as well as thermal stability and aqueous integrity of thin films of SFW, can be applied to adjust these materials to the requirements of specific biomedical applications.

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Section: Resultsmentioning

confidence: 99%

“…To determine the effect of post-treatment time, the properties were evaluated after 15 min and 1 h of immersion/vapor exposure. These times were chosen according to previous reports ( Mandal et al, 2009 ; Bie et al, 2015 ; Srivastava et al, 2015 ; Zhou et al, 2016 ; Bagrov et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of Chemical Post-treatments on Structural and Physicochemical Properties of Silk Fibroin Films Obtained From Silk Fibrous Waste

Puerta

Peresin

Restrepo‐Osorio

2020

Front. Bioeng. Biotechnol.

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“…Формирование скаффолдов. СПГ и СП получали из водных растворов фиброина согласно методике, описанной ранее [8].…”

Section: материалы и методыunclassified

Bioresorbable Scaffolds Based on Fibroin for Bone Tissue Regeneration

Котлярова

Arkhipova

Moysenovich

et al. 2017

Moscow Univ. Biol.Sci. Bull.

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“…14 Singh et al have produced 3D printable bio ink by blending B. mori silk fibroin and gelatin for cartilage tissue engineering. 15 The mechanical properties of B. mori silk fibroin/gelatin scaffolds have been studied by Bagrov et al 16 A few researchers have electrospun B. mori silk fibroin/gelatin to prepare nanofibrous scaffolds. [17][18][19][20][21][22][23] Up to now, very limited work has been reported on nonmulberry silk fibroin blends due to difficulties associated with its dissolution and processing.…”

Section: Introductionmentioning

confidence: 99%

“…have produced 3D printable bio ink by blending B. mori silk fibroin and gelatin for cartilage tissue engineering 15 . The mechanical properties of B. mori silk fibroin/gelatin scaffolds have been studied by Bagrov et al 16 . A few researchers have electrospun B. mori silk fibroin/gelatin to prepare nanofibrous scaffolds 17–23 …”

Section: Introductionmentioning

confidence: 99%

Deduction of a facile method to construct Antheraea mylitta silk fibroin/gelatin blend films for prospective biomedical applications

Batra

Purwar

2020

Polymer International

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Blend films of two types (I and II) were prepared by mixing Antheraea mylitta silk fibroin (AMF) and gelatin solution in various blend ratios via the solution casting method. Two different crosslinkers, namely glutaraldehyde and genipin, were used during blend preparation. The structural characteristics and thermal properties of the blend films were examined by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Diffrential scanning calorimetery (DSC). The FTIR spectra showed conformational alterations in type I blend films while type II films attained high ⊎-sheet crystallinity. The XRD diffractograms presented a high degree of crystallinity in type II blend films compared to type I, which showed an almost amorphous structure. Further, thermal and biological studies were conducted on type II films. According to the TGA thermograms, the degradation temperature of the crosslinked blend films shifted compared to pure gelatin and pure AMF films. Partial miscibility of the two components was indicated by DSC thermograms of the blends. The high water uptake capacity of type II blend films was found to imitate hydrogel behaviour. The blend films did not show any toxicity in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and supported L929 fibroblast cell spreading and proliferation. The biodegradation of the blend films was significantly faster than the pure silk film.

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Mechanical properties of films and three-dimensional scaffolds made of fibroin and gelatin

Cited by 6 publications

References 42 publications

Effects of Chemical Post-treatments on Structural and Physicochemical Properties of Silk Fibroin Films Obtained From Silk Fibrous Waste

Effects of Chemical Post-treatments on Structural and Physicochemical Properties of Silk Fibroin Films Obtained From Silk Fibrous Waste

Bioresorbable Scaffolds Based on Fibroin for Bone Tissue Regeneration

Deduction of a facile method to construct Antheraea mylitta silk fibroin/gelatin blend films for prospective biomedical applications

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