Mechanical properties of polycaprolactone (PCL) scaffolds for hybrid 3D-bioprinting with alginate-gelatin hydrogel

Koch, Fritz; Thaden, Ole; Conrad, Stefan; Tröndle, Kevin; Finkenzeller, Günter; Zengerle, Roland; Kartmann, Sabrina; Zimmermann, Stefan; Koltay, Peter

doi:10.1016/j.jmbbm.2022.105219

Cited by 30 publications

(16 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Figure 3D, the scaffold surface is divided by microchannels to allow the flow of liquids or gases, and the latter is essential for vascularization. 42 There is diverse literature reporting that the pore sizes that promote osteogenesis range from 100 to 1000 µm. 41,43 Although there is no consensus on the ideal pore size in bone regeneration, some studies reveal that the pore size that favors the proliferation of adhesion and even influences osteogenic differentiation is 100-400 µm 30,44 Lutzweiler et al 45 points out that porous 3D environments provide several signals that can affect cells.…”

Section: Discussionmentioning

confidence: 99%

“…However, it is noteworthy that molecular weight, filament diameter, and extrusion temperature influence the mechanical properties of 3D printed scaffolds, and it has also been reported that increasing porosity leads to a decrease in elastic properties. 42 However, it is essential to recall that the cortical tissue of the skull does not receive significant mechanical forces, as would the cortical bone of the femur or other anatomical sites.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds

Pérez‐Sánchez,

Ortiz de la O,

Álvarez‐Pérez

et al. 2024

MedComm – Biomaterials and Applications

View full text Add to dashboard Cite

The challenge of three‐dimensional (3D) printing by polymeric extrusion in tissue bioengineering is to control with precision the microarchitecture and porous interconnectivity of scaffolds, as well as search for models that allow and facilitate the development of personalized constructs that meet optimal characteristics for the regeneration of significant bone defects. In this study, anatomically accurate scaffolds were designed and printed to a critical size defect from a microtomography image of the rat calvaria. Different software is used to design a scaffold with exact anatomy. With Ultimaker Cura software, distinct printing parameters were standardized, permitting the printing of different types of pores and graded porosity scaffolds, with exact adaptation to the bone defect, utilizing a commercial 3D printer with a fused deposition modeling technique and compensating for the limitations of the method. The scaffolds were characterized by evaluating their mechanical properties and surface characteristics (pore size and porosity), employing scanning electron microscopy images, verifying that the size and shape of the pores were controlled, and evaluating cell viability and cell distribution on the 3D printed scaffold. Therefore, this work proves that by standardizing the printing parameters, it was possible to print a unique customized scaffold, controlling the shape and size of pores.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds

Pérez‐Sánchez,

Ortiz de la O,

Álvarez‐Pérez

et al. 2024

MedComm – Biomaterials and Applications

View full text Add to dashboard Cite

show abstract

“…Processability and good mechanical properties, high crystallinity and low melting point, excellent rheological properties, and viscoelasticity endow it with good melt printing ability ( Cui et al, 2021 ; Hamid et al, 2021 ). Polycaprolactone also can store and restore deformation, It can adapt to the rapid development of 3D printing technology, is suitable for making tissue engineering scaffolds, and becomes a common material for biological 3D printing ( Koch et al, 2022 ; Xu et al, 2023 ). Microstructural scaffolds designed with polycaprolactone as raw materials can provide structural support and transport channels to induce tissue regeneration ( Li et al, 2022b ).…”

Section: Polymer Materials For 3d Bio Printing Of Bone and Cartilagementioning

confidence: 99%

3D printing of bone and cartilage with polymer materials

Fan

Liu²,

Wang³

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Damage and degeneration to bone and articular cartilage are the leading causes of musculoskeletal disability. Commonly used clinical and surgical methods include autologous/allogeneic bone and cartilage transplantation, vascularized bone transplantation, autologous chondrocyte implantation, mosaicplasty, and joint replacement. 3D bio printing technology to construct implants by layer-by-layer printing of biological materials, living cells, and other biologically active substances in vitro, which is expected to replace the repair mentioned above methods. Researchers use cells and biomedical materials as discrete materials. 3D bio printing has largely solved the problem of insufficient organ donors with the ability to prepare different organs and tissue structures. This paper mainly discusses the application of polymer materials, bio printing cell selection, and its application in bone and cartilage repair.

show abstract

“…The use of PCL or PCL blends as feedstock in additive manufacturing technology based on the material extrusion process (MEAM, also referred to as 3D Printing—3DP) is reported mainly for applications related to drug delivery or scaffold manufacturing [ 3 , 4 ]. In the existing studies, 3D prints with properties suitable for the bone tissue engineering field are fabricated from specially prepared materials based on PCL [ 5 , 6 , 7 , 8 , 9 ]. An interesting study using state-of-the-art analysis on PCL mixed with antimicrobial metals for 3D-printed customized wound dressing applications [ 10 ] was also found.…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Polycaprolactone Mechanical Characterization and Suitability Assessment for Producing Wrist–Hand Orthoses

et al. 2023

View full text Add to dashboard Cite

In this research, the mechanical properties of 3D-printed polycaprolactone (PCL), a biocompatible and biodegradable semi-crystalline polyester, available as feedstock for additive manufacturing technology based on the material extrusion process, were determined. The influence of the infill pattern (zig-zag vs. gyroid) and ultraviolet (UV-B) exposure over the specimens’ mechanical performances were also investigated to gather relevant data on the process parameter settings for different applications. Specimens and samples of 3D-printed PCL were analyzed through tensile and flexural tests. The experimental data showed the good repeatability of the manufacturing process, as well as a mechanical behavior independent of the specimens’ infill pattern at full density. No differences between the failure patterns of the tensile specimens were recorded. UV-B exposure proved to have a significant negative impact on the specimens’ tensile strength. The 3D printing of PCL and PCL blends is reported mainly for use in scaffold manufacturing or drug delivery applications. As another novelty, the suitability of commercial PCL filaments for producing patient-customized wrist–hand orthoses was also assessed in this study. Semi-cylindrical PCL samples mimicking the forearm part of a wrist–hand orthosis with hexagonal open pockets were 3D-printed and mechanically tested. The results were discussed in comparison to samples with a similar design, made of polylactic acid. The experiments revealed the need to carefully calibrate the manufacturing parameters to generate defect-free, good quality prints. Once settings were established, promising results were obtained when producing orthoses in a ready-to-use form. On the other hand, the attempts to thermoform flat 3D-printed PCL orthoses proved unsuccessful.

show abstract

Mechanical properties of polycaprolactone (PCL) scaffolds for hybrid 3D-bioprinting with alginate-gelatin hydrogel

Cited by 30 publications

References 32 publications

Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds

Standardization of 3D printing parameters to control the size and shape of pores in Polylactic acid scaffolds

3D printing of bone and cartilage with polymer materials

3D-Printed Polycaprolactone Mechanical Characterization and Suitability Assessment for Producing Wrist–Hand Orthoses

Contact Info

Product

Resources

About