Mechanical properties of porous crosslinked poly(ethyl-acrylate) for tissue engineering

Estellés, Jorge Más; Krakovský, Ivan; Hernandez, Jesus Corral; Piotrowska, A.; Pradas, Manuel Monleón

doi:10.1007/s10853-007-1727-2

Cited by 14 publications

(14 citation statements)

References 20 publications

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“…Las características de la estructura molecular del PEA, movilidad de la cadena lateral, la polaridad y su baja hidrofilicidad, permiten que tenga lugar una interacción directa entre el polímero y las proteínas de la matriz extracelular. Por otra parte, es posible llevar a cabo la preparación de materiales ultraporosos, que permiten la invasión celular y que se utilizan como guías para el crecimiento celular, son los "scaffolds" (21)(22)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36) . Además mediante distintas variantes de la técnica de porógeno-lixiviación se consiguen distintas estructuras porosas (27-29, 31, 33-36) .…”

Section: Estrategias Actuales De Regeneración Neural: Uso De "Scaffolunclassified

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Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Gamboa-MartÍnez

Ribelles

Ferrer

2011

Journal of Bioactive and Compatible Polymers

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A hybrid scaffold was obtained by the deposition of a thin network of submicron fibrin fibrils on the microporous walls of a macroporous poly(L-lactide) (PLLA) three-dimensional structure. The fibrin coating is homogeneous across the entire substrate and allowed the pore structure remain open in the hybrid scaffold. The elastic modulus of the hybrid scaffold (0.65 MPa) was increased up to twofold compared to the pure PLLA scaffold (0.29 MPa). Mouse pre-osteoblastic cells, MC3T3, were seeded on both pure PLLA and hybrid scaffolds, and cultured for 3, 6, and 24 h. The coating enhanced the cell colonization and proliferation and provided a more homogeneous distribution of cells within the scaffolds. In addition, the coating improved the scaffold adhesion properties by supplying new binding sites to the cells that modify the transmembrane receptors involved in initial cell adhesion mechanism. The expression of the β3 integrin was observed in cells cultured on fibrin-coated scaffolds instead of the α5 integrin, which was expressed in the uncoated scaffold. These hybrid PLLA/fibrin scaffolds have cell culture features suitable to promote early tissue regeneration.

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Section: Estrategias Actuales De Regeneración Neural: Uso De "Scaffolunclassified

“…Además, el tamaño de los poros y la geometría, no modifican las propiedades de este tipo de "scaffolds" de PEA. El diámetro de los poros tampoco interfiere en las propiedades mecánicas del material, siempre que no se llegue a colapsar el poro durante la compresión (29) .…”

Section: Estrategias Actuales De Regeneración Neural: Uso De "Scaffolunclassified

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Gamboa-MartÍnez

Ribelles

Ferrer

2011

Journal of Bioactive and Compatible Polymers

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“…Next, samples were incubated with 2% osmium tetraoxide for 2 h at room temperature followed by four rinses with distilled water. Afterwards, samples were dehydrated through a series of graded ethanol (30,50,70,96, and 100) at 4ºC. Samples were finally dried by criticalpoint drying and sputter-coated with gold to be observed by SEM at 15 kV and 15 mm of working distance.…”

Section: Analysis Of the Cell Distribution In The Scaffold-cum-gel Comentioning

confidence: 99%

“…It has shown excellent compatibility with different types of cells in vitro: chondrocytes [18], keratinocytes [19], endothelial cells [20], neural cells [21][22][23][24], osteoblasts [25] or dental pulp stem cells [26], and has been recently proposed as a feeder-free platform for the maintenance and growth of human embryonic stem cells [27]. Due to its easy way of polymerization, PEA can be prepared in the form of scaffolds with different architectures, liable to invasion by cells and intended as guiding support for cell growth [1,19,20,22,23,[28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

Vallés-Lluch¹,

Arnal-Pastor²,

Martínez‐Ramos³

et al. 2013

Acta Biomaterialia

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ElsevierVallés Lluch, A.; Arnal Pastor, MP.; Martínez Ramos, C.; Vilariño Feltrer, G.; Vikingsson, L.; Castells Sala, C.; Semino, CE.... (2013). Combining self-assembling peptide gels with three-dimensional elastomer scaffolds. Acta Biomaterialia. 9 (12) Abstract: Some of the problems raised by the combination of porous scaffolds and self-assembling peptide (SAP) gels as constructs for tissue engineering applications are for the first time addressed. Scaffolds of poly(ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physico-chemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold's pores was assessed, and the kinetics of its release in PBS was followed. Cell seeding and colonization of these constructs was preliminary studied with L929 fibroblasts and checked afterwards with sheep adipose-tissue derived stem cells (ASCs) intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffoldcum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the 3D structure. These constructs could be of use in different advanced tissue engineering applications where, apart from a cell-friendly ECM-like aqueous environment, a larger-scale three-dimensional structure able to keep the cells in a specific place, give mechanical support and/or conduct spatially the tissue growth could be required. COMBINING SELF-ASSEMBLING PEPTIDE GELS WITH 3D ELASTOMER SCAFFOLDSA. AbstractSome of the problems raised by the combination of porous scaffolds and selfassembling peptide (SAP) gels as constructs for tissue engineering applications are for the first time addressed. Scaffolds of poly(ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physicochemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold's pores was assessed, and the kinetics of its release in PBS was followed. Cell intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffold-cum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the 3D structure. These constructs could be of use in different advanced tissue engineering applications where, apart from a cell-friendly ECM-like aqueous environment, a larger-scale three-dimensional structure able to keep the cells in a specific place, give mechanical support and/o...

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“…In comparison with other scaffold morphologies, this cylindrical structure is more stable against the pore collapse phenomenon which takes place with other fabrication procedures [7,142]. Furthermore, it is expected that the mechanical properties of the scaffolds we are dealing with should be larger than those corresponding to other pore geometries [143,144], mainly due to less porosity values (~60% versus ~80% for interconnected spherical pores). The manufacturing method used gives rise to well-ordered porous structures which can be made of several materials, including composites.…”

Section: Nanocomposite Scaffolds With Ordered Cylindrical Orthogonal mentioning

confidence: 97%

Acrylate-silica polymer nanocomposites obtained by sol-gel reactions. Structure, properties and scaffold preparation.

Rodríguez¹,

Carlos²

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Mechanical properties of porous crosslinked poly(ethyl-acrylate) for tissue engineering

Cited by 14 publications

References 20 publications

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Fibrin coating on poly (L-lactide) scaffolds for tissue engineering

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

Acrylate-silica polymer nanocomposites obtained by sol-gel reactions. Structure, properties and scaffold preparation.

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