Mechanical properties of single cells: Measurement methods and applications

Hao, Yansheng; Cheng, Shaokoon; Tanaka, Yo; Hosokawa, Yoichiroh; Yalikun, Yaxiaer; Li, Ming

doi:10.1016/j.biotechadv.2020.107648

Cited by 69 publications

(48 citation statements)

References 215 publications

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“…Measurements of forces have been critical in enabling the growing field of mechanobiology (Table 1). [134][135][136][137][138][139] There are two types of tools for force measurements: one measures the forces actively generated…”

Section: Force Measurementsmentioning

confidence: 99%

“…3D, three dimensional; ECM, extracellular matrix; dECM, decellularized ECM; hESC, human embryonic stem cell; hTSC, human trophoblast stem cell [Colour figure can be viewed at wileyonlinelibrary.com] by cells without applying any external force, and the other measures cellular responses to mechanical forces or mechanical quantities such as cell stiffness. [135][136][137][138][139][140] Over the past 20 years, a wide repertoire of techniques has been developed to measure forces. [135][136][137][138][139][140] These techniques, based mostly on ex vivo and in vitro conditions, have been extensively reviewed in.…”

Section: Force Measurementsmentioning

confidence: 99%

“…[135][136][137][138][139][140] Over the past 20 years, a wide repertoire of techniques has been developed to measure forces. [135][136][137][138][139][140] These techniques, based mostly on ex vivo and in vitro conditions, have been extensively reviewed in. [135][136][137][138][139][140] However, these techniques are still far from becoming routine biological laboratory tools because multidisciplinary expertise is required to conduct and interpret the measurements.…”

Section: Force Measurementsmentioning

confidence: 99%

“…[135][136][137][138][139][140] These techniques, based mostly on ex vivo and in vitro conditions, have been extensively reviewed in. [135][136][137][138][139][140] However, these techniques are still far from becoming routine biological laboratory tools because multidisciplinary expertise is required to conduct and interpret the measurements. [135][136][137][138][139][140] Furthermore, quantitative comparisons of the mechanical properties measured by different methods are limited.…”

Section: Force Measurementsmentioning

confidence: 99%

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Mechanobiology of the female reproductive system

Matsuzaki

2021

Reprod Medicine & Biology

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The field of mechanobiology focuses on how the responses of cells, tissues, and organs to mechanical cues, resulting from both intracellularly generated and externally generated forces, contribute to development, differentiation, physiology and disease via the integration of medicine, biology, engineering, and physics. [1][2][3] How living cells can sense their environment and adequately respond in terms of morphology, migration, proliferation, differentiation, and survival requires understanding at multiple scales, from molecules to single cells, tissues, and organs. [1][2][3] More than a century ago, mechanical forces were proposed to drive embryogenesis and bone structure. [1][2][3] The importance of mechanical forces for biological regulation was recognized in the field of developmental biology at the beginning of the twentieth century. [1][2][3] However, there were no experimental tools available to directly test

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Section: Force Measurementsmentioning

confidence: 99%

Section: Force Measurementsmentioning

confidence: 99%

Section: Force Measurementsmentioning

confidence: 99%

Section: Force Measurementsmentioning

confidence: 99%

See 2 more Smart Citations

Mechanobiology of the female reproductive system

Matsuzaki

2021

Reprod Medicine & Biology

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“…Also, to make these techniques more applicable and practical for biologists, and to promote using cell mechanics as a biomarker, improvements are required for the technique to be automatic, high throughput while being robust, accurate, and sensitive with high time and space resolutions. This might be done by coupling the optical microelastography technique with other methods, such as microfluidics with a high throughput [400,401].…”

Section: Cellular Shear Wave Elastographymentioning

confidence: 99%

Viscoelasticity Imaging of Biological Tissues and Single Cells Using Shear Wave Propagation

Flé

Bhatt

et al. 2021

Front. Phys.

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Changes in biomechanical properties of biological soft tissues are often associated with physiological dysfunctions. Since biological soft tissues are hydrated, viscoelasticity is likely suitable to represent its solid-like behavior using elasticity and fluid-like behavior using viscosity. Shear wave elastography is a non-invasive imaging technology invented for clinical applications that has shown promise to characterize various tissue viscoelasticity. It is based on measuring and analyzing velocities and attenuations of propagated shear waves. In this review, principles and technical developments of shear wave elastography for viscoelasticity characterization from organ to cellular levels are presented, and different imaging modalities used to track shear wave propagation are described. At a macroscopic scale, techniques for inducing shear waves using an external mechanical vibration, an acoustic radiation pressure or a Lorentz force are reviewed along with imaging approaches proposed to track shear wave propagation, namely ultrasound, magnetic resonance, optical, and photoacoustic means. Then, approaches for theoretical modeling and tracking of shear waves are detailed. Following it, some examples of applications to characterize the viscoelasticity of various organs are given. At a microscopic scale, a novel cellular shear wave elastography method using an external vibration and optical microscopy is illustrated. Finally, current limitations and future directions in shear wave elastography are presented.

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Label‐Free Impedance Analysis of Induced Pluripotent Stem Cell‐Derived Spinal Cord Progenitor Cells for Rapid Safety and Efficacy Profiling

He,

Tan,

et al. 2024

Adv Materials Technologies

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Regenerative therapies, including the transplantation of spinal cord progenitor cells (SCPCs) derived from induced pluripotent stem cells (iPSCs), are promising treatment strategies for spinal cord injuries. However, the risk of tumorigenicity from residual iPSCs advocates an unmet need for rapid SCPCs safety profiling. Herein, a rapid (≈3000 cells min‐1) electrical‐based microfluidic biophysical cytometer is reported to detect low‐abundance iPSCs from SCPCs at single‐cell resolution. Based on multifrequency impedance measurements (0.3 to 12 MHz), biophysical features including cell size, deformability, membrane, and nucleus dielectric properties are simultaneously quantified as a cell is hydrodynamically stretched at a cross junction under continuous flow. A supervised uniform manifold approximation and projection (UMAP) model is further developed for impedance‐based quantification of undifferentiated iPSCs with high sensitivity (≈1% spiked iPSCs) and shows good correlations with SCPCs differentiation outcomes using two iPSC lines. Cell membrane opacity (day 1) is also identified as a novel early intrinsic predictive biomarker that exhibits a strong correlation with SCPC differentiation efficiency (day 10). Overall, it is envisioned that this label‐free and optic‐free platform technology can be further developed as a versatile cost‐effective process analytical tool to monitor or assess stem cell quality and safety in regenerative medicine.

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Mechanical properties of single cells: Measurement methods and applications

Cited by 69 publications

References 215 publications

Mechanobiology of the female reproductive system

Mechanobiology of the female reproductive system

Viscoelasticity Imaging of Biological Tissues and Single Cells Using Shear Wave Propagation

Label‐Free Impedance Analysis of Induced Pluripotent Stem Cell‐Derived Spinal Cord Progenitor Cells for Rapid Safety and Efficacy Profiling

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