Mechanical properties of single living cells encapsulated in polyelectrolyte matrixes

Lanero, Tiziana Svaldo; Cavalleri, Ornella; Krol, Silke; Rolandi, R.; Gliozzi, Alessandra

doi:10.1016/j.jbiotec.2006.02.016

Cited by 83 publications

(26 citation statements)

References 38 publications

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“…Loading–unloading curves almost overlaped. This observation is in agreement with previous studies (Charras et al, 2001; Chen et al, 2010; Favre et al, 2011; Svaldo Lanero et al, 2006). …”

Section: Resultssupporting

confidence: 94%

Mechanical properties of human amniotic fluid stem cells using nanoindentation

Aryaei¹,

Jayasuriya²

2013

Journal of Biomechanics

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The aim of this study was to obtain nanomechanical properties of living cells focusing on human amniotic fluid stem (hAFS) cell using nanoindentation techniques. We modified the conventional method of atomic force microscopy (AFM) in aqueous environment for cell imaging and indentation to avoid inherent difficulties. Moreover, we determined the elastic modulus of murine osteoblast (OB6) cells and hAFS cells at the nucleus and cytoskeleton using force–displacement curves and Hertz theory. Since OB6 cell line has been widely used, it was selected to validate and compare the obtained results with the previous research studies. As a result, we were able to capture high resolution images through utilization of the tapping mode without adding protein or using flxation methods. The maximum depth of indentation was kept below 15% of the cell thickness to minimize the effect of substrate hardness. Nanostructural details on the surface of cells were visualized by AFM and fluorescence microscopy. The cytoskeletal fibers presented remarkable increase in elastic modulus as compared with the nucleus. Furthermore, our results showed that the elastic modulus of hAFS cell edge (31.6 kPa) was lower than that of OB6 cell edge (42.2 kPa). In addition, the elastic modulus of nucleus was 13.9 kPa for hAFS cell and 26.9 kPa for OB6 cells. Differences in cell elastic modulus possibly resulted from the type and number of actin cytoskeleton organization in these two cell types.

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“…Loading–unloading curves almost overlaped. This observation is in agreement with previous studies (Charras et al, 2001; Chen et al, 2010; Favre et al, 2011; Svaldo Lanero et al, 2006). …”

Section: Resultssupporting

confidence: 94%

Mechanical properties of human amniotic fluid stem cells using nanoindentation

Aryaei¹,

Jayasuriya²

2013

Journal of Biomechanics

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“…From these force curves we obtain the elastic deformation as a function of the loading force applied by the tip. Then, the Young's modulus (E) was calculated for each force from the approaching part of the force curves only, as the force curves recorded for growth cones exhibited a hysteresis that in general evidences the viscoelastic behavior of the sample [45]. For each force curve the contact point between the tip and the growth cone was determined using a home made implemented Igor code added to the Asylum Research software.…”

Section: Methodsmentioning

confidence: 99%

Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury

Martin

Benzina²,

Szabo³

et al. 2013

PLoS ONE

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A prior peripheral nerve injury in vivo, promotes a rapid elongated mode of sensory neurons neurite regrowth in vitro. This in vitro model of conditioned axotomy allows analysis of the cellular and molecular mechanisms leading to an improved neurite re-growth. Our differential interference contrast microscopy and immunocytochemistry results show that conditioned axotomy, induced by sciatic nerve injury, did not increase somatic size of adult lumbar sensory neurons from mice dorsal root ganglia sensory neurons but promoted the appearance of larger neurites and growth cones. Using atomic force microscopy on live neurons, we investigated whether membrane mechanical properties of growth cones of axotomized neurons were modified following sciatic nerve injury. Our data revealed that neurons having a regenerative growth were characterized by softer growth cones, compared to control neurons. The increase of the growth cone membrane elasticity suggests a modification in the ratio and the inner framework of the main structural proteins.

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“…These assumptions can be made under our experimental conditions, and thus the Hertz–Sneddon model is appropriate to use in our case. Further details on the theoretical basis for this model can be found in several references 66,70,79…”

Section: Introductionmentioning

confidence: 99%

Effects of Morphology vs. Cell–Cell Interactions on Endothelial Cell Stiffness

Stroka

Aranda‐Espinoza

2010

Cel. Mol. Bioeng.

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Biological processes such as atherogenesis, wound healing, cancer cell metastasis, and immune cell transmigration rely on a delicate balance between Cell-Cell and cell-substrate adhesion. Cell mechanics have been shown to depend on substrate factors such as stiffness and ligand presentation, while the effects of Cell-Cell interactions on the mechanical properties of cells has received little attention. Here, we use atomic force microscopy to measure the Young's modulus of live human umbilical vein endothelial cells (HUVECs). In varying the degree of Cell-Cell contact in HUVECs (single cells, groups, and monolayers), we observe that increased cell stiffness correlates with an increase in cell area. Further, we observe that HUVECs stiffen as they spread onto a glass substrate. When we weaken Cell-Cell junctions (i.e., through a low dose of cytochalasin B or treatment with a VE-cadherin antibody), we observe that cell-substrate adhesion increases, as measured by focal adhesion size and density, and the stiffness of cells within the monolayer approaches that of single cells. Our results suggest that while morphology can roughly be used to predict cell stiffness, Cell-Cell interactions may play a significant role in determining the mechanical properties of individual cells in tissues by careful maintenance of cell tension homeostasis.

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Mechanical properties of single living cells encapsulated in polyelectrolyte matrixes

Cited by 83 publications

References 38 publications

Mechanical properties of human amniotic fluid stem cells using nanoindentation

Mechanical properties of human amniotic fluid stem cells using nanoindentation

Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury

Effects of Morphology vs. Cell–Cell Interactions on Endothelial Cell Stiffness

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