Mechanical property analysis of stored red blood cell using optical tweezers

Li, Yanjie; Wen, Cheng P.; Xie, Huimin; Ye, Anpei; Yin, Yajun

doi:10.1016/j.colsurfb.2008.11.012

Cited by 45 publications

(34 citation statements)

References 11 publications

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“…Laser Tweezer Measurement of Cell Membrane Tension-We measured the tether force with optical tweezers as described previously (40). The key components consist of a 2000 milliwatt Nd:YAG laser source (1064 nm) connected to a 100ϫ oilimmersion inverted microscope (Zeiss), highly sensitive cold CCD camera (Cascade), and a three-dimensional piezoelectric ceramic stage.…”

Section: Methodsmentioning

confidence: 99%

“…Our collective observations thus imply an increase in membrane movement in MLCK-deficient SMCs. As membrane tension is an important factor for the modulation of membrane dynamics (18,46), we then directly measured the tether force of SMC membrane with laser tweezers (40). This method did not work accurately for bleb-forming cells; therefore, we only chose the cells without obvious bleb formation for tether force measurement.…”

Section: Characterization Of Mlck-deficientmentioning

confidence: 99%

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Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Chen

Tao

Wen

et al. 2014

Journal of Biological Chemistry

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Background: MLCK in cell migration remains controversial. Results: MLCK deletion causes enhanced cell protrusion along with a reduction of membrane tension and is rescued by kinase-dead MLCK or five-DFRXXL motif. Conclusion: MLCK regulates cell migration not by myosin regulatory light chain phosphorylation but possibly through a membrane tension-based mechanism. Significance: Our results shed light on a novel regulatory mechanism of protrusion during cell migration.

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Section: Methodsmentioning

confidence: 99%

Section: Characterization Of Mlck-deficientmentioning

confidence: 99%

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Chen

Tao

Wen

et al. 2014

Journal of Biological Chemistry

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“…Since Sylvie Hénon et al measured the shear modulus of single RBC membrane successfully with double optical tweezers [9], plenty of researchers have shifted their sights to study deformability of RBCs with optical tweezers. The recent main research contents include the following several aspects: (1) The influences of sickle cell disease, malaria, diabetes and other blood diseases on RBCs deformation and drug treatment of pathological RBCs [10-13]; (2) The transformation of RBCs deformation under different physiological environments [14,15]; (3) The analysis of RBCs deformation by combining optical tweezers with the finite element simulation [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

“…It's noticeable that the experimental efficiency replies on the sample pretreatment process and the operation complexity of optical tweezers. Through comparing these measurement procedures, we have recognized that double-beads adhesion strategy is superior to other methods due to higher precision and relative controllable adhesion area between the bead and the cell [9,11,15]. In previous reports, some researchers use the modified micro beads as handles to attach the RBCs that cost too much time [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…Through comparing these measurement procedures, we have recognized that double-beads adhesion strategy is superior to other methods due to higher precision and relative controllable adhesion area between the bead and the cell [9,11,15]. In previous reports, some researchers use the modified micro beads as handles to attach the RBCs that cost too much time [14,15]. Certainly the effects of functional modification reagents on the experiment are unknown as well as.…”

Section: Introductionmentioning

confidence: 99%

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Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers

Song¹,

Liu²,

Zhang³

et al. 2016

Biomed. Opt. Express

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Abstract:The elasticity of red cell membrane is a critical physiological index for the activity of RBC. Study of the inherent mechanism for RBCs membrane elasticity transformation is attention-getting all along. This paper proposes an optimized measurement method of erythrocytes membrane shear modulus incorporating acousto-optic deflector (AOD) scanning optical tweezers system. By use of this method, both membrane shear moduli and sizes of RBCs with different in vitro times were determined. The experimental results reveal that the RBCs membrane elasticity and size decline with in vitro time extension. In addition, semi quantitative measurements of S-nitrosothiol content in blood using fluorescent spectrometry during in vitro storage show that RBCs membrane elasticity change is positively associated with the S-nitrosylation level of blood. The analysis considered that the diminished activity of the nitric oxide synthase makes the S-nitrosylation of in vitro blood weaker gradually. The main reason for worse elasticity of the in vitro RBCs is that S-nitrosylation effect of spectrin fades. These results will provide a guideline for further study of in vitro cells activity and other clinical applications. References and links1. N. Hamasaki and M. Yamamoto, "Red blood cell function and blood storage," Vox Sang. 79(4), 191-197 (2000). 2. M. Whirter, J. Liam, H. Noguchi, and G. Gompper, "Ordering and arrangement of deformed red blood cells in flow through microcapillaries," New J. Phys. 14(8), 6709-6717 (2012). 3. M. Musielak, "Red blood cell-deformability measurement: review of techniques," Clin. Hemorheol. Microcirc.42(1), 47-64 (2009). 4. S. Svetina, G. Kokot, T. S. Kebe, B. Žekš, and R. E. Waugh, "A novel strain energy relationship for red blood cell membrane skeleton based on spectrin stiffness and its application to micropipette deformation," Biomech. Model. Mechanobiol. 15(3), 745-758 (2016). 5. G. J. Streekstra, J. G. G. Dobbe, and A. G. Hoekstra, "Quantification of the fraction poorly deformable red blood cells using ektacytometry," Opt. Express 18(13), 14173-14182 (2010). 6. L. Dintenfass, "Internal viscosity of the red cell and a blood viscosity equation," Nature 219(5157), 956-958 (1968). 649-652 (1986). 37. Z. Peng, X. Li, I. V. Pivkin, M. Dao, G. E. Karniadakis, and S. Suresh, "Lipid bilayer and cytoskeletal interactions in a red blood cell," Proc. Natl. Acad. Sci. U.S.A. 110(33), 13356-13361 (2013). 38. M. Grau, P. Friederichs, S. Krehan, C. Koliamitra, F. Suhr, and W. Bloch, "Decrease in red blood cell deformability is associated with a reduction in RBC-NOS activation during storage," Clin. Hemorheol. Microcirc. 60(2), 215-229 (2015).

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Laser interference‐based technique for dynamic measurement of single cell deformation manipulated by optical tweezers

et al. 2018

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A laser interference‐based method was proposed to measure the deformation response of cell manipulated by optical tweezers. This method was implemented experimentally by integrating a laser illuminating system and optical tweezers with an inverted microscope. Interference fringes generated by the transmitted and reflected lights were recorded by a complementary metal oxide semiconductor camera. From the acquired images, cell height was calculated and cell morphology was constructed. To further validate this method, the morphological analyses of HeLa cells were performed in static state and during detachment process. Subsequently, the dynamic deformation responses of red blood cells were measured during manipulation with optical tweezers. Collectively, this laser interference‐based method precludes the requirement of complex optical alignment, allows easy integration with optical tweezers, and enables dynamic measurement of cell deformation response by using a conventional inverted microscope.

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Mechanical property analysis of stored red blood cell using optical tweezers

Cited by 45 publications

References 11 publications

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Myosin Light Chain Kinase (MLCK) Regulates Cell Migration in a Myosin Regulatory Light Chain Phosphorylation-independent Mechanism

Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers

Laser interference‐based technique for dynamic measurement of single cell deformation manipulated by optical tweezers

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