Mechanical response of single myoblasts to various stretching patterns visualized by scanning probe microscopy

Mitsui, Waka; Tamura, Kazushi; Mizutani, Takeomi; Haga, Hisashi; Kawabata, Kazushige

doi:10.1679/aohc.72.227

Cited by 2 publications

(2 citation statements)

References 16 publications

(14 reference statements)

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“…In addition, integrin b1D was also involved in stretch-induced E app elevation in C2C12 cells, since integrin b1D knockdown prohibited the increased E app [66]. Another intriguing study by Mitsui et al [72] displayed a time lapse sequence of stiffness distributions of living myoblasts by scanning probe microscopy when cells were stretched intermittently. They proposed that myoblasts have tensional memory effect to maintain cytoskeletal tensional homeostasis, based on the observation that cell stiffness was increased instantly after stretching but gradually decrease and return to its original level.…”

Section: Genes Regulating Mechanical Properties Of Myoblastsmentioning

confidence: 98%

“…They proposed that myoblasts have tensional memory effect to maintain cytoskeletal tensional homeostasis, based on the observation that cell stiffness was increased instantly after stretching but gradually decrease and return to its original level. In addition, this memory effect, which largely depends on the duration, rather than the magnitude of stretching, might be related with phosphorylation level of myosin II regulatory light chain [72].…”

Section: Genes Regulating Mechanical Properties Of Myoblastsmentioning

confidence: 99%

See 1 more Smart Citation

Multiple Effects of Mechanical Stretch on Myogenic Progenitor Cells

Wang

Song

Liu

et al. 2020

Stem Cells and Development

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Mechanically stretched skeletal muscle undergoes dramatic shifts in structure, mass, and function. In vitro tensile strain models have demonstrated that myogenic progenitor cells, including satellite cells and myoblasts, are highly mechanosensitive cells, and respond to mechanical strain in a wide variety of aspects. However, the experimental results from different researchers and laboratories are not always in support of each other. Moreover, some specific molecules or signaling pathways were reported to play distinct roles in stretched myogenic cells, according to the statements of different studies. The purpose of this review is to integrate the researches conducting in vitro culture of satellite cells or myoblasts and exploring their mechanoresponses using in vitro stretching apparatus. These responses will be categorized into several groups, such as activation, proliferation, myogenic differentiation, cellular damage or apoptosis, properties of plasma membrane, transdifferentiation, reorientation, etc. In addition, detailed experimental designs like culturing conditions and straining regimens will be displayed and compared, to interpret some contradictory statements in different studies. Furthermore, the currently known interconnections among some mechanosensitive pathways will be pictured to give a better understanding about the complex regulations of myogenic cell responses to mechanical stretch. Hopefully, by summarizing the published studies about mechanoresponses of myogenic progenitor cells, future directions, and perspectives would be made clearer to researchers in this field.

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Section: Genes Regulating Mechanical Properties Of Myoblastsmentioning

confidence: 98%

Section: Genes Regulating Mechanical Properties Of Myoblastsmentioning

confidence: 99%