Mechanical shock during egg de-adhesion and post-ovulatory ageing contribute to spontaneous autopolyploidy in white sturgeon culture (Acipenser transmontanus)

Eenennaam, Joel P. Van; Fiske, Aviva J.; Leal, Michaiah J.; Cooley-Rieders, Chessie; Todgham, Anne E.; Conte, Fred S.; Schreier, Andrea

doi:10.1016/j.aquaculture.2019.734530

Cited by 17 publications

(23 citation statements)

References 52 publications

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“…Havelka et al [ 20 ] described the spontaneous duplication of the maternal chromosome set in Siberian sturgeon of different ploidy caused by either premeiotic endomitosis or the retention of the second polar body. Many other studies [ 51 , 52 , 53 ] have stated the retention that the second polar body can lead to polyploidization. The different ratios of triploid/pentaploid individuals among the parent combinations in our study also supported this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny

Káldy

Mozsár

Fazekas

et al. 2020

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Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (Polyodon spathula, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from three sturgeon and sperm from four paddlefish individuals. Survival in all hybrid family groups ranged from 62% to 74% 30 days after hatching. This was the first successful hybridization between these two species and between members of the family Acipenseridae and Polyodontidae. Flow cytometry and chromosome analysis revealed two ploidy levels in hybrids. The chromosome numbers of the hybrids ranged between 156–184 and 300–310, in “functional” triploids and “functional” pentaploids, respectively. The hybrid origin and the ploidy levels were also confirmed by microsatellite analyses. In hybrids, the size and the number of dorsal and ventral scutes correlated with the ploidy levels as well as with the calculated ratio of the maternal and paternal chromosome sets. An extra haploid cell lineage was found in three hybrid individuals irrespective of the ploidy level, suggesting polyspermy. Although the growth performance showed high variance in hybrids (mean: 1.2 kg, SD: 0.55), many individuals reached a size of approximately 1 kg by the age of one year under intensive rearing conditions.

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Section: Discussionmentioning

confidence: 99%

Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny

Káldy

Mozsár

Fazekas

et al. 2020

Genes

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“…Ploidy variation in fish has been observed to occur spontaneously (Gold & Avise, 1976; Machado et al, 2012; Utsunomia et al, 2014). Some artificial spawning and rearing practices may increase the rate of spontaneous ploidy variation (Aegerter & Jalabert, 2004; Cherfas, Gomelsky, Ben-Dom, & Hulata, 1995; Delomas & Dabrowski, 2016; Flajšhans, Kvasnicka, & Ráb, 1993; Glover et al, 2015; Thorgaard et al, 1982; Van Eenennaam et al, 2020) which could lead to more ploidy variation in systems stocked with hatchery-origin fish. Alterations in ploidy can also be induced in fish and shellfish to yield individuals with advantageous qualities for cultivation, such as sterility (Benfey, 1999; Nell, 2002), or stocking in water bodies where reproduction of stocked fish is undesirable (Cassinelli, Meyer, Koenig, Vu, & Campbell, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Our motivation stems from the case of white sturgeon ( Acipenser transmontanus ). White sturgeon are ancestral octoploids (8n) (Drauch Schreier, Gille, Mahardja, & May, 2011), but spontaneous autopolyploidy has been observed in hatchery settings, producing dodecaploids (12n) (Van Eenennaam et al, 2020). Crossing individuals with these two ploidy levels then yields decaploids (10n).…”

Section: Introductionmentioning

confidence: 99%

Inference of ploidy by leveraging read depth from amplicon sequencing

Delomas

Willis

Schreier

et al. 2020

Preprint

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Variation in ploidy occurs naturally in select plant and animal species. Ploidy variation can also occur spontaneously or be induced during artificial propagation of fish and shellfish. Studying species and systems that have variable ploidy requires techniques to infer ploidy of individuals. Massively parallel sequencing of biallelic SNPs has been used to infer ploidy, but existing techniques have several drawbacks. These include being limited to only comparing a fixed number of ploidies (diploidy, triploidy, and tetraploidy) and requiring that heterozygous genotypes in an individual be identified prior to ploidy inference. We describe a method of inferring ploidy from sequencing of biallelic SNPs based on beta-binomial mixture models. This method is generalized to apply to any ploidy and does not require prior identification of heterozygous genotypes. We demonstrate efficacy of this method for comparing ancestral octoploidy, decaploidy, and dodecaploidy (tetraploidy, pentaploidy, and hexaploidy for the sequenced SNPs) in white sturgeon and diploidy and triploidy in Chinook salmon with amplicon sequencing (GT-seq) data. Results indicated that ploidy could be reliably estimated for individuals based on distinct distribution of log-likelihood ratios (LLR) for known ploidy samples of both species that were tested. Confidence in ploidy estimates increased with sequencing depth. We encourage users to explore the sequencing depths and LLR critical values that provide reliable estimates of ploidy for a given organism and set of SNPs. We expect that the R package provided will empower studies of genetic variation and inheritance in organisms that vary in ploidy naturally or as a result of artificial propagation practices.

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“…D. Schreier, May, & Gille, 2013). And though of unknown fertility, backcrossed offspring (10N, decaploid) are often viable (J. P. Van Eenennaam et al, 2019), creating a wide range of ploidies within a single species.…”

Section: Introductionmentioning

confidence: 99%

Single nucleotide polymorphism genotypes and ploidy estimates for ploidy variable species generated with massively parallel amplicon sequencing

Willis¹,

Delomas²,

Parker³

et al. 2020

Preprint

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Polyploidization has played a critical role in the evolution of several major organism groups, including vertebrates, but much of our knowledge of the evolution of polyploids comes from allopolyploid and often rediploidized lineages, which partly reflects the difficulty of obtaining genotype data from polysomic genomes. We combined several contemporary methods to develop markers for single nucleotide polymorphisms compatible with simultaneous ploidy estimation and high throughput genotyping, and analyzed these data with recent software developments that accept polysomic data. We demonstrate the utility of this combination to develop genetic resources for polysomic species by applying it to the ploidy-variable and polysomic white sturgeon (Acipenser transmontanus), an imperiled species under conservation management in the Pacific Northwest. We introduce a primer and probe set for 325 SNP markers for use with the 'Genotyping-by-thousands' (GT-seq) method, and provide updated scripts that incorporate a function to estimate ploidy from each individual using read count data. We examine the reliability of tetrasomic inheritance in a large sample of paleo-octoploid individuals and the expected Mendelian inheritance patterns in known cross families. We then demonstrate our ability to use these data to infer parentage, relatedness, and other population genetic parameters. Our combined process thus improves the accessibility of genetic information to facilitate future investigations of white sturgeon and is expected to be widely applicable to other polyploid species.

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Mechanical shock during egg de-adhesion and post-ovulatory ageing contribute to spontaneous autopolyploidy in white sturgeon culture (Acipenser transmontanus)

Cited by 17 publications

References 52 publications

Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny

Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny

Inference of ploidy by leveraging read depth from amplicon sequencing

Single nucleotide polymorphism genotypes and ploidy estimates for ploidy variable species generated with massively parallel amplicon sequencing

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