“…Beneficial outcomes were shown for thermal pretreatment (Camacho et al, 2005;Phothilangka et al, 2008;Tunçal, 2011), addition of enzymes (Barjenbruch and Kopplow, 2003;Roman et al, 2006), ozonation (Song et al, 2003;Carballa et al, 2007), chemical solubilization by acidification (Chen et al, 2007), alkaline hydrolysis (Grübel et al, 2013;Mukherjee and Levine, 1992;Vlyssides and Karlis, 2004), alkaline addition and sonication (Jean et al, 2000;Zhang et al, 2007) as well as mechanical and ultrasonic sludge disintegration (Coelho et al, 2014;Grübel and Machnicka, 2009;Kampas et al, 2007;Kumar et al, 2000;Wang et al, 2006).…”