We present spatially resolved radio-frequency spectroscopy of a trapped Fermi gas with resonant interactions and observe a spectral gap at low temperatures. The spatial distribution of the spectral response of the trapped gas is obtained using in situ phase-contrast imaging and 3D image reconstruction. At the lowest temperature, the homogeneous rf spectrum shows an asymmetric excitation line shape with a peak at 0.48(4)εF with respect to the free atomic line, where εF is the local Fermi energy. Radio-frequency (rf) spectroscopy measures an excitation spectrum by inducing transitions to different hyperfine spin states. This method has been employed in strongly interacting Fermi gases, leading to the observation of unitarity limited interactions [7,8], molecule formation on the BEC side of the Feshbach resonance [9] as well as pairing in the crossover regime [10,11]. Rf spectroscopy provides valuable information on the nature of the pairs. Since an rf photon can dissociate bound molecules or fermion pairs into the free atom continuum, the binding energy of the pairs or the excitation gap is determined. Furthermore the excitation line shape is related to the wave function of the pairs, e.g. larger pairs have narrower lines. However, currently all experimental measurements on the excitation spectrum in strongly interacting Fermi gases [10,12] have been performed with samples confined in a harmonic trapping potential so that the spectral line shape is broadened due to the inhomogeneous density distribution of the trapped samples, preventing a more stringent comparison with theoretical predictions [13,14,15,16].In this Letter, we demonstrate spatially resolved rf spectroscopy of a trapped, population-balanced Fermi gas at unitarity at very low temperature. The spatial distribution of the rf-induced excited region in the trapped gas was recorded with in situ phase-contrast imaging [17] and the local rf spectra were compiled after 3D image reconstruction. In contrast to the inhomogeneous rf spectrum, the homogeneous local rf spectrum shows a clear spectral gap with an asymmetric line shape. We observe that the spectral peak shifts by 0.48(4)ε F to higher energy and that the spectral gap is 0.30(8)ε F with respect to the free atomic reference line, where ε F is the local Fermi energy. This new spectroscopic method overcomes the line broadening problem for inhomogeneous samples and provides homogeneous rf spectra of a resonantly interacting Fermi gas revealing the microscopic physics of fermion pairs.We prepared a degenerate Fermi gas of spin-polarized 6 Li atoms in an optical trap, using laser cooling and sympathetic cooling with 23 Na atoms, as described in Ref. [18]. An equal mixture of the two lowest hyperfine states |1 and |2 (corresponding to the |F = 1/2, m F = 1/2 and |F = 1/2, m F = −1/2 states at low magnetic field) was created at a magnetic field B = 885 G. A broad Feshbach resonance located at B 0 = 834 G strongly enhanced the interactions between the two states. The final evaporative cooling by lowering the t...