Mechanical vibrations increase the proliferation of articular chondrocytes in high-density culture

Kaupp, Jake; Waldman, Stephen D.

doi:10.1243/09544119jeim376

Cited by 32 publications

(35 citation statements)

References 53 publications

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“…It is known that mechanical vibrations increase the proliferation of articular chondrocytes in high-density cultures of isolated bovine articular chondrocytes [6]. In addition, vibration promoted differentiation of rat pheochromocytoma (PC12) cells, which supported the hypothesis that effects of the physical environment can influence cellular differentiation [28].…”

Section: Discussionmentioning

confidence: 55%

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Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Lützenberg

Solano

Buken

et al. 2018

Cell Physiol Biochem

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Background/Aims: Spaceflight negatively influences the function of cartilage tissue in vivo. In vitro human chondrocytes exhibit an altered gene expression of inflammation markers after a two-hour exposure to vibration. Little is known about the impact of long-term vibration on chondrocytes. Methods: Human cartilage cells were exposed for up to 24 h (VIB) on a specialised vibration platform (Vibraplex) simulating the vibration profile which occurs during parabolic flights and compared to static control conditions (CON). Afterwards, they were investigated by phase-contrast microscopy, rhodamine phalloidin staining, microarray analysis, qPCR and western blot analysis. Results: Morphological investigations revealed no changes between CON and VIB chondrocytes. F-Actin staining showed no alterations of the cytoskeleton in VIB compared with CON cells. DAPI and TUNEL staining did not identify apoptotic cells. ICAM-1 was elevated and vimentin, beta-tubulin and osteopontin proteins were significantly reduced in VIB compared to CON cells. qPCR of cytoskeletal genes, ITGB1, SOX3, SOX5, SOX9 did not reveal differential regulations. Microarray analysis detected 13 differentially expressed genes, mostly indicating unspecific stimulations. Pathway analyses demonstrated interactions of PSMD4 and CNOT7 with ICAM. Conclusions: Long-term vibration did not damage human chondrocytes in vitro. The reduction of osteopontin protein and the down-regulation of PSMD4 and TBX15 gene expression suggest that in vitro long-term vibration might even positively influence cultured chondrocytes.

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Section: Discussionmentioning

confidence: 55%

“…It has been demonstrated that mechanical vibrations can elevate the proliferation of chondrocytes in monolayer cultures [6]. These vibration effects seem to be limited to the early stages where ECM (collagen and proteoglycans) accumulation is at a minimum [6].…”

Section: Introductionmentioning

confidence: 99%

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Lützenberg

Solano

Buken

et al. 2018

Cell Physiol Biochem

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“…Recently, Kaupp and Waldman [22] reported that mechanical vibrations increase the proliferation of bovine articular chondrocytes. In addition, Puig et al [23] indicated that the release of interleukin-8 in human bronchial epithelial cells is enhanced by subjecting the cells to mechanical vibrations during culture.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Cytoplasmic Maturation of In Vitro-Matured Pig Oocytes by Mechanical Vibration

Mizobe

Yoshida

Miyoshi

2010

Journal of Reproduction and Development

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Abstract. The effects of mechanical vibration during in vitro maturation and/or in vitro culture after artificial activation of pig oocytes on maturation and development were examined. In addition, the optimal conditions were applied to in vitro production of blastocysts derived from miniature pig somatic cell nuclear transfer (SCNT) embryos. Mechanical vibration during in vitro maturation did not affect the rates (60.5 ± 1.9-69.5 ± 2.2%) of oocytes reaching the metaphase-II stage. However, the blastocyst formation rates after activation of oocytes matured with mechanical vibration for 5 sec at intervals of 30-60 min or for 10 sec at intervals of 60 min were significantly (P<0.05) higher than those of oocytes matured without mechanical vibration (25.7 ± 2.0-28.1 ± 2.7% vs. 12.3 ± 1.4% and 25.8 ± 1.8% vs. 15.7 ± 1.9%, respectively). In contrast, mechanical vibration during in vitro culture after activation did not affect the blastocyst formation (11.6 ± 5.2-16.5 ± 3.0%) of oocytes. Mechanical vibration for 5 sec at intervals of 60 min during in vitro maturation of oocytes did not affect fusion (66.8 ± 3.5-72.1 ± 3.1%) with miniature pig somatic cells after enucleation. However, the blastocyst formation rate of SCNT embryos was improved (P<0.05) by mechanically vibrating recipient oocytes for 5 sec at intervals of 60 min during in vitro maturation, regardless of the presence or absence of the same treatment during in vitro culture (17.6 ± 2.5% vs. 9.4 ± 0.9% and 13.0 ± 0.3% vs. 7.4 ± 0.9%, respectively). The results indicated that mechanical vibration enhances the cytoplasmic maturation of in vitro-matured pig oocytes, resulting in improvement of their parthenogenetic development. In addition, it was shown that in vitro maturation of oocytes with mechanical vibration can be applied to efficient production of blastocysts derived from miniature pig SCNT embryos. he low cloning efficiency associated with the development of somatic cell nuclear transfer (SCNT) embryos to offspring remains the major obstacle to use of this technology in various fields of animal science and biomedical applications. Establishment of systems to support efficient production of blastocysts from SCNT embryos in vitro is quite important for basic research to clarify the mechanism controlling the development of SCNT embryos, which will bring about improvement of cloning efficiency. In cattle and pigs, in vitro oocyte maturation systems produce an abundant and stable supply of recipient oocytes for SCNT because immature oocytes can be obtained from slaughtered animals. In vitro-matured oocytes have been commonly used for production of cloned calves [1-3] and pigs [4][5][6][7][8]. The oocyte maturation process is a crucial step for the generation of oocytes capable of being fertilized and undergoing normal embryonic development into blastocysts after in vitro fertilization [9]. Therefore, efficient production of blastocysts from SCNT embryos requires optimization of both recipient oocyte maturation systems and SCNT embryo culture systems.Oocyte ...

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“…Other studies have illustrated that mechanical stimuli applied for short durations increase chondrocyte metabolism and that the cells become less sensitive if the stimulus is applied for longer periods of time (habituation response). 26,[53][54][55] It is generally recognized that mechanosensitive cells of the mesenchymal lineage rapidly desensitize to applied mechanical stimuli which has long been established in the case of bone cells. 43,44,47 Long-term application of intermittent static BTS led to measurable changes in the physical and biochemical properties of the developed tissues which were dependent on the frequency of strain application.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Intermittent Static Biaxial Tensile Strains on Tissue Engineered Cartilage

Fan

Waldman

2010

Ann Biomed Eng

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Mechanical stimulation of engineered cartilage constructs is a commonly applied method used to accelerate tissue formation and improve the mechanical properties of the developed tissue. While the effects of compression and shear have been widely studied, the effect of tension has received relatively little attention. As articular cartilage in vivo is subjected to a degree of static tension (pre-tension) even in the absence of externally applied loads, the purpose of this study was to investigate the effect of intermittent static biaxial tensile strains (BTS) on chondrocyte metabolism and resultant tissue formation. Using a custom-design loading fixture to apply BTS, the optimal conditions for stimulating extracellular matrix synthesis were under average magnitudes of 3.8% radial and 2.1% circumferential tensile strains for 30 min. Tissue constructs subjected to tensile strain stimulation 3 times/week for a period of 4 weeks displayed increased thickness (35 +/- 18%) and proteoglycan content (22 +/- 7%) without an associated change in mechanical properties. In contrast, constructs stimulated daily over the same time period exhibited negligible effects in terms of ECM accumulation suggesting that the frequency of stimulation needs to be precisely controlled. The results of this study demonstrate that while tension can be used as potential biomechanical stimulus to improve tissue formation, further optimization of this process needs to be conducted to improve ECM accumulation and tissue mechanical properties after long-term exposure to tensile stimuli.

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Mechanical vibrations increase the proliferation of articular chondrocytes in high-density culture

Cited by 32 publications

References 53 publications

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

Enhancement of Cytoplasmic Maturation of In Vitro-Matured Pig Oocytes by Mechanical Vibration

The Effect of Intermittent Static Biaxial Tensile Strains on Tissue Engineered Cartilage

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