Mechanically-enhanced polysaccharide-based scaffolds for tissue engineering of soft tissues

Souza, Renata Francielle Bombaldi de; Souza, Fernanda Carla Bombaldi de; Rodrigues, Cristiano; Drouin, Bernard; Popat, Ketul C.; Mantovani, Diego; Moraes, Ângela Maria

doi:10.1016/j.msec.2018.09.045

Cited by 47 publications

(15 citation statements)

References 44 publications

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“…[ 4 ] In these circumstances, the soft tissue becomes relaxed and loses its supporting function. [ 5 ] Traditional surgical treatment is invasive, and has been eliminated due to various complications and susceptibility to relapse caused by forced pulling and suture fixing; [ 6 ] allotransplantation was then considered, however, the goal of soft tissue functional recovery was still unmet because it merely fills the defect. [ 7 ] Therefore, the minimally invasive approach for loose soft tissue (LST) regeneration involving structural integrity and functional reconstruction has always been a challenge in clinic.…”

Section: Introductionmentioning

confidence: 99%

Heat‐Shrinkable Electrospun Fibrous Tape for Restoring Structure and Function of Loose Soft Tissue

Saiding

Jin

Qin

et al. 2020

Adv Funct Materials

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Non‐invasive recovery of loose soft tissue caused by trauma, organ expansion, or compression has not been solved properly. Herein, a new approach of heat‐shrinkable electrospun fibrous tape (HS‐EFT) is constructed via electrospinning in which the contraction of the poly(lactic‐co‐glycolic acid) HS‐EFT causes the relaxed fascia to pull back at body temperature while the mechanical force of retraction is transformed into an external signal that regulates the behavior of fibroblasts. As it contracts more than 40% within five days, the fast proliferation of fibroblasts on the contracted fibrous membrane is observed followed by the formation of more stress fibers, consequently promoting the nuclear transfer of mechanical stimulation‐sensitive co‐transcriptional molecule, yes‐associated protein (YAP), and eventually making new extracellular matrix (ECM) deposition, as well as tissue strengthen. Moreover, in the process of fascia defect repairing in rabbits, the loose tissue is gathered by fibrous tape which ends in a reduced wound area as well as tissue repairing. Therefore, the HS‐EFT provides great inspiration for the reconstruction of relaxed soft tissue both structurally and functionally in a non‐invasive way.

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Section: Introductionmentioning

confidence: 99%

Heat‐Shrinkable Electrospun Fibrous Tape for Restoring Structure and Function of Loose Soft Tissue

Saiding

Jin

Qin

et al. 2020

Adv Funct Materials

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“…The use of two of more biopolymers, associated to form inter penetrating networks, complexes, multilayers or fibers is a promising alternative as it does not imply the use of potentially toxic chemicals (Luan et al, 2017;Rahmani Del Bakhshayesh et al, 2018;Bombaldi de Souzaa et al, 2019). Among these possibilities the formation of polyelectrolyte complexes (PEC), involving a negatively charged polymer and a positively charged polymer is one of the most attractive strategies in terms of gain in mechanical properties without loss of biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Alginate-chitosan PEC scaffolds: A useful tool for soft tissues cell therapy

Bushkalova

Farno

Tenailleau

et al. 2019

International Journal of Pharmaceutics

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In this study we evaluate macroporous scaffolds made of alginate-chitosan polyelectrolyte complexes (PEC) as tools to optimire the results of soft tissues oell therapy. Cell therapy using mesenchymal stem cells (MSC) bas become attractive for tissue repair and regeneration in a number of acute and chronic injuries. Unfortunately their low retention and/or survival after injection limit their beneficial effects. A biomaterial-assisted im plantation, providi ng oells a thr�mensional (3D) microenvironment is a promising strategy. To this purpose, we designed a family of PEC scaffolds, and studied if they could meet the requirement of such application. Xray tomography showed that ail PEC scaffolds present an interconnected macroporosity, and both rheology and tensile measurements reveal optimized mechanical properties (higher storage moduli and Young moduli) compared to alginate referenoe scaffolds. In vitro assays demonstrated their ability to allow MSC retention (higher than 90%) , lo ng-term viability and RiF2 secretion. Then, we used a skeletal muscle implantation mode! to assess the biological response to scaffolds graft, and showed that they support in vivo vascular formation within the implant-derived tissue. The combination of alginate/chitosan PEC scaffolds architecture and angio genic potential make them appear as interesti ng tools to optimize MSC therapy results in soft tissues.

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“…Its polar groups and physicochemical properties provide a favorable non-protein environment for cell adhesion and proliferation [83]. It easily forms a blend with other polymers through electrostatic interactions and confers antimicrobial properties to the final composition [1,21,84].…”

Section: Scaffolds Developed From Polysaccharidesmentioning

confidence: 99%

“…Soft tissues are complex fiber-reinforced structures, generally distinguishable from hard tissues by their high water content [1]. They are continuously invaded by trauma, invasive surgery, and aging.…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide Based Scaffolds for Soft Tissue Engineering Applications

2018

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Soft tissue reconstructs require materials that form three-dimensional (3-D) structures supportive to cell proliferation and regenerative processes. Polysaccharides, due to their hydrophilicity, biocompatibility, biodegradability, abundance, and presence of derivatizable functional groups, are distinctive scaffold materials. Superior mechanical properties, physiological signaling, and tunable tissue response have been achieved through chemical modification of polysaccharides. Moreover, an appropriate formulation strategy enables spatial placement of the scaffold to a targeted site. With the advent of newer technologies, these preparations can be tailor-made for responding to alterations in temperature, pH, or other physiological stimuli. In this review, we discuss the developmental and biological aspects of scaffolds prepared from four polysaccharides, viz. alginic acid (ALG), chitosan (CHI), hyaluronic acid (HA), and dextran (DEX). Clinical studies on these scaffolds are also discussed.

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Mechanically-enhanced polysaccharide-based scaffolds for tissue engineering of soft tissues

Cited by 47 publications

References 44 publications

Heat‐Shrinkable Electrospun Fibrous Tape for Restoring Structure and Function of Loose Soft Tissue

Heat‐Shrinkable Electrospun Fibrous Tape for Restoring Structure and Function of Loose Soft Tissue

Alginate-chitosan PEC scaffolds: A useful tool for soft tissues cell therapy

Polysaccharide Based Scaffolds for Soft Tissue Engineering Applications

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