Mechanically Strong Globular‐Protein‐Based Fibers Obtained Using a Microfluidic Spinning Technique

He, Haonan; Yang, Chenjing; Wang, Fan; Zheng, Wei; Shen, Jianlei; Chen, Dong; Fan, Chunhai; Zhang, Hongjie; Li, Kai

doi:10.1002/anie.201915262

Cited by 68 publications

(56 citation statements)

References 45 publications

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“…This behavior is in agreement with the synchrotron radiation small‐angle X‐ray scattering (SR‐SAXS) analysis (Figure E), confirming the ordered alignment of the proteins in the fibers. All these results suggest that long‐range ordered internal structures can be realized by post‐stretching treatment (Figure F) …”

Section: Figurementioning

confidence: 68%

Bioinspired and Mechanically Strong Fibers Based on Engineered Non‐Spider Chimeric Proteins

Sun

et al. 2020

Angew Chem Int Ed

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Silk‐protein‐based fibers have attracted considerable interest due to their low weight and extraordinary mechanical properties. Most studies on fibrous proteins focus on the recombinant spidroins, but these fibers exhibit moderate mechanical performance. Thus, the development of alternative structural proteins for the construction of robust fibers is an attractive goal. Herein, we report a class of biological fibers produced using a designed chimeric protein, which consists of the sequences of a cationic elastin‐like polypeptide and a squid ring teeth protein. Remarkably, the chimeric protein fibers exhibit a breaking strength up to about 630 MPa and a corresponding toughness as high as about 130 MJ m−3, making them superior to many recombinant spider silks and even comparable to some native counterparts. Therefore, this strategy is a novel concept for exploring bioinspired ultrastrong protein materials through the development of new types of structural chimeric proteins.

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Section: Figurementioning

confidence: 68%

Bioinspired and Mechanically Strong Fibers Based on Engineered Non‐Spider Chimeric Proteins

Sun

et al. 2020

Angew Chem Int Ed

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“…The fabrication of photo‐switchable bioengineered protein‐surfactant fibers consists of two crucial components: a cationic supercharged polypeptide ( SUP ) forming the structural basis of the fiber material and an anionic sulfonated surfactant containing an azobenzene moiety ( Azo ) (Figure 1 A). The SUPs are derived from natural elastin and were expressed recombinantly in E. coli [40–44] . The high net charge of SUP s is encoded in the pentapeptide repeat unit (VPGKG) n in which the fourth position is consisting of a lysine residue (K).…”

Section: Resultsmentioning

confidence: 99%

“…TheSUPs are derived from natural elastin and were expressed recombinantly in E. coli. [40][41][42][43][44] Theh igh net charge of SUPsi se ncoded in the pentapeptide repeat unit (VPGKG) n in which the fourth position is consisting of al ysine residue (K). Regarding the SUP used for fiber fabrication, we chose K108cys that contains two cysteines at the N-and C-terminus,r espectively.T he digit of K108cys denotes the number of positive charges along the polypeptide backbone ( Figure S1-S3, Table S1).…”

Section: Resultsmentioning

confidence: 99%

Reversibly Photo‐Modulating Mechanical Stiffness and Toughness of Bioengineered Protein Fibers

Sun

Maity

et al. 2020

Angewandte Chemie

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Light‐responsive materials have been extensively studied due to the attractive possibility of manipulating their properties with high spatiotemporal control in a non‐invasive fashion. This stimulated the development of a series of photo‐deformable smart devices. However, it remained a challenge to reversibly modulate the stiffness and toughness of bulk materials. Here, we present bioengineered protein fibers and their optomechanical manipulation by employing electrostatic interactions between supercharged polypeptides (SUPs) and an azobenzene (Azo)‐based surfactant. Photo‐isomerization of the Azo moiety from the E‐ to Z‐form reversibly triggered the modulation of tensile strength, stiffness, and toughness of the bulk protein fiber. Specifically, the photo‐induced rearrangement into the Z‐form of Azo possibly strengthened cation–π interactions within the fiber material, resulting in an around twofold increase in the fiber's mechanical performance. The outstanding mechanical and responsive properties open a path towards the development of SUP‐Azo fibers as smart stimuli‐responsive mechano‐biomaterials.

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“…Hydrogels with an open network structure can absorb a large amount of water, making them more suitable for promoting mixing and reaction [ 37 , 38 , 39 ]. Recently, a hydrogel prepared from bovine serum albumin (BSA) has attracted widespread attention because of its low cost, facile preparation, and excellent accessibility of the ligand binding [ 40 , 41 , 42 ]. It was found that BSA hydrogel microspheres prepared through the spray-drying of glutaraldehyde (GA) cross-linked BSA showed strong autofluorescence [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Production of Autofluorescent BSA Hydrogel Microspheres and Their Sequential Trapping for Fluorescence-Based On-Chip Permanganate Sensing

Liu

Xiang

et al. 2020

Sensors

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Microfabrication technologies have extensively advanced over the past decades, realizing a variety of well-designed compact devices for material synthesis, separation, analysis, monitoring, sensing, and so on. The performance of such devices has been undoubtedly improved, while it is still challenging to build up a platform by rationally combining multiple processes toward practical demands which become more diverse and complicated. Here, we present a simple and effective microfluidic system to produce and immobilize a well-defined functional material for on-chip permanganate (MnO4−) sensing. A droplet-based microfluidic approach that can continuously produce monodispersed droplets in a water-in-oil system is employed to prepare highly uniform microspheres (average size: 102 μm, coefficient of variation: 3.7%) composed of bovine serum albumin (BSA) hydrogel with autofluorescence properties in the presence of glutaraldehyde (GA). Each BSA hydrogel microsphere is subsequently immobilized in a microchannel with a hydrodynamic trapping structure to serve as an independent fluorescence unit. Various anions such as Cl−, NO3−, PO43−, Br−, BrO3−, ClO4−, SCN−, HCO3−, and MnO4− are individually flowed into the microchannel, resulting in significant fluorescence quenching only in the case of MnO4−. Linear correlation is confirmed at an MnO4− concentration from 20 to 80 μM, and a limit of detection is estimated to be 1.7 μM. Furthermore, we demonstrate the simultaneous immobilization of two kinds of different microspheres in parallel microchannels, pure BSA hydrogel microspheres and BSA hydrogel microspheres containing rhodamine B molecules, making it possible to acquire two fluorescence signals (green and yellow). The present microfluidics-based combined approach will be useful to record a fingerprint of complicated samples for sensing/identification purposes by flexibly designing the size and composition of the BSA hydrogel microspheres, immobilizing them in a desired manner and obtaining a specific pattern.

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Mechanically Strong Globular‐Protein‐Based Fibers Obtained Using a Microfluidic Spinning Technique

Cited by 68 publications

References 45 publications

Bioinspired and Mechanically Strong Fibers Based on Engineered Non‐Spider Chimeric Proteins

Bioinspired and Mechanically Strong Fibers Based on Engineered Non‐Spider Chimeric Proteins

Reversibly Photo‐Modulating Mechanical Stiffness and Toughness of Bioengineered Protein Fibers

Microfluidic Production of Autofluorescent BSA Hydrogel Microspheres and Their Sequential Trapping for Fluorescence-Based On-Chip Permanganate Sensing

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