Mechanics and surface ultrastructure changes of poly(3-hydroxybutyrate) films during enzymatic degradation in pancreatic lipase solution

Жуйков, В. А.; Бонарцев, А. П.; Bagrov, D. V.; Yakovlev, Sergey; Мышкина, В. Л.; Махина, Т. К.; Bessonov, I. V.; Kopitsyna, M. N.; Morozov, A. V.; Rusakov, A. A.; Усеинов, А. С.; Шайтан, К. В.; Бонарцева, Г. А.

doi:10.1080/15421406.2017.1302580

Cited by 21 publications

(18 citation statements)

References 21 publications

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“…It is well known − that hydrolytic degradation begins in the amorphous phase. Molecules of water and lipase in PBS interact with ester bonds in the amorphous regions, thereby reducing the polymer’s molecular mass. − The cleaved shorter chains possess greater chain mobility, which facilitates the crystallization process in the amorphous phase (Figure i).…”

Section: Resultsmentioning

confidence: 99%

“…It is well known 48 that when amorphous chains in hydrolyzable polymers are broken into shorter ones under biodegradation conditions, oligomeric products are generated that can diffuse out of the polymer bulk and dissolve in the medium (Figure 12iv). Thus, a release of the amorphous phase takes place leaving the polymer more crystalline.…”

Section: Characterization Of the Electrospun Composite Phb/fe 3 O 4 S...mentioning

confidence: 99%

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Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance

Mukhortova

Chernozem

et al. 2022

ACS Appl. Bio Mater.

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Magnetically responsive composite polymer scaffolds have good potential for a variety of biomedical applications. In this work, electrospun composite scaffolds made of polyhydroxybutyrate (PHB) and magnetite (Fe 3 O 4 ) particles (MPs) were studied before and after degradation in either PBS or a lipase solution. MPs of different sizes with high saturation magnetization were synthesized by the coprecipitation method followed by coating with citric acid (CA). Nanosized MPs were prone to magnetite−maghemite phase transformation during scaffold fabrication, as revealed by Raman spectroscopy; however, for CA-functionalized nanoparticles, the main phase was found to be magnetite, with some traces of maghemite. Submicron MPs were resistant to the magnetite−maghemite phase transformation. MPs did not significantly affect the morphology and diameter of PHB fibers. The scaffolds containing CA-coated MPs lost 0.3 or 0.2% of mass in the lipase solution and PBS, respectively, whereas scaffolds doped with unmodified MPs showed no mass changes after 1 month of incubation in either medium. In all electrospun scaffolds, no alterations of the fiber morphology were observed. Possible mechanisms of the crystalline-lamellar-structure changes in hybrid PHB/Fe 3 O 4 scaffolds during hydrolytic and enzymatic degradation are proposed. It was revealed that particle size and particle surface functionalization affect the mechanical properties of the hybrid scaffolds. The addition of unmodified MPs increased scaffolds' ultimate strength but reduced elongation at break after the biodegradation, whereas simultaneous increases in both parameters were observed for composite scaffolds doped with CA-coated MPs. The highest saturation magnetization�higher than that published in the literature�was registered for composite PHB scaffolds doped with submicron MPs. All PHB scaffolds proved to be biocompatible, and the ones doped with nanosized MPs yielded faster proliferation of rat mesenchymal stem cells. In addition, all electrospun scaffolds were able to support angiogenesis in vivo at 30 days after implantation in Wistar rats.

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Section: Resultsmentioning

confidence: 99%

Section: Characterization Of the Electrospun Composite Phb/fe 3 O 4 S...mentioning

confidence: 99%

Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance

Mukhortova

Chernozem

et al. 2022

ACS Appl. Bio Mater.

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“…All the samples were examined under neutral and enzymatic degradations in phosphate buffered saline (PBS, pH 7.2) at 37 °C. Lipase is widely used in transesterification and hydrolysis of ester, which has been researched in polymer degradation such as poly(ε-caprolactone) and poly(3-hydroxybutyrate). − We used the lipase from porcine pancreas to study the enzymatic degradation in 37 °C which is a suitable temperature for maintaining enzymatic activity. The remaining weight and changes in MWs of PIATs were monitored during this course, and their values are presented in Figure a–d.…”

Section: Resultsmentioning

confidence: 99%

Biodegradable Copolyesters with Unexpected Highly Blocky Microstructures and Enhanced Thermal Properties

Zhang

Chen

et al. 2022

ACS Sustainable Chem. Eng.

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To synthesize novel biodegradable polyesters with high heat resistance and good crystallization capability, we designed a series of aliphatic-aromatic copolyesters, i.e., poly-(isoidide-2,5-dimethylene adipate-co-terephthalate)s (PIATs) based on isoidide-2,5-dimethanol (IIDML), a rigid carbohydratebased building block. The M n values and intrinsic viscosities of PIAT copolyesters containing up to 40 mol % aromatic moieties are in the range of 6 000−19 000 g•mol −1 and 0.5−0.87 dL•g −1 , respectively. Interestingly, the products were obtained as blocky copolymers after the one-pot melt polycondensation process because of the thermodynamic equilibrium of IIDML moieties driven by the insufficient thermal stability of the IIDML moieties. In addition, all the copolyesters are semicrystalline with crystallinity from 28% to 55% and tunable T m values ranging from 88 °C to a remarkable 189 °C. They exhibit better (bio)degradability than the same type of polyesters. This work shows that this green monomer plays a key role in balancing thermal properties (especially T g ) and (bio)degradability, and these polyesters potentially broaden the application toward, for example, fibers.

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“…The degree of crystallinity is known to have a tremendous impact on the degradation rates and mechanical properties of PHB. − Therefore, it is crucial to understand the effect of the fiber diameter and of the addition of the filler on the degree of crystallinity of PHB scaffolds. In this regard, the DSC analysis of pure and composite scaffolds with various fiber diameters was conducted next (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…The crystallinity of polymers is reported to influence their biodegradation rate and mechanical properties. , Generally, in semicrystalline polymers, a higher degree of crystallinity yields a lower content of free volume and therefore an increase in stiffness . PHB is a semicrystalline thermoplastic polymer, which, in contrast to amorphous polymers, can crystallize from a melt or solution in the form of spherulites, and the crystallization is time-dependent .…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Study on the Reinforcement of Electrospun PHB Scaffolds with Composite Magnetic Fe₃O₄–rGO Fillers: Structure, Physico-Mechanical Properties, and Piezoelectric Response

et al. 2022

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This is a comprehensive study on the reinforcement of electrospun poly(3-hydroxybutyrate) (PHB) scaffolds with a composite filler of magnetite–reduced graphene oxide (Fe3O4–rGO). The composite filler promoted the increase of average fiber diameters and decrease of the degree of crystallinity of hybrid scaffolds. The decrease in the fiber diameter enhanced the ductility and mechanical strength of scaffolds. The surface electric potential of PHB/Fe3O4–rGO composite scaffolds significantly increased with increasing fiber diameter owing to a greater number of polar functional groups. The changes in the microfiber diameter did not have any influence on effective piezoresponses of composite scaffolds. The Fe3O4–rGO filler imparted high saturation magnetization (6.67 ± 0.17 emu/g) to the scaffolds. Thus, magnetic PHB/Fe3O4–rGO composite scaffolds both preserve magnetic properties and provide a piezoresponse, whereas varying the fiber diameter offers control over ductility and surface electric potential.

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Mechanics and surface ultrastructure changes of poly(3-hydroxybutyrate) films during enzymatic degradation in pancreatic lipase solution

Cited by 21 publications

References 21 publications

Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance

Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance

Biodegradable Copolyesters with Unexpected Highly Blocky Microstructures and Enhanced Thermal Properties

Comprehensive Study on the Reinforcement of Electrospun PHB Scaffolds with Composite Magnetic Fe₃O₄–rGO Fillers: Structure, Physico-Mechanical Properties, and Piezoelectric Response

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Mechanics and surface ultrastructure changes of poly(3-hydroxybutyrate) films during enzymatic degradation in pancreatic lipase solution

Cited by 21 publications

References 21 publications

Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance

Electrospun Magnetic Composite Poly-3-hydroxybutyrate/Magnetite Scaffolds for Biomedical Applications: Composition, Structure, Magnetic Properties, and Biological Performance

Biodegradable Copolyesters with Unexpected Highly Blocky Microstructures and Enhanced Thermal Properties

Comprehensive Study on the Reinforcement of Electrospun PHB Scaffolds with Composite Magnetic Fe3O4–rGO Fillers: Structure, Physico-Mechanical Properties, and Piezoelectric Response

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Product

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Comprehensive Study on the Reinforcement of Electrospun PHB Scaffolds with Composite Magnetic Fe₃O₄–rGO Fillers: Structure, Physico-Mechanical Properties, and Piezoelectric Response