Mechanics of Leukocyte Deformation and Adhesion to Endothelium in Shear Flow

Dong, Chuan; Cao, Jianliang; Struble, Erika J.; Lipowsky, Herbert H.

doi:10.1114/1.143

Cited by 148 publications

(145 citation statements)

References 28 publications

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“…According to Xue et al 4 , the results of several studies show that inorganic nanoparticles can be deposited on the contact surface and improve the tribological properties of the base oil. It was also noted by Xue et al 4 and Dong et al 5 that nanoparticles exhibit good characteristics of adhesion and reduction of wear, even at concentrations below 2 wt.%.…”

Section: Introductionmentioning

confidence: 92%

Study of oxide nanoparticles as additives for vegetable lubricants

et al. 2014

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Currently, vegetable oils have been studied as biolubricants in order to reach new environmental standards. Besides being non-renewable, mineral oils from petroleum bring consequences to the environment due to its low biodegradability. Thus, the aim of this work is to develop a biolubricant and to add oxide nanoparticles (ZnO and CuO) in order to improve abrasion resistance and friction. This product must be biodegradable and has better performance under boundary lubrication. The methodology consisted of the synthesis of biolubricants using vegetable oils (soybean and sunflower) by epoxidation reaction. The tribological performance was evaluated by HFRR (High Frequency Reciprocating Rig). The developed biolubricants showed good tribological properties besides being more adapted to the environment. Also, it was possible to verify that biolubricants without additives are slightly more tribologically effective than lubricants with additives.

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Section: Introductionmentioning

confidence: 92%

Study of oxide nanoparticles as additives for vegetable lubricants

et al. 2014

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“…Shear stress dictates the forces applied to cells individually (i.e., increase the extent of cell deformation) or on the intermolecular bonds between cells. A previous study (9) has shown that drag force that disrupts individual adhesive bonds between cells reduces when vessel size increases under a constant shear stress. Therefore, when melanoma cells travel with the bloodstream through capillaries, the shear stress would have different effects on the adhesion interaction of melanoma cell with EC.…”

Section: Discussionmentioning

confidence: 99%

Integrin VLA-4 enhances sialyl-Lewis^x/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions

Liang¹,

Dong²

2008

American Journal of Physiology-Cell Physiology

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Liang S, Dong C. Integrin VLA-4 enhances sialyl-Lewis x/a -negative melanoma adhesion to and extravasation through the endothelium under low flow conditions. Am J Physiol Cell Physiol 295: C701-C707, 2008. First published July 16, 2008 doi:10.1152/ajpcell.00245.2008.-During their passage through the circulatory system, tumor cells undergo extensive interactions with various host cells including endothelial cells. The capacity of tumor cells to form metastasis is related to their ability to interact with and extravasate through endothelial cell layers, which involves multiple adhesive interactions between tumor cells and endothelium (EC). Thus it is essential to identify the adhesive receptors on the endothelial and melanoma surface that mediate those specific adhesive interactions. P-selectin and E-selectin have been reported as adhesion molecules that mediate the cell-cell interaction of endothelial cells and melanoma cells. However, not all melanoma cells express ligands for selectins. In this study, we elucidated the molecular constituents involved in the endothelial adhesion and extravasation of sialyl-Lewis x/a -negative melanoma cell lines under flow in the presence and absence of polymorphonuclear neutrophils (PMNs). Results show the interactions of ␣4␤1 (VLA-4) on sialylLewis x/a -negative melanoma cells and vascular adhesion molecule (VCAM-1) on inflamed EC supported melanoma adhesion to and subsequent extravasation through the EC in low shear flow. These findings provide clear evidence for a direct role of the VLA-4/ VCAM-1 pathway in melanoma cell adhesion to and extravasation through the vascular endothelium in a shear flow. PMNs facilitated melanoma cell extravasation under both low and high shear conditions via the involvement of distinct molecular mechanisms. In the low shear regime, ␤2-integrins were sufficient to enhance melanoma cell extravasation, whereas in the high shear regime, selectin ligands and ␤ 2-integrins on PMNs were necessary for facilitating the melanoma extravasation process. tumor cell extravasation; vascular adhesion molecule-1; leukocytes; inflammation MALIGNANT MELANOMA has a high propensity for metastatic spread making it the most deadly form of skin cancer. An important step in the metastatic process is the arrest of melanoma cells in the venous or capillary bed of the target organ and subsequent extravasation (3, 5). Therefore, it is important to identify and characterize adhesive receptors on the endothelial and melanoma surfaces that mediate their specific adhesive interactions. P-selectin and E-selectin have been reported as adhesion molecules that mediate the cell-cell interaction of endothelial cells (EC) and melanoma cells (35,36). Members of the selectin family are structurally related, containing a lectin-binding domain that recognizes specific carbohydrates (30). However, not all melanoma cells express ligands for selectins (29, 37), which raised the question as to whether selectin-mediated binding of melanoma cell to the EC is necessary for initiating the adhesi...

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“…50 Other computational models have demonstrated that there are numerous determinants of cell rolling velocities, in addition to a receptor's affinity for its ligand. 49 Previous work has predicted the effects of leukocyte deformability on adhesion kinetics, 18,39 as well as the kinetics of integrin and L-selectin mediated neutrophil aggregation under flow. 107 All of these computational models are highly detailed and mechanistically informative, but they neglect the overall spatial and temporal dynamics within entire tissues.…”

Section: Discussionmentioning

confidence: 99%

Multi-cell Agent-based Simulation of the Microvasculature to Study the Dynamics of Circulating Inflammatory Cell Trafficking

2007

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Abstract-Leukocyte trafficking through the microcirculation and into tissues is central in angiogenesis, inflammation, and the immune response. Although the literature is rich with mechanistic detail describing molecular mediators of these processes, integration of signaling events and cell behaviors within a unified spatial and temporal framework at the multicell tissue-level is needed to achieve a fuller understanding. We have developed a novel computational framework that combines agent-based modeling (ABM) with a network flow analysis to study monocyte homing. A microvascular network architecture derived from mouse muscle was incorporated into the ABM. Each individual cell was represented by an individual agent in the simulation. The network flow model calculates hemodynamic parameters (blood flow rates, fluid shear stress, and hydrostatic pressures) throughout the simulated microvascular network. These are incorporated into the ABM to affect monocyte transit through the network and chemokine/cytokine concentrations. In turn, simulated monocytes respond to their local mechanical and biochemical environments and make behavioral decisions based on a rule set derived from independent literature. Simulated cell behaviors give rise to emergent leukocyte rolling, adhesion, and extravasation. Molecular knockout simulations were performed to validate the model, and predictions of monocyte adhesion, rolling, and extravasation show good agreement with the independently published corresponding mouse studies.

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Mechanics of Leukocyte Deformation and Adhesion to Endothelium in Shear Flow

Cited by 148 publications

References 28 publications

Study of oxide nanoparticles as additives for vegetable lubricants

Study of oxide nanoparticles as additives for vegetable lubricants

Integrin VLA-4 enhances sialyl-Lewis^x/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions

Multi-cell Agent-based Simulation of the Microvasculature to Study the Dynamics of Circulating Inflammatory Cell Trafficking

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Mechanics of Leukocyte Deformation and Adhesion to Endothelium in Shear Flow

Cited by 148 publications

References 28 publications

Study of oxide nanoparticles as additives for vegetable lubricants

Study of oxide nanoparticles as additives for vegetable lubricants

Integrin VLA-4 enhances sialyl-Lewisx/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions

Multi-cell Agent-based Simulation of the Microvasculature to Study the Dynamics of Circulating Inflammatory Cell Trafficking

Contact Info

Product

Resources

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Integrin VLA-4 enhances sialyl-Lewis^x/a-negative melanoma adhesion to and extravasation through the endothelium under low flow conditions